Lactacystin
Mostrando 1-12 de 88 artigos, teses e dissertações.
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1. Inibição do proteasoma aumenta o estresse oxidativo e bloqueia a resposta da NADPH oxidase a estímulos em células musculares lisas vasculares / Proteasome Inhibiton increases oxidative stress and disrupts NADPH oxidase response to stimuli in vascular smooth muscle cells
Cellular processes governing vascular Nox family NADPH oxidases in disease conditions are unclear. Since redox processes are intrinsic to cell stress response, we asked whether oxidative stress merges with other types of stress via Nox(es). We focused on proteasome inhibition as a relevant stress condition. Vascular smooth muscle cells (VSMC) incubation with
Publicado em: 2009
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2. Possível envolvimento de atividade do proteassoma na germinação induzida por etileno em embriões dormentes de girassol
Sementes recém-colhidas de girassol (Helianthus annuus L., Asteraceae) apresentam dormência fisiológica localizada no eixo embrionário, bloqueando a germinação em baixas temperaturas. Contudo, os embriões dormentes apresentam germinabilidade próxima a 100 % quando incubados na presença de etileno durante a embebição. Para verificar se determinadas
Brazilian Journal of Plant Physiology. Publicado em: 2002-08
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3. Papel dos proteassomas na interação e desenvolvimento de Leishmania chagasi em macrófagos murinos. / Role of parasite proteasomes in the infectivity and intracellular development of Leishmania chagasi in murine macrophages.
Nas células eucariotas a maioria das proteínas citoplasmáticas não são degradadas nos lisossomas, mas em organelas altamente conservadas encontradas em humanos, arquibactérias, plantas e leveduras, os proteassomas. Esta estrutura multicatalítica é constituída por componentes menores, cujo núcleo funcional é o componente 20S, que contém várias at
Publicado em: 2001
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4. A beta-lactone related to lactacystin induces neurite outgrowth in a neuroblastoma cell line and inhibits cell cycle progression in an osteosarcoma cell line.
Lactacystin, a microbial natural product, induces neurite outgrowth in Neuro 2A mouse neuroblastoma cells and inhibits progression of synchronized Neuro 2A cells and MG-63 human osteosarcoma cells beyond the G1 phase of the cell cycle. A related beta-lactone, clasto-lactacystin beta-lactone, formally the product of elimination of N-acetylcysteine from lactac
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5. Proteasome regulation of activation-induced T cell death
Lactacystin, a microbial metabolite that inhibits protease activity only in the proteasome, was used to study the role of the proteasome in the activation-induced cell death (AICD) of T cells. Lactacystin induces DNA fragmentation and apoptosis in a T cell hybridoma (DO.11.10) in a dose-dependent manner. Between 1 and 10 μM, the mildly cytotoxic lactacystin
The National Academy of Sciences of the USA.
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6. Proteasome Inhibitors Block Development of Plasmodium spp.
Proteasomes degrade most of the proteins inside eukaryotic cells, including transcription factors and regulators of cell cycle progression. Here we show that nanomolar concentrations of lactacystin, a specific irreversible inhibitor of the 20S proteasome, inhibit development of the exoerythrocytic and erythrocytic stages of the malaria parasite. Although lac
American Society for Microbiology.
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7. Tripeptidyl Peptidase II Promotes Maturation of Caspase-1 in Shigella flexneri-Induced Macrophage Apoptosis
The invasive enteropathogenic bacterium Shigella flexneri activates apoptosis in macrophages. Shigella-induced apoptosis requires caspase-1. We demonstrate here that tripeptidyl peptidase II (TPPII), a cytoplasmic, high-molecular-weight protease, participates in the apoptotic pathway triggered by Shigella. The TPPII inhibitor Ala-Ala-Phe-chloromethylketone (
American Society for Microbiology.
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8. Endosomal Dynamics of Met Determine Signaling Output
Proteasomal activity is required for Met receptor degradation after acute stimulation with hepatocyte growth factor (HGF). Inhibition of proteasomal activity with lactacystin leads to a block in the endocytic trafficking of Met such that the receptor fails to reach late endosomes/lysosomes, where degradation by acid-dependent proteases takes place (Hammond e
The American Society for Cell Biology.
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9. Requirement of ATM in Phosphorylation of the Human p53 Protein at Serine 15 following DNA Double-Strand Breaks
Microinjection of the restriction endonuclease HaeIII, which causes DNA double-strand breaks with blunt ends, induces nuclear accumulation of p53 protein in normal and xeroderma pigmentosum (XP) primary fibroblasts. In contrast, this induction of p53 accumulation is not observed in ataxia telangiectasia (AT) fibroblasts. HaeIII-induced p53 protein in normal
American Society for Microbiology.
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10. Lactacystin requires reactive oxygen species and Bax redistribution to induce mitochondria-mediated cell death
Blackwell Publishing Ltd.
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11. Degradation of the Met tyrosine kinase receptor by the ubiquitin-proteasome pathway.
The Met tyrosine kinase receptor is a widely expressed molecule which mediates pleiotropic cellular responses following activation by its ligand, hepatocyte growth factor/scatter factor (HGF/SF). In this communication we demonstrate that significant Met degradation is induced by HGF/SF and that this degradation can be blocked by lactacystin, an inhibitor of
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12. Lovastatin-mediated G1 arrest is through inhibition of the proteasome, independent of hydroxymethyl glutaryl-CoA reductase
In this paper we present the finding that lovastatin arrests cells by inhibiting the proteasome, which results in the accumulation of p21 and p27, leading to G1 arrest. Lovastatin is an inhibitor of hydroxymethyl glutaryl (HMG)-CoA reductase, the rate-limiting enzyme in cholesterol synthesis. Previously, we reported that lovastatin can be used to arrest cult
The National Academy of Sciences.