Isoelectric Precipitation
Mostrando 1-12 de 126 artigos, teses e dissertações.
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1. Obtenção e avaliação de concentrados proteicos de corvina (Micropogonias furnieri) processados por extração química
The objective of this study was to obtain and evaluate physicochemical and functional properties of protein concentrates from Micropogonias furnieri produced by pH shifting process, using alkaline and acid solubilization followed by isoelectric precipitation of muscle proteins. The concentrates showed high protein content and the maxim solubility for the min
Química Nova. Publicado em: 2009
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2. Microalgae; Proteins; Functional properties; Chemical modifications; / Caracterização e modificações químicas da proteína da microalga spirulina (Spirulina máxima)
Maximum Spirulina is an unicellular microalga, that she has form of helical filament and that she grows in waters strongly alkaline; she belongs to the cianobacterial group, cianofíceas or algae green-blued and they have been used since times many old. The microalgae presented a biomass growth in summer of 1,07 g/L and a 23,63% of rental. Present a high con
Publicado em: 2009
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3. Study of the isoelectric precipitation of porcine insulin in aqueous solutions with carbon dioxide / Estudo da precipitação isoeletrica da insulina suina em soluções aquosas com o dioxido de carbono
Precipitation is a technique frequently employed in downstream process for the recovery of proteins from aqueous solutions. One example of such technique is the isoelectric precipitation, which consists of the adjustment of solution pH to the value where the protein has zero net charge, the isoelectric point, pI. Commonly, mineral acids and bases are used fo
Publicado em: 2007
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4. Separação de ampicilina produzida enzimaticamente por reação entre éster metílico de fenilglicina e ácido 6-aminopenicilânico.
The separation/concentration process of the products obtained from the enzymatic synthesis of ampicillin (AMP) catalyzed by immobilized penicillin G acylase was the focus of this Thesis. Hydrophobic resin adsorption and isoelectric precipitation were the processes herein evaluated. The antibiotic was produced in the solid phase, from 6-aminopenicillanic acid
Publicado em: 2003
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5. Developing an objective function to characterize the tradeoffs in salting out and the foam and droplet fractionation processes
There are many methods for separating and purifying proteins from dilute solutions, such as salting out/precipitation, adsorption/chromatography, foam fractionation, and droplet fractionation. In order to determine the optimal condition for a selected separation and purification process, an objective function is developed. The objective function consists of
Brazilian Journal of Chemical Engineering. Publicado em: 2000-06
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6. Caracterização nutricional e funcional da proteína recuperada de cefalotórax de camarão-rosa e estudo do aproveotamento do produto residual / Nutritional and functional characterization of the protein recovered from pink-shrimp cephalotorax and study of the residual product
A method for the protein recuperation from pink shrimp (Penaeus brasilensis and Penaeus palensis) cephalotorax was developed and the recuperated product and generated solid waste were characterized. The physical separation and protein insolubilization by isoelectric precipitation (pH4,5) and heating (70-75°C/5min) were the base of this method. The metho
Publicado em: 1983
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7. K99 surface antigen of Escherichia coli: antigenic characterization.
K99 prepared by acid precipitation hemagglutinated guinea pig erythrocytes, whereas K99 prepared by chromatography on diethylaminoethyl-Sephadex did not. K99 purified by either procedure hemagglutinated horse erythrocytes. K99 prepared by acid precipitation contained a second antigen not presnet in the K99 prepared by chromatography on diethylaminoethyl-Seph
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8. Precipitation of measles virus proteins by immunoglobulin G fractions containing groups of oligoclonal bands isolated from sera of patients with subacute sclerosing panencephalitis.
Groups of oligoclonal immunoglobulin G (IgG) bands were isolated from sera of patients with subacute sclerosing panencephalitis by employing preparative isoelectric focusing. Six IgG fractions containing two to three oligoclonal bands with different isoelectric points were used to precipitate the proteins from Vero cells infected with measles virus. The resu
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9. Isolation by Electrofocusing of Two Lymphocyte Mitogens Produced by Staphylococcus aureus
Staphylococcus aureus strain DA352, grown in a diffusate of Todd-Hewitt broth, produced two extracellular nondialyzable lymphocyte mitogens having isoelectric points of 5.5 to 5.7 and 8.6 to 9.0. The mitogens were separable from one another by isoelectric focusing and could be isolated free of detectable amounts of other staphylococcal products by ethanol pr
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10. Purification of glycoside hydrolases from Bacteroides fragilis.
Six glycoside hydrolases in the culture medium of Bacteroides fragilis--alpha-glucosidase, beta-glucosidase, alpha-galactosidase, beta-galactosidase, beta-N-acetylglucosaminidase, and alpha-L-fucosidase-were systematically purified by ammonium sulfate precipitation, gel filtration chromatography, and density gradient isoelectric focusing. The isoelectric foc
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11. Purification and Properties of Cytidine Deaminase from Normal and Leukemic Granulocytes
Cytidine deaminase, an enzyme that catalyses the deamination of both cytidine and its nucleoside analogues including the antineoplastic agents cytosine arabinoside (ara-C) and 5-azacytidine (5-azaC), has been partially purified from normal and leukemic human granulocytes. The purification procedure included heat precipitation at 70°C, ammonium sulfate preci
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12. Mycoplasma Phosphoenolpyruvate-Dependent Sugar Phosphotransferase System: Purification and Characterization of Enzyme I
The Mycoplasma phosphoenolpyruvate-dependent sugar phosphotransferase system consists of three components: a membrane-bound enzyme II, a soluble phosphocarrier protein (HPr), and a soluble enzyme I. The soluble enzyme I was purified by ammonium sulfate fractionation; Bio-Gel P-10 gel filtration; acid precipitation; diethylaminoethyl-Bio-Gel A; and Bio-Gel HT