Intron Retention
Mostrando 1-12 de 41 artigos, teses e dissertações.
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1. Uso de técnicas computacionais no estudo da transcrição e regulação gênica em Homo sapiens e Mus musculus / Use of computational methods to study the transcription and gene regulation in Homo sapiens and Mus musculus
Genes, nucleotide sequences necessary for the synthesis of functional molecules, are transcribed and regulated by extremely complex cellular and molecular processes. To understand when and in which tissues the genes are expressed, their functional isoforms, control regions and the factors involved in gene regulation is one of major challenges of modern molec
Publicado em: 2008
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2. Analysis of genomic sequence features related to alternative splicing events (intron retention) in the human transcriptome / Análise de características das seqüencias genômicas relacionadas a eventos de splicing alternativo do tipo retenção de intron no transcriptoma humano
Most eukaryotic genes are split in exons and introns, requiring mRNA processing to remove intervening sequences and join exons (splicing). Exon/intron borders are defined by splice sites that are normally recognized with high fidelity, yielding the same processed mRNA each time. Notwithstanding such precise recognition, alternative joining of exons has been
Publicado em: 2007
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3. Caracterização molecular da proteína DdI-2 e mapeamento de seus domínios de interação com a proteína fosfatase do tipo-1 de Dictyostelium discoideum / Molecular characterization of DdI-2 protein and domain mapping of Dictyostelium discoideum protein phosphatase type-1
The serine/threonine phosphatase of type-1 (PP1) is a ubiquous enzyme in the cells and tissues from several species studied and regulates numerous processes such as intermediate metabolism, mRNA splicing, transcription, and apoptosis. PP1 holoenzymes consist of a well-conserved catalytic subunit (PP1c) and one or more variable regulatory subunits. In mammals
Publicado em: 2005
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4. Intron Retention May Regulate Expression of Epstein-Barr Virus Nuclear Antigen 3 Family Genes
The nuclear antigen 3 family genes (EBNA-3, EBNA-4, and EBNA-6) of Epstein-Barr virus (EBV) are important for EBV-induced immortalization and survival of B lymphocytes. However, little is known about how the expression of these genes is regulated. Each of the EBNA-3, EBNA-4, and EBNA-6 genes consists of two exons separated by a small intron. Reverse transcri
American Society for Microbiology.
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5. Evidence for Splice Site Pairing via Intron Definition in Schizosaccharomyces pombe
Schizosaccharomyces pombe pre-mRNAs are generally multi-intronic and share certain features with pre-mRNAs from Drosophila melanogaster, in which initial splice site pairing can occur via either exon or intron definition. Here, we present three lines of evidence suggesting that, despite these similarities, fission yeast splicing is most likely restricted to
American Society for Microbiology.
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6. Detection of exfoliated carcinoma cells in colonic luminal washings by identification of deranged patterns of expression of the CD44 gene.
AIMS: To investigate whether colonic cancer cells exfoliated into the lumen of the organ can be detected by identification of their abnormal CD44 gene products. METHODS: Exfoliated cells were obtained by centrifugation of saline wash-outs of 27 surgically resected colon specimens obtained from 15 patients with carcinoma, seven with ulcerative colitis and fiv
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7. Autoregulation of expression of the yeast Dbp2p 'DEAD-box' protein is mediated by sequences in the conserved DBP2 intron.
The human p68, Saccharomyces cerevisiae DBP2 and Schizosaccharomyces pombe dbp2 genes are closely related members of the 'DEAD-box' RNA helicase superfamily. All three genes contain an intron at a conserved site in RNA helicase motif V. The S.cerevisiae intron is unusual both for its position near the 3'-end of the open reading frame and for its size, 1001 n
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8. Comparative anatomy of the human APRT gene and enzyme: nucleotide sequence divergence and conservation of a nonrandom CpG dinucleotide arrangement.
The functional human adenine phosphoribosyltransferase (APRT) gene is less than 2.6 kilobases in length and contains five exons. The amino acid sequences of APRTs have been highly conserved throughout evolution. The human enzyme is 82%, 90%, and 40% identical to the mouse, hamster, and Escherichia coli enzymes, respectively. The promoter region of the human
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9. Selecting for Functional Alternative Splices in ESTs
The expressed sequence tag (EST) collection in dbEST provides an extensive resource for detecting alternative splicing on a genomic scale. Using genomically aligned ESTs, a computational tool (TAP) was used to identify alternative splice patterns for 6400 known human genes from the RefSeq database. With sufficient EST coverage, one or more alternatively spli
Cold Spring Harbor Laboratory Press.
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10. Intron evolution as a population-genetic process
Debate over the mechanisms responsible for the phylogenetic and genomic distribution of introns has proceeded largely without consideration of the population-genetic forces influencing the establishment and retention of novel genetic elements. However, a simple model incorporating random genetic drift and weak mutation pressure against intron-containing alle
The National Academy of Sciences.
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11. Conserved Target for Group II Intron Insertion in Relaxase Genes of Conjugative Elements of Gram-Positive Bacteria
The lactococcal group II intron Ll.ltrB interrupts the ltrB relaxase gene within a region that encodes a conserved functional domain. Nucleotides essential for the homing of Ll.ltrB into an intronless version of ltrB are found exclusively at positions required to encode amino acids broadly conserved in a family of relaxase proteins of gram-positive bacteria.
American Society for Microbiology.
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12. Regulated splicing of adenovirus type 5 E4 transcripts and regulated cytoplasmic accumulation of E4 mRNA.
The E4 gene of human type C adenoviruses has been shown previously to give rise to an array of mRNAs via differential splicing. In this study, the pattern of expression of these mRNAs during lytic infection was examined, and two distinct temporal classes were defined. mRNAs of the early class were distinguished from those of the late class by the presence, i