Immunomagnetic Isolation
Mostrando 1-12 de 27 artigos, teses e dissertações.
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1. Detecção de Campylobacter jejuni em produtos de frango utilizando separação imunomagnética
RESUMO Campylobacter jejuni é o principal causador de gastroenterite bacteriana aguda, e a carne de frango tem se mostrado uma importante fonte de transmissão. Este microrganismo é de difícil isolamento e os métodos convencionais muitas vezes não são eficientes, podendo levar a resultados errôneos. O objetivo deste trabalho foi desenvolver e testar a
Arq. Bras. Med. Vet. Zootec.. Publicado em: 28/10/2019
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2. Utilization of immunomagnetic separation for detection of Salmonella in raw broiler parts
This study was conducted aiming to compare the conventional microbiological method to detect Salmonella in broiler parts with the Immunomagnetic Separation method (IMS) followed by plate isolation and also the IMS associated with Rappaport-Vassiliadis broth (RV). The IMS was performed following a pre- enrichment step in buffered peptone water. Sixty-one samp
Publicado em: 2010
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3. Isolamento e caracterização de celulas endoteliais prostaticas e a modulação do seu comportamento por celulas musculares lisas / Characterization and isolation of prostatic endothelialcells and modulation of your behavior by smooth muscle cells
Interações entre o epitélio e o mesênquima/estroma são importantes em diversos estágios da morfogênese, na diferenciação celular e na função geral de diversas glândulas. Na próstata, a função secretora do epitélio nos animais adultos é regulada por andrógenos que tem participação direta na manutenção do estado ativo da glândula. Sabe-s
Publicado em: 2007
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4. Detection of Listeria monocytogenes in foods by immunomagnetic separation.
Immunomagnetic separation with immunomagnetic beads was used to isolate strains of Listeria monocytogenes both from pure cultures and from heterogeneous suspensions. The monoclonal antibodies used recognized all six strains of serotype 4 but only one of three strains of serotype 1. Coating procedure, incubation time, and number of immunomagnetic beads influe
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5. Development of an Immunomagnetic Method for Selective Isolation of Actinobacillus pleuropneumoniae Serotype 1 from Tonsils
An immunomagnetic separation technique (IMS) for the selective isolation of Actinobacillus pleuropneumoniae serotype 1 was developed. Superparamagnetic polystyrene beads (immunomagnetic beads [IMBs]) were coated with purified rabbit immunoglobulin G specific for A. pleuropneumoniae serotype 1. The antibody concentration, the number of IMBs, the incubation ti
American Society for Microbiology.
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6. Detection of Helicobacter pylori in Fecal Samples of Gnotobiotic Mice Infected with H. pylori by an Immunomagnetic-Bead Separation Technique
By an immunomagnetic-bead (IMB) separation technique, isolation of Helicobacter pylori from gastrointestinal and fecal samples of gnotobiotic mice infected with the microorganism was tried. The isolation rate of H. pylori from stomach samples after IMB separation was not higher than that of direct culture of the samples. After IMB separation of feces, H. pyl
American Society for Microbiology.
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7. Immunomagnetic Isolation of Streptococcus suis Serotypes 2 and 1/2 from Swine Tonsils
Isolation of specific serotypes of Streptococcus suis from the tonsils, nasal cavities, and genital tract is difficult, since low-pathogenic serotypes and untypeable strains also inhabit these sites. An immunomagnetic separation (IMS) technique for the selective isolation of S. suis serotypes 2 and 1/2 was standardized. Superparamagnetic polystyrene beads (i
American Society for Microbiology.
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8. Rapid isolation of K88+ Escherichia coli by using immunomagnetic particles.
Superparamagnetic polymer particles precoated with sheep anti-mouse immunoglobulin G were coated with immunoglobulin G2 monoclonal antibodies to the K88 antigen of Escherichia coli (MAb-K88). These immunomagnetic particles (IMP) were used for isolation and identification of K88 antigen-positive (K88+) E. coli. The bacteria presenting the K88 antigen were eas
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9. Detection of Shigella dysenteriae type 1 and Shigella flexneri in feces by immunomagnetic isolation and polymerase chain reaction.
A combination of immunomagnetic separation (IMS) and a polymerase chain reaction (PCR) procedure was used for direct isolation and identification of Shigella dysenteriae type 1 and Shigella flexneri from feces. Immunomagnetic particles were coated with monoclonal antibody MASFB, which is specific for a common epitope of the O polysaccharides of S. dysenteria
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10. New Method Using Sedimentation and Immunomagnetic Separation for Isolation and Enumeration of Cryptosporidium parvum Oocysts and Giardia lamblia cysts
A new method for the isolation of Cryptosporidium parvum oocysts and Giardia lamblia cysts from biosolid samples has been developed that utilizes sedimentation and immunomagnetic separation. The method was used to recover stained cysts and oocysts (spike organisms) from primary settled sewage sludge, anaerobically digested sewage sludge, and bovine manure. R
American Society for Microbiology.
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11. Rapid and Sensitive Detection of Mycobacterium avium subsp. paratuberculosis in Bovine Milk and Feces by a Combination of Immunomagnetic Bead Separation-Conventional PCR and Real-Time PCR
Immunomagnetic bead separation coupled with bead beating and real-time PCR was found to be a very effective procedure for the isolation, separation, and detection of Mycobacterium avium subsp. paratuberculosis from milk and/or fecal samples from cattle and American bison. Samples were spiked with M. avium subsp. paratuberculosis organisms, which bound to imm
American Society for Microbiology.
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12. Isolation of a Pandemic O3:K6 Clone of a Vibrio parahaemolyticus Strain from Environmental and Clinical Sources in Thailand
Application of an immunomagnetic enrichment method selective for Vibrio parahaemolyticus serovar K6 allowed isolation of a strain belonging to the pandemic O3:K6 clone of V. parahaemolyticus from fresh shellfish not implicated in a clinical case in southern Thailand. Arbitrarily primed PCR profiles of this strain, clinical O3:K6 strains isolated from sporadi
American Society for Microbiology.