Immunoglobulin Heavy Chains
Mostrando 1-12 de 282 artigos, teses e dissertações.
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1. Estratégias para expressão de um anticorpo anti-CD3 humanizado em células de mamífero
The development of new pharmaceuticals derived from recombinant antibodies had a significant impact in the treatment of various illnesses. However, due to the structural complexity of the immunoglobulin molecule, the production of these molecules in mammal cells is still challenging. One of the difficulties is the equimolar production of heavy and light chai
Publicado em: 2009
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2. Detection of immunoglobulin light and heavy chain genes rearrangements in cutaneous B cell lymphoproliferative infiltrates / Pesquisa do rearranjo dos genes das cadeias leve e pesada de imunoglobulina nos processos linfoproliferativos cutâneos de células B
INTRODUÇÃO: O diagnóstico diferencial dos processos linfoproliferativos cutâneos de célula B permanece um desafio para patologistas, dermatologistas, hematologistas e oncologistas, apesar dos recentes avanços imunoistoquímicos e moleculares. OBJETIVO: Este trabalho avaliou o auxílio diagnóstico e as limitações da pesquisa da clonalidade utilizando
Publicado em: 2007
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3. Comparison of different monoclonal antibodies against immunosuppressive proteins of Ascaris suum
The extract of Ascaris suum suppresses the humoral and cellular immune responses to unrelated antigens in the mouse. In order to further characterize the suppressive components of A. suum, we produced specific monoclonal antibodies which can provide an important tool for the identification of these proteins. The A. suum immunosuppressive fractions isolated b
Brazilian Journal of Medical and Biological Research. Publicado em: 2004-02
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4. Pontes dissulfeto inter cadeia de uma paraproteina M(JJO) IgG1 Glm(zax)
Plasma from a patient (JJO) with multiple myeloma showing high levels of M paraprotein was obtained from the faculdade de Medicina, Universidade Estadual de Campinas.The results of electrophoretic analysis followed by densitometry showed a compact band indicative of molecular homogeneity characteristic of patient presenting monoclonal gamopathy. Fraction A c
Publicado em: 1983
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5. Chromosomal location of the genes for human immunoglobulin heavy chains.
We have studied somatic cell hybrids between P3x63Ag8 mouse myeloma cells deficient in hypoxanthine phosphoribosyltransferase (EC 2.4.2.8) and either human peripheral lymphocytes or human lymphoblastoid or myeloma cells for the production of human immunoglobulin chains and for the expression of enzyme markers assigned to each of the different human chromosom
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6. A serum heterodimer from hagfish (Eptatretus stoutii) exhibits structural similarity and partial sequence identity with immunoglobulin.
We have isolated, characterized, and partially sequenced immunoglobulin from the most primitive extant nonvertebrate craniate, the hagfish, a jawless fish that may have diverged from the vertebrate lineage more than 500 million years ago. The 160-kDa protein, which is a minor serum component, is composed of two different heavy chains of 69 and 74 kDa and a l
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7. Expression of mu and gamma immunoglobulin heavy chains in different cells of a cloned mouse lymphoid line.
A cloned cell line derived from mouse bone marrow and transformed by Abelson virus is shown to synthesize two different heavy chains, mu and gamma 2B, in vitro. This characteristic is stable because it persists upon subcloning. Although most of the immunoglobulin-synthesizing cells produce either mu or gamma 2B heavy chains, a few cells contain both heavy ch
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8. Distribution of immunoglobulin heavy chains in diseased synovia.
Synovium from 142 patients with 12 different arthropathies was examined for the distribution of alpha, delta, gamma, and mu immunoglobulin heavy chains. A high proportion of plasma cells in the superficial subintima in all diseases reacted for alpha heavy chains. Only in rheumatoid disease did the synovium contain more than 10% of plasma cells reacting for m
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9. Sequence Relationships among the Variable Regions of Immunoglobulin Heavy Chains from Various Mammalian Species
Immunoglobulin heavy chains of myeloma proteins from dogs and cata have been subjected to automated sequence analysis. When the results were compared with human heavy-chain sequences, all the dog and cat proteins could be unequivocally assigned to the VHIII subgroup. This pattern contrasts with that in human proteins in which only 25% of all heavy chains seq
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10. Variable Region Sequences of Five Human Immunoglobulin Heavy Chains of the VHIII Subgroup: Definitive Identification of Four Heavy Chain Hypervariable Regions
The variable regions of five human immunoglobulin heavy chains of the VHIII subgroup have been totally sequenced. Three of the heavy chains belonged to the IgG class and two to the IgA class. Examination of these sequences, and comparison with additional published heavy chain sequences, showed that a total of four hypervariable regions is characteristic of h
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11. Variants of Mouse Myeloma Cells That Produce Short Immunoglobulin Heavy Chains
After mutagenesis of cultured mouse myeloma cells with ICR 191 or Melphalan, variant clones were isolated that synthesized immunoglobulin heavy chains shorter than those produced by the parent. These variants fell into two phenotypes, based on the size, serology, and pattern of assembly of the heavy chain. Variant chains of both types no longer reacted with
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12. Localization of Two Additional Hypervariable Regions in Immunoglobulin Heavy Chains
Cyanogen bromide fragments were isolated from the heavy chains of three human IgG myeloma proteins of the VHIII subgroup, sequenced by an automated method, and localized to the variable region. Inspection of these sequences, together with corresponding stretches from both human and animal proteins (studied in other laboratories) led to the detection of two a