Immunoaffinity
Mostrando 1-12 de 428 artigos, teses e dissertações.
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1. Leukoencephalomalacia in horses associated with immature corn consumption
RESUMO: A leucoencefalomalácia dos equinos (ELEM) é uma doença causada pela ingestão de micotoxinas (fumonisinas) produzidas por fungos do gênero Fusarium que infectam o milho e/ou seus subprodutos. A doença tem sido descrita pela ingestão de milho maduro com umidade acima de 15% em temperatura ambiente abaixo de 20°C. O objetivo deste trabalho foi
Cienc. Rural. Publicado em: 14/03/2019
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2. Development and characterization of an immunoaffinity column for the detection of danofloxacin residues in milk samples
Abstract In this study, an immunoaffinity column was prepared by covalently coupling of polyclonal antibody against danofloxacin (DAN) with chitosan microparticles and its potential application to selective extraction of DAN from food samples was also evaluated. The produced antibody exhibited high sensitivity, with an IC50 value of 4.56 ng/mL towards DAN,
Food Sci. Technol. Publicado em: 30/07/2018
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3. Incidence of aflatoxin M1 contamination in milk, white cheese, kashar and butter from Sakarya, Turkey
Abstract The purpose of this research is to determine the incidence and occurrence of aflatoxin M1 (AFM1) in 104 samples of milk, white cheese, kashar and butter in Sakarya, Turkey. Followed by immunoaffinity column (IAC) purification, high performance liquid chromatography (HPLC)-fluorescence detection method was implemented to analyze the samples. Results
Food Sci. Technol. Publicado em: 16/04/2018
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4. A Simple and Fast Method for Magnetic Solid Phase Extraction of Ochratoxin A
We report on the synthesis of dopamine loaded magnetic nanoparticles (MNPs) for new, simple, fast and repeatable extraction of ochratoxin A from different solvents and milk without utilizing immunoaffinity columns and even high-tech devices. To this end, Fe3O4 nanoparticles (NPs) were synthesized using thermal decomposition reaction and dopamine (DPA) was th
J. Braz. Chem. Soc.. Publicado em: 2017-06
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5. Elucidating the Mode of Action of Marine Natural Products through an Immunoaffinity Fluorescent (IAF) Approach
Understanding the target and mode of action (MOA) of small molecules activity has become a critical feature in guiding the transition between drug discovery and clinical evaluation. While classically placed at the latter stages of a discovery program, we now describe how addressing the mode of action of natural products at the early stages of a program provi
J. Braz. Chem. Soc.. Publicado em: 2016-08
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6. Comparison of High Performance Liquid Chromatography with Fluorescence Detector and with Tandem Mass Spectrometry methods for detection and quantification of Ochratoxin A in green and roasted coffee beans
Two analytical methods for the determination and confirmation of ochratoxin A (OTA) in green and roasted coffee samples were compared. Sample extraction and clean-up were based on liquid-liquid phase extraction and immunoaffinity column. The detection of OTA was carried out with the high performance liquid chromatography (HPLC) combined either with fluoresce
Braz. arch. biol. technol.. Publicado em: 2013-12
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7. Aflatoxin M1 contamination in grated parmesan cheese marketed in Rio de Janeiro - Brazil
This study aimed to determine the occurrence of AFM1 contamination in the samples of grated parmesan cheese marketed in the Metropolitan Region of Rio de Janeiro -Brazil. Thirty samples representing 10 major brands marketed in the region were analyzed by High Performance Liquid Chromatography with fluorescence detection (HPLC-FLD) after purification with imm
Braz. arch. biol. technol.. Publicado em: 26/11/2013
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8. Occurrence of aflatoxin B1 in natural products
The media claims for the consumption of natural resource-based food have gradually increased in both developing and developed countries. The interest in the safety of these products is partially due to the possible presence of toxigenic fungi acting as mycotoxin producers, such as aflatoxins produced during the secondary metabolism of Aspergillus flavus, A.
Braz. J. Microbiol.. Publicado em: 2012-12
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9. Desenvolvimento das barras imunosorventes de agitação e avaliação das técnicas extração sortiva em barra de agitação, microextração em sorvente empacotado e cromatografia líquida para análise de antidepressivos em amostras de plasma / Development of immunosorbent stir bars and evaluation of stir bar sorptive extraction, microextraction by packed sorbent and liquid chromatography for the analysis of antidepressants in plasma samples
In this work, polyclonal and monoclonal anti-fluoxetine antibodies were developed in rabbits and mice by immunization with fluoxetine-bovine albumin conjugate, respectively. The developed antibodies were characterized on the basis of the specificity against the drug by ELISA (enzyme linked immunosorbent assay) and, subsequently they were purified by labmade
Publicado em: 2010
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10. Ocorrência de Aflatoxina M1 em leite cru de três mesorregiões produtoras do Estado de São Paulo e sua correlação com parâmetros de qualidade do leite / Occurrence of aflatoxin M1 in raw milk samples from three producers mesoregions of São Paulo State and its correlation with quality parameters of milk
The AFM1 is a hydroxylated metabolite of aflatoxin B1 (AFB1), and is detected in milk, after ingestion of food/feed contaminated with B1. During the period of ten months (July/2009 to April/2010), it was evaluated: the occurrence of aflatoxin M1 (AFM1) in raw cow milk produced in 45 farms of three mesoregions of São Paulo State (Araçatuba, Bauru and Vale d
Publicado em: 2010
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11. Determinação de aflatoxina B1 em pimenta (Piper nigrum L.) e orégano (Origanum vulgare L.) por cromatografia em camada delgada e densitometria
An analytical study based on extraction with methanol-water, immunoaffinity cleanup and separation, identification and quantification of aflatoxin B1 by thin-layer chromatography,in ground black and white pepper and oregano was carried out. Validation of the applied methodology was done through accuracy and precision studies. Recoveries of aflatoxin B1 and r
Química Nova. Publicado em: 2008
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12. Anticorpo monoclonal anti-AFB1 : coluna de imunoafinidade e espectrofluorimetria para controle de qualidade de alimentos
The frequent occurrence of aflatoxin in foodstuffs causes a potential negative impact on Brazilian agribusiness. Aflatoxin has been classified in group 1 by International Association in Research on Cancer. The production of immunoreagents associated with immunochemical assay developments could allow accessible and low cost quality control aiming prevention o
Publicado em: 2007