Intrinsic Labeling
Mostrando 1-12 de 44 artigos, teses e dissertações.
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1. Bioavailability of iron of the bean (Phaseolus vulgaris L.) with 59Fe intrinsically labeled. / Biodisponibilidade de ferro em feijão (Phaseolus Vulgaris L.) usando o 59Fe marcado intrinsecamente.
O feijão (Phaseolus vulgaris L.) é amplamente consumido no Brasil, especialmente no Nordeste, no qual se apresenta como componente da dieta básica regional (DBR). Constitui importante fonte de proteínas e ferro, por isso, é indicado na prevenção/tratamento da anemia, já que a deficiência de ferro é um dos problemas de maior prevalência nacional e
Publicado em: 2009
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2. Protection from myonecrosis, and expression of SERCA1 and calsequestrin in dystrophic laryngeal muscles / Musculos laringeos distroficos : proteção a mionecrose, expressao de SERCA1 e calsequestrina
Duchenne muscular dystrophy (DMD) and mdx mice, a model for DMD, is characterized by the lack of dystrophin expression and muscle fiber necrosis. Some muscle are enigmatically protected and admitted that an elevated expression of calcium-binding proteins. The intrinsic laryngeal muscles (ILMs) share many anatomical and physiological properties with extra-ocu
Publicado em: 2007
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3. Avaliação da Água boricada para aplicações oftalmológicas
Boric acid solution can be presented as non-sterile solution, whose ophthalmic application is one of several others. However, according to pharmacopoeial monographs, solutions for ophthalmic use have to be sterile, for preventing the risk of serious ophthalmic infections. In order to verify the consumption level and the product commerce, two investigation pr
Publicado em: 2005
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4. Antigenic properties and immunoelectron microscopic localization of Mycobacterium fortuitum beta-lactamase.
Mycobacterium fortuitum is a fast-growing Mycobacterium species which produces a beta-lactamase involved in the intrinsic resistance of the microorganism to beta-lactam antibiotics. An anti-beta-lactamase serum against the purified enzyme was raised in rabbits. Antibody binding was specific for native beta-lactamase, and enzyme activity was partially inhibit
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5. The Complete Set of Genes Encoding Major Intrinsic Proteins in Arabidopsis Provides a Framework for a New Nomenclature for Major Intrinsic Proteins in Plants1
Major intrinsic proteins (MIPs) facilitate the passive transport of small polar molecules across membranes. MIPs constitute a very old family of proteins and different forms have been found in all kinds of living organisms, including bacteria, fungi, animals, and plants. In the genomic sequence of Arabidopsis, we have identified 35 different MIP-encoding gen
American Society of Plant Physiologists.
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6. A plant receptor-like gene, the S-locus receptor kinase of Brassica oleracea L., encodes a functional serine/threonine kinase.
To investigate the catalytic properties of the Brassica oleracea S-locus receptor kinase (SRK), we have expressed the domain that is homologous to protein kinases as a fusion protein in Escherichia coli. Following in vivo labeling of cultures with 32P-labeled inorganic phosphate, we observed phosphorylation of the fusion protein on serine and threonine, but
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7. Molecular characterization of glycoprotein antigens on surface of Treponema pallidum: comparison with nonpathogenic Treponema phagedenis biotype Reiter.
Four glycoproteins of Treponema pallidum were identified by intrinsic [14C]glucosamine labeling. Only two glycoproteins were demonstrated in T. phagedenis biotype Reiter with the same technique. Glycoproteins of both treponemes were characterized as antigens and shown to be localized within the outer membranes of the microorganisms.
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8. Specific photoaffinity labeling of two plasma membrane polypeptides with an azido auxin
Plasma membrane vesicles were isolated from zucchini (Cucurbita pepo) hypocotyl tissue by aqueous phase partitioning and assessed for homogeneity by the use of membrane-specific enzyme assays. The highly pure (ca. 95%) plasma membrane vesicles maintained a pH differential across the membrane and accumulated a tritiated azido analogue of 3-indoleacetic acid (
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9. Geometry and orientation of neuronal processes in cat primary auditory cortex (AI) related to characteristic-frequency maps.
Microelectrode mapping and horseradish peroxidase oxidase histochemistry were combined to study the relationship between the characteristic-frequency representation and the intrinsic connectivity of the primary auditory cortex in the cat. Small extracellular iontophoretic injections of horseradish peroxidase within the characteristic-frequency map resulted i
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10. A general approach for purifying proteins encoded by cloned genes without using a functional assay: isolation of the uvrA gene product from radiolabeled maxicells.
The uvrA protein (UVRA) of E. coli has been extensively purified from a strain in which UVRA is overproduced and specifically labeled with 35S-methionine. This approximately 100-fold overproduction relative to normal strains is a result of having the uvrA gene present on a multicopy plasmid in a spr recA cell that makes defective lexA protein, the normal rep
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11. Apoptotic photoreceptor cell death in mouse models of retinitis pigmentosa.
Retinitis pigmentosa (RP) is a group of inherited human diseases in which photoreceptor degeneration leads to visual loss and eventually to blindness. Although mutations in the rhodopsin, peripherin, and cGMP phosphodiesterase genes have been identified in some forms of RP, it remains to be determined whether these mutations lead to photoreceptor cell death
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12. Recombinant human epidermal growth factor precursor is a glycosylated membrane protein with biological activity.
NIH 3T3 cells were transfected with cDNA corresponding to human kidney prepro-epidermal growth factor (preproEGF) under control of the inducible mouse metallothionein promoter. The synthesis of recombinant human EGF precursor by these cells has provided us with a model system for analysis of the structure and activity of this precursor. In transfected cells,