Iviat In Vivo Induced Antigen Technology
Mostrando 1-5 de 5 artigos, teses e dissertações.
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1. Identification and characterization of involved molecules in the interaction of paracoccidioides brasiliensis with the host / Identificação e caracterização de moléculas envolvidas na interação de Paracoccidioides brasiliensis com o hospedeiro
Paracoccidioides brasiliensis causes paracoccidioidomycosis (PCM), a systemic mycosis presenting clinical manifestations ranging from mild to severe forms. A P. brasiliensis cDNA expression library was produced and screened with pooled sera from PCM patients adsorbed against antigens derived from in vitro-grown P. brasiliensis yeast cells. Sequencing DNA ins
Publicado em: 2009
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2. Identification and characterization of involved molecules in the interaction of paracoccidioides brasiliensis with the host / Identificação e caracterização de moléculas envolvidas na interação de Paracoccidioides brasiliensis com o hospedeiro
Paracoccidioides brasiliensis causes paracoccidioidomycosis (PCM), a systemic mycosis presenting clinical manifestations ranging from mild to severe forms. A P. brasiliensis cDNA expression library was produced and screened with pooled sera from PCM patients adsorbed against antigens derived from in vitro-grown P. brasiliensis yeast cells. Sequencing DNA ins
Publicado em: 2009
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3. Identificação de um novo Antígeno de Paracoccidioides brasiliensis (Lumazina Sintase) através da Técnica de IVIAT
Paracoccidioides brasiliensis is a thermally dimorphic fungus causing paracoccidioidomycosis (PCM), a mycosis that affects 10 million individuals in Latin America. The infection is acquired by inhaling airborne propagules produced by the fungal mycelium which transforms into the pathogenic yeast form, when at the body temperature. P brasiliensis expresses in
Publicado em: 2006
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4. Use of in vivo-induced antigen technology (IVIAT) to identify genes uniquely expressed during human infection with Vibrio cholerae
In vivo-induced antigen technology is a method to identify proteins expressed by pathogenic bacteria during human infection. Sera from 10 patients convalescing from cholera infection in Bangladesh were pooled, adsorbed against in vitro-grown El Tor Vibrio cholerae O1, and used to probe a genomic expression library in Escherichia coli constructed from El
National Academy of Sciences.
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5. Characterization and Pathogenic Significance of Vibrio vulnificus Antigens Preferentially Expressed in Septicemic Patients
Many important virulence genes of pathogenic bacteria are preferentially expressed in vivo. We used the recently developed in vivo-induced antigen technology (IVIAT) to identify Vibrio vulnificus genes induced in vivo. An expression library of V. vulnificus was screened by colony blot analysis by using pooled convalescent-phase serum that had been thoroughly
American Society for Microbiology.