Immunoenzyme Technique
Mostrando 1-5 de 5 artigos, teses e dissertações.
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1. Padronização da expressão heterologa e de modelo de ensaio de atividade para a proteina quinase humana S6K / Standardization of the heterologous expression and of a model assay of activity for the human protein kinase S6K
The 70kDa ribosomal S6 protein kinase 1 (S6K1) is a phosphoprotein involved in the regulation of genes related to translational control in mammals. S6K1 shows distinct nuclear (a1) and cytoplasmic (a2) forms. Phosphorylation of the S6K1 best characterized target, the protein of the small ribosomal subunit (RPS6), has been generally associated to the selectiv
Publicado em: 2009
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2. Detection of of porcine parvovirus (PPV) in swine fetal tissues / Pesquisa da presença de parvovirus suino (PVS) em tecidos de fetos de suinos
Swine female reproductive quality is mainly measured by return to estrus, litter size, abortion, stillbirth and mummies. Porcine parvovirus (PPV) transplacental infection is associated to fetal death at different gestacional age, and mostly with fetal mummification due to abscence of fetal immunecompetence in early pregnancy. In late gestational age, immunec
Publicado em: 2008
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3. Identification and Purification of Specific Penicillium marneffei Antigens and Their Recognition by Human Immune Sera
Disseminated infection with the dimorphic pathogenic fungus Penicillium marneffei is increasingly seen among patients with AIDS in southeast Asian countries. Previous studies have demonstrated the presence of humoral immune responses to this fungus in patient sera; we have confirmed this work using sera from P. marneffei-infected patients (n = 21) to develop
American Society for Microbiology.
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4. Widespread distribution of bronchopulmonary endocrine cells immunoreactive for calcitonin in the lung of the normal adult rat.
The nature and distribution of bronchopulmonary endocrine cells immunoreactive for calcitonin was studied in normal adult Wistar albino rats by immunoenzyme histochemistry with the peroxidase-antiperoxidase technique. A widespread distribution of both solitary endocrine cells and neuroepithelial bodies immunoreactive for calcitonin was found. Many, but not a
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5. Two-colour immunoenzymatic technique using sequential staining by APAAP to evaluate two cell antigens.
AIMS: To extend the alkaline phosphatase-antialkaline phosphatase (APAAP) immunoenzyme single stain method to a more generally applicable double stain technique. This will allow two primary antibodies of the same isotype of IgG and specifically the nuclear antigen bromodeoxyuridine (BRdU) to be evaluated with a cell surface antigen identifier. METHOD: Sequen