Immunodiagnosis
Mostrando 1-12 de 116 artigos, teses e dissertações.
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1. Evaluation of an enzyme-linked immunospot assay for the immunodiagnosis of atypical spinal tuberculosis (atypical clinical presentation/atypical radiographic presentation) in China
BACKGROUND: Atypical spinal tuberculosis (TB) usually presents in a slowly indolent manner with nonspecific clinical presentations making the diagnosis a great challenge for physicians. New technologies for the detection of atypical spinal TB are urgently needed. The aim of this study was to assess the diagnostic value of an enzyme-linked immunospot (ELISPOT
Braz J Infect Dis. Publicado em: 2013-10
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2. Cryptic species of Paracoccidioides brasiliensis: impact on paracoccidioidomycosis immunodiagnosis
We aimed to evaluate whether the occurrence of cryptic species of Paracoccidioides brasiliensis, S1, PS2, PS3 and Paracoccidioides lutzii, has implications in the immunodiagnosis of paracoccidioidomycosis (PCM). Small quantities of the antigen gp43 were found in culture filtrates of P. lutzii strains and this molecule appeared to be more variable within P. l
Mem. Inst. Oswaldo Cruz. Publicado em: 2013-08
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3. Polyclonal antibodies to the putative coat protein of Citrus leprosis virus C expressed in Escherichia coli: production and use in immunodiagnosis
This work reports the in vitro expression of Citrus leprosis virus C (CiLV-C) putative coat protein (p29) and the production of a polyclonal antibody to be used as a tool for serological diagnosis of citrus leprosis. The ORF2/RNA1, corresponding to p29, was cloned in pET28a and transformed into Escherichia coli cells (BL21). Expression of p29 was induced in
Trop. plant pathol.. Publicado em: 14/05/2013
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4. Serodiagnosis of human neurocysticercosis using antigenic components of Taenia solium metacestodes derived from the unbound fraction from jacalin affinity chromatography
The aim of the present study was to analyse Taenia solium metacestode antigens that were derived from the unbound fraction of jacalin affinity chromatography and subsequent tert-octylphenoxy poly (oxyethylene) ethanol Triton X-114 (TX-114) partitioning in the diagnosis of human neurocysticercosis (NCC). Immunoassays were designed to detect T. solium-specifi
Mem. Inst. Oswaldo Cruz. Publicado em: 2013-05
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5. Isolamento e identificação de mimetopos de auto-antígenos para utilização em imunodiagnóstico da artrite idiopática juvenil
CHAPTER 2: Juvenile Idiopathic Arthritis (JIA) is a set of chronic diseases characterized by persistent inflammation of joints. "Idiopathic" means that no one knows the cause of illness and "Juvenile" in this case means that the onset of symptoms occurs before 16 years of age. The diagnosis of JIA is clinical and is based on the finding of arthritis in one o
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 30/06/2011
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6. Identification of candidate antigens from adult stages of Toxocara canis for the serodiagnosis of human toxocariasis
In the present work, we identified adult Toxocara canis antigens through sodium dodecyl sulfate-polyacrylamide gel electrophoresis for potential use in human toxocariasis immunodiagnosis. The sensitivity and specificity of several semi-purified antigens, as well as their cross-reactivity with other parasitic infections, were assessed by IgM and IgG-enzime li
Memórias do Instituto Oswaldo Cruz. Publicado em: 2011-03
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7. Anaplasma marginale infection in cattle from southwestern Amazonia.
study provides the first epidemiological data regarding infection by Anaplasma marginale in cattle reared in south-western Brazilian Amazonia. One simple procedure was adapted for the extraction of DNA from blood clots collected in seven microregions of Rondônia State and two mesoregions of Acre State. PCR method was used to asses the frequency of A. margin
Pesquisa Veteriária Barsileira. Publicado em: 2011
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8. Detection of major capsid protein of infectious myonecrosis virus in shrimps using monoclonal antibodies.
Infectious myonecrosis virus (IMNV) has been causing a progressive disease in farm-reared shrimps in Brazil and Indonesia. Immunodiagnosticmethods for IMNVdetection, although reliable, are not employed currently becausemonoclonal antibodies (MAbs) against this virus are not available. In this study, a fragment of the IMNVmajor capsid protein gene, comprising
Journal of Virological Methods. Publicado em: 2011
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9. Use of heterologous antigens for the immunodiagnosis of abdominal angiostrongyliasis by an enzyme-linked immunosorbent assay
Angiostrongylus costaricensis has a broad geographic distribution spanning from North to South America and the infections of vertebrates with this nematode can result in abdominal complications. Human infections are diagnosed by histological or serological methods because the isolation of larvae from feces is not feasible, as most parasites become trapped in
Memórias do Instituto Oswaldo Cruz. Publicado em: 2010-11
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10. Avaliação de frações antigênicas de Strongyloides venezuelensis obtidas por hidrofobicidade, no imunodiagnóstico da estrongiloidíase humana / Evaluation of antigenic fractions of Strongyloides venezuelensis obtained by hydrophobicity in the immunodiagnosis of human strongyloidiasis
A estrongiloidíase humana é uma parasitose intestinal de importância mundial, ocorre de forma assintomática em 50% dos indivíduos infectados, contudo, pode causar hiperinfecção e disseminação em pacientes imunodeprimidos. A utilização do diagnóstico preciso é de grande importância para identificação e controle desta parasitose. O emprego de a
Publicado em: 2010
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11. Immunodiagnosis potential of M. tuberculosis CFP-10 for active tuberculosis / Anáilise do potencial diagnóstico do anígeno CFP-10 expresso pelo M. tuberculosis.
The use of specific antigens of M. tuberculosis is extremely important for early diagnosis of tuberculosis and to control the spread of mycobacteria in the population. The CFP-10 is an antigen that is present in M. tuberculosis, but is absent in M. bovis of BCG and environmental mycobacteria. So, the objective of this study was to determine whether the immun
Publicado em: 2010
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12. Congenital toxoplasmosis: evaluation of serological methods for the detection of anti-Toxoplasma gondii IgM and IgA antibodies
A study was carried out to evaluate the presence of serological markers for the immunodiagnosis of the vertical transmission of toxoplasmosis. We tested the sensitivity, specificity and predictive values (positive and negative) of different serological methods for the early diagnosis of congenital toxoplasmosis. In a prospective longitudinal study, 50 infant
Memórias do Instituto Oswaldo Cruz. Publicado em: 2009-05