Iminodiacetic Acid
Mostrando 1-12 de 16 artigos, teses e dissertações.
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1. EPR study of gamma induced radicals in amino and iminodiacetic acid derivatives
In this study, electron paramagnetic resonance spectroscopy was used to investigate free radicals formed in gamma irradiated L-glutamine hydrochloride, iminodiacetic acid hydrochloride and N-(2-hyroxyethyl) iminodiacetic acid powders. The free radicals produced in L-glutamine hydrochloride powders were attributed to the CH2CHCOOH radical; and those in iminod
Brazilian Journal of Physics. Publicado em: 2009-09
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2. Selective separation of indium by iminodiacetic acid chelating resin
Indium can be recovered by treating residues, flue dusts, slags, and metallic intermediates in zinc smelting. This paper investigates the adsorption characteristics of indium and iron on an iminodiacetic acid chelating resin, Amberlite®IRC748 (Rohm and Haas Co.-USA). High concentrations of iron are always present in the aqueous feed solution of indium recov
Brazilian Journal of Chemical Engineering. Publicado em: 2007-06
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3. Purification of the recombinant rabies virus G glycoprotein (GPV) produced by Drosophila melanogaster S2 cells using immobilized metal ion affinity chromatography / Purificação da glicoproteina G (GPV) recombinante do virus da raiva produzida por celulas de Drosophila melanogaster S2 atraves de cromatografia de afinidade por ions metalicos imobilizados
Rabies or hydrophobia is a viral infection that affects the central nervous system, occuring in animals and humans. The main proteins found in rabies virus that activates the immunological system are the N nucleoprotein (NPV) and the G glycoprotein, a transmembrane protein that forms the spikes of the virus and induces virus-neutralizing antibodies that prot
Publicado em: 2007
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4. Purificação da glicoproteina G (GPV) recombinante do virus da raiva produzida por celulas de Drosophila melanogaster S2 atraves de cromatografia de afinidade por ions metalicos imobilizados / Purification of the recombinant rabies virus G glycoprotein (GPV) produced by Drosophila melanogaster S2 cells using immobilized metal ion affinity chromatography
Rabies or hydrophobia is a viral infection that affects the central nervous system, occuring in animals and humans. The main proteins found in rabies virus that activates the immunological system are the N nucleoprotein (NPV) and the G glycoprotein, a transmembrane protein that forms the spikes of the virus and induces virus-neutralizing antibodies that prot
Publicado em: 2007
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5. Purification on monoclonal antibodies using IMAC in PEVA hollow fiber membranes: comparison of chelating agents IDA, CM-Asp and TREN / Purificação de anticorpos monoclonais utilizando IMAC em membranas de fibra oca de PEVA : c : omparação dos agentes quelantes IDA, CM-Asp e TREN
Anticorpos monoclonais são imunoglobulinas secretadas por clones de linfócitos B, normais, tumorais ou obtidos pela tecnologia de hibridomas. Os anticorpos monoclonais têm sido utilizados nas áreas analítica e terapêutica, o que implica na necessidade de sua obtenção com pureza superior a 95%. Muitos estudos têm sido realizados visando à purificaç
Publicado em: 2006
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6. IgG purification from human plasma by membrane affinity chromatography with immobilized Cu(II) and Ni(II) : effect of quelators IDA, TREN and CM-Asp / Purificação de IgG a partir do plasma humano por cromatografia em membranas con ions Cu(II) e Ni(II) imobilizados : efeito dos agentes quelantes IDA, TREN e CM-Asp
The use of immunoglobulin G (lgG) in therapeutic applications has growing vastly and it is used in the treatment of a growing number of indications. Several chromatographic techniques have been investigated for IgG purification. In this work, we investigated the effect of the chelators iminodiacetic acid (IDA), carboxymethylaspartate acid (CM-Asp) and Tris-2
Publicado em: 2006
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7. Affinity chromatography with pseudobiospecific ligands on high-performance supports for purification of proteins of biotechnological interest
High-performance affinity matrices were obtained by attaching pseudobiospecific ligands to hollow-fibre membranes. The neutral protease contained in FlavourzymeTM was purified to homogeneity with Yellow 4R-HE affinity hollow-fibre membranes. Immobilisation of Red HE-3B allowed purification of a milk-clotting enzyme obtained by solid-state culture of Mucor ba
Brazilian Journal of Chemical Engineering. Publicado em: 2003-03
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8. Purificação de anticorpos monoclonais anti-TNP do isotipo IgG1 utilizando cromatografia em membranas de afinidade com ions metalicos imobilizados
Monoc1onal antibodies are immunoglobulins produced by a hybrid cell ca11ed hybridoma. These cells result from the fusion of lymphocytes with malignant myeloma cells. Hybridomas cells express both the lymphocyte s property of specific-antibody production and the immortal character of the myeloma cells. Monoc1onal antibodies have been used in analy1ica1 and th
Publicado em: 2002
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9. Surface Plasmon Resonance Imaging-Based Protein Array Chip System for Monitoring a Hexahistidine-Tagged Protein during Expression and Purification
A surface plasmon resonance imaging-based Ni2+-iminodiacetic acid-coated gold chip system was developed to enable specific detection of a hexahistidine-tagged recombinant protein in crude extracts or in column chromatography fractions. This system is especially advantageous for high-throughput analysis of multiple proteins.
American Society for Microbiology.
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10. Effect of Ethylenediaminetetraacetic Acid and Related Chelating Agents on Whole Cells of Gram-Negative Bacteria
The effects of ethylenediaminetetraacetic acid and of four other chelating agents on seven strains of gram-negative bacteria are described. Changes in viability, cell lysis, and release of intracellular materials from suspensions in various buffers, pH 7.8 or 9.2, were observed. Cyclohexane-1,2-diaminotetraacetic acid was the most toxic compound, whereas imi
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11. Selective enrichment of specific DNA, cDNA and RNA sequences using biotinylated probes, avidin and copper-chelate agarose.
We have developed a general procedure for the rapid and efficient enrichment of specific DNA, RNA or cDNA sequences. Biotinylated DNA or RNA is used as a hybridization probe in solution, avidin is then added to label both the probe and hybrid molecules, and the hybridization mixture chromatographed over cupric-iminodiacetic acid agarose beads. Avidin-probe a
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12. Overproduction of Thermus sp. Strain T2 β-Galactosidase in Escherichia coli and Preparation by Using Tailor-Made Metal Chelate Supports
A novel thermostable chimeric β-galactosidase was constructed by fusing a poly-His tag to the N-terminal region of the β-galactosidase from Thermus sp. strain T2 to facilitate its overexpression in Escherichia coli and its purification by immobilized metal-ion affinity chromatography (IMAC). The poly-His tag fusion did not affect the activation, kinetic pa
American Society for Microbiology.