Intron Splice Site Primer
Mostrando 1-12 de 22 artigos, teses e dissertações.
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1. Utilização do Intron Splice Site primer EI-1 na discriminação de leveduras contaminantes do processo de fermentação alcoólica
Neste trabalho, utilizamos o Intron Splice Site primer EI-1 para a análise do perfil de amplificação de diferentes espécies de leveduras consideradas contaminantes no processo de fermentação alcoólica, originadas de uma destilaria no Estado da Paraíba na safra 2004/2005. Foram realizadas as etapas analíticas para discriminação molecular das levedu
Food Science and Technology. Publicado em: 2010-09
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2. cap20 gene pathogenicity in Colletotrichum gloeosporioides of the isolates / Gene de patogenicidade cap20 em isolados de Colletotrichum gloeosporioides
The early event for the penetration in some Colletotrichum species, iniciates with conidium adesion and germination on the surface of the host plant, producing germinative tube and then forming the apressorium, wich penetrates directly in the cuticle. Previous studies have identified in Colletotrichum gloeosporioides genes called cap, wich are only expressed
Publicado em: 2008
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3. CaracterizaÃÃo molecular (PCR) e infecÃÃo de Metarhizium anisopliae var. acridum e Metarhizium anisopliae var. anisopliae EM Zaprionus indianus / Molecular Characterization (PCR) and infection Metarhizium anisopliae var. acridum e Metarhizium anisopliae var. anisopliae EM Zaprionus indianus
The Metarhizium anisopliae var. acridum and M. anisopliae var. anisopliae strains were analysed for the pathogenicity to the fly Zaprionus indianus, using the concentrations 104, 105, 106, 107, 108 conidia/mL, considering the percentage of adultsâ emergency. In agreement with the used methodology, it was verified that both fungi strains presented action aga
Publicado em: 2006
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4. CaracterizaÃÃo molecular de espÃcies de Metarhizium e patogenicidade sobre Diatraea saccharalis / Molecular characterization of Metarhizium and pathogenicity on Diatraea saccharalis
Fifteen Metarhizium strains isolated from different areas and hosts were analysed upon genetic characteristics and 7 strains upon the pathogenicity to Diatraea saccharalis. The ITS (Internal Transcrided Spacer) molecular markers of rDNA, Intron splice site primer, RAPD and Microsatelite (SSR-Simple Sequence Repeats) were used to evaluate the genetic diversit
Publicado em: 2005
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5. Analysis of the genetic diversity using molecular markers is characteristic cytomorfological in Colletotrichum gloeosporioides / AnÃlise da diversidade genÃtica atravÃs de marcadores moleculares e caracterÃsticas citomorfolÃgicas de Colletotrichum gloeosporioides
Foram analisadas 20 linhagens de C. gloeosporioides quanto Ãs caracterÃsticas genÃticas e citomorfolÃgicas. Os marcadores moleculares, RAPD, microssatÃlites, Intron Spice Site Primer e regiÃo ITS do DNA ribossomal, foram utilizados para avaliar a diversidade genÃtica entre as linhagens. A anÃlise de agrupamento atravÃs do mÃtodo UPGMA confirmou a d
Publicado em: 2004
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6. Yeast pre-mRNA splicing requires a minimum distance between the 5' splice site and the internal branch acceptor site.
We have generated several deletions within the intron of a yeast actin gene construct which have lead to different splicing efficiencies as measured by Northern blot (RNA blot) and primer extension analyses. Our data especially demonstrate that a minimum distance from the 5' splice site to the internal branch acceptor site is required for accurate and effici
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7. Insertion of part of an intron into the 5' untranslated region of a Caenorhabditis elegans gene converts it into a trans-spliced gene.
In nematodes, the RNA products of some genes are trans-spliced to a 22-nucleotide spliced leader (SL), while the RNA products of other genes are not. In Caenorhabditis elegans, there are two SLs, SL1 and SL2, donated by two distinct small nuclear ribonucleoprotein particles in a process functionally quite similar to nuclear intron removal. We demonstrate her
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8. In vivo splicing of the premRNAs from early region 3 of adenovirus-2: the products of cleavage at the 5' splice site of the common intron.
The nuclear transcripts of the early region 3 from adenovirus-2 were studied for the presence of the cleavage products of premRNA at the 5' splice site of the first intervening sequence. Two molecules, free exon 1 and intron-exon 2-poly(A) were characterized by complementary methods including Northern blotting, RNase and S1 nuclease mapping, hybrid-selection
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9. A functional splice site in the 5' untranslated region of a zein gene.
Zein genes, the genes coding for the zein storage proteins of maize, have a unique gene structure where at least two promoters lie upstream of the coding region. Between the P1 promoter (900 base pairs upstream of the coding region) and the translation initiation AUG codon are 18 short reading frames. A discrepancy between the signals obtained by S1-mapping
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10. Accurate in vitro splicing of human beta-globin RNA.
Human beta-globin RNA transcribed from an exogenous DNA template is spliced in vitro by concentrated whole cell extracts from HeLa cells. Using the primer extension technique, we have shown that the small intervening sequence is spliced accurately and that the sequence of the product across the splice junction is identical to that of beta-globin mRNA prepare
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11. Characterization of the self-splicing products of a mobile intron from the nuclear rDNA of Physarum polycephalum.
We have characterized the splicing products formed in vitro from RNA derived from the mobile group I intron in the nuclear rDNA of Physarum polycephalum, Pp LSU 3. This intron is a close relative of the well known Tetrahymena intron Tt LSU 1, being inserted at exactly the same position in the rDNA and sharing about 90% sequence identity with Tt LSU 1 in the
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12. The RmInt1 group II intron has two different retrohoming pathways for mobility using predominantly the nascent lagging strand at DNA replication forks for priming
Sinorhizobium meliloti RmInt1 is an efficient mobile group II intron that uses an unknown reverse transcriptase priming mechanism as the intron ribonucleoprotein complex can reverse splice into DNA target substrates but cannot carry out site-specific second strand cleavage due to the lack of a C- terminal DNA endonuclease domain. We show here that, like othe
Oxford University Press.