Keratin Analysis
Mostrando 1-12 de 52 artigos, teses e dissertações.
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1. Preparation and characterization of electrospun human hair keratin / poly (ethylene oxide) composite nanofibers
Keratin, as one of the most abundant proteins, has been widely used for bio-related applications due to its biocompatibility and biodegradability. In this study, keratin was extracted from human hair by sulphitolysis extraction method and then blended with poly (ethylene oxide) (PEO) at different proportions. The keratin/PEO mixture was dissolved in distille
Matéria (Rio J.). Publicado em: 2014-12
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2. Keratinized versus non-keratinized preputial flap onlay urethroplasty: does it make any difference in the histological analysis? An experimental study in rabbits
Purpose To compare the histological characteristics of keratinized versus non-keratinized onlay island flaps in an experimental rabbit model. Materials and Methods Sixteen male rabbits were randomly allocated into two experimental groups: keratinized and non-keratinized onlay island flaps. A defect was created in the ventral aspect of the penile urethra. I
Int. braz j urol.. Publicado em: 2013-12
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3. Atividade enzimática de lysobacter sp. isolada de penas de pinguins da Ilha de Rei George, Antártica
Keratinolitic micro-organisms are found in diverse environments, having already been described between fungi and bacteria. Among the microbial enzymes that have been gaining prominence are the keratinases, responsible for the keratin degradation. The aim of this study is to isolate and to identify micro-organisms producing keratinases from decomposing feathe
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 29/02/2012
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4. Influência do Gene pacC na Regulação de Manosiltransferases no Dermatófito Trichophyton rubrum em Função de Variações Nutricionais e pH Ambiente. / Influence of Gene pacC in Mannosiltransferase Regulation of the Dermathophyte Trichophyton rubrum in Function to Changes by pH and Nutrient Sources.
Gene expression regulation is essential for fungi to adapt to environmental adversities, such as changes in the extracellular pH, nutrient starvation, ionic strength, and temperature. The adaptive response to ambient pH is well characterized in model fungi such as Aspergillus nidulans, and involves the signal transduction pathway consisting of the products o
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 02/12/2011
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5. Purificação e caracterização da antitrombina do plasma da serpente Bothrops jararaca (Wied, 1824) (Ophidia: Viperidae, Crotalinae) / Purification and characterization of Bothrops jararaca, antithrombin (Wied, 1824) (Ophidia: Viperidae, Crotalinae)
The aim of the present work was to characterize the seed development of Araucaria angustifolia through proteomics in order to understand the physiological and biochemical changes during this process. For that, initially, three different protein extraction methods were evaluated. The extraction based on protein solubilization in 7 M urea, 2 M thiourea, 1% dit
Publicado em: 2009
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6. Análise da expressão gênica no dermatófito Trichophyton rubrum mimetizando a infecção in vitro: pH e diferentes fontes de carbono regulando genes / Analysis of gene expression in the dermatophyte Trichophyton rubrum during the mimetic infection in vitro: pH and carboun sources are regulating genes
Dermatophytes are a group of fungi filamentous that have the ability to invade keratinized substrates, causing dermatophytosis in humans and animals and only penetrate deeper if the host is immunocompromised. Trichophyton rubrum is an anthropophilic and cosmopolitan fungi, the most common agent of superficial mycoses, which uses cell components such as prote
Publicado em: 2008
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7. Adenocarcinoma colorretal: aspectos anatomopatológicos e imuno-histoquímicos do crescimento tumoral, do citoesqueleto e de marcadores de regulação do pH intracelular / Colorectal adenocarcinoma:anatomopathological and imunohistochemical aspects of tumor growth, cytoskeleton and of intracellular pH regulator markers
The aims of this study in colorectal carcinoma were: 1) Verify the distribution of the most important anatomopathological variables, and identifying their relationship with lymph node or liver metastasis. 2) Considering the associations obtained in the first aim, a group of variables was selected to verify the prediction of lymph node or liver metastasis. 3)
Publicado em: 2008
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8. Expressão gênica diferencial das células estromais obtidas de medula óssea na presença ou ausência de célula tumoral oculta em pacientes com câncer de mama / Differential gene expression of bone marrow stromal cells from breast cancer patients in the presence or abscence of occult tumor cells
Stromal cells may influence tumor development in primary and secundary sites, however, molecular characteristics of bone marrow stromal cells from breast cancer patients are almost unknown. Our aim was to evaluate the differential gene expression of bone marrow stromal cells from breast cancer patients in the presence or abscence of occult tumor cells. Bone
Publicado em: 2006
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9. Keratin gene expression in simian virus 40-transformed human keratinocytes.
Previous reports from this laboratory indicate that cultured simian virus 40 (SV40)-transformed human keratinocytes express keratin proteins characteristic of simple epithelia that are not found in their untransformed counterparts. In this study we show by in vitro translation and RNA transfer blot analysis that the altered keratin synthesis reflects changes
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10. Monoclonal antibody analysis of keratin expression in the central nervous system.
A monoclonal antibody directed against a 65-kDa brain protein demonstrates an epitope found in keratin from human epidermis. By indirect immunofluorescence, the antibody decorates intracytoplasmic filaments in a subclass of astrocytes and Purkinje cells of adult hamster brain. Double-label immunofluorescence study using antibody to glial fibrillary acidic pr
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11. Mutational analysis of the human papillomavirus type 16 E1--E4 protein shows that the C terminus is dispensable for keratin cytoskeleton association but is involved in inducing disruption of the keratin filaments.
The function of the human papillomavirus (HPV) E4 proteins is unknown. In cultured epithelial cells the proteins associate with the keratin intermediate filaments (IFs) and, for some E4 types, e.g., HPV type 16 (HPV-16), induce collapse of the keratin networks. An N-terminal leucine-rich motif (LLXLL) is a conserved feature of many E4 proteins. In a previous
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12. Tissue-specific in vivo protein-DNA interactions at the promoter region of the Xenopus 63 kDa keratin gene during metamorphosis.
The Xenopus 63 kDa keratin gene is developmentally regulated and is expressed only in the epidermis. Full activation of the 63 kDa keratin gene requires two regulatory steps, the first independent and the second dependent on the thyroid hormone triiodothyronine (T3). Sequence analysis of a genomic clone of the 63 kDa keratin gene identified potential AP2 and