Immobilized Cell Bioreactor
Mostrando 1-12 de 13 artigos, teses e dissertações.
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1. Production of Poly-γ-Glutamate (PGA) Biopolymer by Batch and Semicontinuous Cultures of Immobilized Bacilluslicheniformis strain-R
Production of Polyglutamate (PGA) biopolymer by immobilized Bacilluslicheniformis strain-R was intensively investigated. Preliminary experiments were carried out to address the most suitable immobilization methodology. Entrapment of Bacillus cells in alginate-agar led optimal PGA production (36.75 g/l), with 1.32- and 2.18-fold increase in comparison with al
Brazilian Journal of Microbiology. Publicado em: 2009-12
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2. Produção de estreptavidina recombinante pela levedura Pichia pastoris / Production of recombinant streptavidin by Pichia pastoris yeast
Streptavidin has been exploited as affinity tag to isolate protein in biotinilated columns. Recombinant Pichia pastoris KM71/Stp strains containing the streptavidin core gene were cultured in fed-batch generating 150 g L-1 biomass. This biomass was achieved with a simpler and shorter process that has never been reported. The yeast was pre-cultured into 50 mL
Publicado em: 2006
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3. Estudo da imobilização de células de Saccharomyces cerevisiae em gel de alginato de cálcio no processo de fermentação alcoólica
This work aims to offer a contribution for the development of an economic technology for ethanol production by the biotechnological approach. In this direction, it investigates the conditions for Saccharomyces cerevisiae cells immobilization in calcium alginate and addresses the operational stability aspects of a stirred batch bioreactor in order to deal wit
Publicado em: 2005
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4. Desenvolvimento de modelos estruturados alternativos para o processo de produção de etanol
Compared with growing studies of microorganism populations, few improvements on the development of structured models for product formation have appeared. The objective of the presenting work is the development of altemative dynamic structured models adapted from structured growth models for the ethanol production by fermentation accomplished on a high produc
Publicado em: 2001
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5. IMMOBILIZED Saccharomyces cerevisiae AND Leuconostoc oenus FOR ALCOHOLIC AND MALOLACTIC FERMENTATION IN CONTINUOUS WINE MAKING
The use of sugarcane stalks to immobilize microorganisms by adsorption in continuous fermentation was investigated. The canes were peeled and cut horizontally and transversally into pieces 2 × 1 × 1 cm3. Scanning electron microscopy (SEM) was employed to verify the external and internal structure of the sugarcane stalk support prior to cell immobilization.
Publicado em: 1999
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6. Estudo da transferencia de massa num reator trifasico de leito fluidizado circulante na oxidação do etanol e acido acetico com celulas imobilizadas de Acetobacter S.P
The objective of this work is the study of the kinetic and oxygen transfer in the oxidation of ethanol to acetic acid by immobilized cells, in a circulating three phase fluidized bed bioreactor. In this context experiences with Acetobacter cells, free and immobilized by entrapment in sodium aginate were done. In the first phase of the study, a strain at Acet
Publicado em: 1992
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7. New Composite Biocarriers Engineered To Contain Adsorptive and Ion-Exchange Properties Improve Immobilized-Cell Bioreactor Process Dependability
Novel biocarriers that combine the adsorptive properties of activated carbon with the ion-exchange properties of zeolite-based type Z inorganic oxide biocarriers (D. R. Durham, L. C. Marshall, J. G. Miller, and A. B. Chmurny, Appl. Environ, Microbiol. 60:3329-3335, 1994.) were developed. These biocarriers, designated Type CZ, possess fundamental properties t
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8. Hydroxylation and biodegradation of 6-methylquinoline by pseudomonads in aqueous and nonaqueous immobilized-cell bioreactors.
Selective culturing of pseudomonads that could degrade quinoline led to enrichment cultures and pure cultures with expanded substrate utilization and transformation capabilities for substituted quinolines in immobilized and batch cultures. Immobilized cells of the pseudomonad cultures rapidly transformed quinolines to hydroxyquinolines in bioreactors and wer
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9. Propachlor Removal by Pseudomonas Strain GCH1 in an Immobilized-Cell System
A bacterial strain capable of growing on propachlor (2-chloro-N-isopropylacetanilide) was isolated from soil by using enrichment and isolation techniques. The strain isolated, designated GCH1, was classified as a member of the genus Pseudomonas. Washed-cell suspensions of strain GCH1 accumulated N-isopropylacetanilide, acetanilide, acetamide, and catechol. P
American Society for Microbiology.
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10. Reduction of Technetium by Desulfovibrio desulfuricans: Biocatalyst Characterization and Use in a Flowthrough Bioreactor
Resting cells of Desulfovibrio desulfuricans coupled the oxidation of a range of electron donors to Tc(VII) reduction. The reduced technetium was precipitated as an insoluble low-valence oxide. The optimum electron donor for the biotransformation was hydrogen, although rapid rates of reduction were also supported when formate or pyruvate was supplied to the
American Society for Microbiology.
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11. Spatial Distribution of Total, Ammonia-Oxidizing, and Denitrifying Bacteria in Biological Wastewater Treatment Reactors for Bioregenerative Life Support
Bioregenerative life support systems may be necessary for long-term space missions due to the high cost of lifting supplies and equipment into orbit. In this study, we investigated two biological wastewater treatment reactors designed to recover potable water for a spacefaring crew being tested at Johnson Space Center. The experiment (Lunar-Mars Life Support
American Society for Microbiology.
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12. Stabilization of microbial cytochrome P-450 activity by creation of station-phase conditions in a continuously operated immobilized-cell reactor.
Bacillus megaterium (ATCC 13368) exhibits cytochrome P-450 monooxygenase activity (referred to herein as Cyt P-450 meg) catalyzing 15 beta-steroid hydroxylation. This activity belongs to the widespread ferredoxin reductase-ferredoxin-Cyt P-450 type of monooxygenases, providing a representative model system for this type of activity. The level of Cyt P-450 me