Laminarinase
Mostrando 1-12 de 20 artigos, teses e dissertações.
-
1. Production, purification and characterization of a thermostable β-1,3-glucanase (laminarinase) produced by Moniliophthora perniciosa
A enzima glucanase de Moniliophthora perniciosa foi produzida em meio líquido e purificada a partir do sobrenadante da cultura. A metodologia de superfície de resposta (MSR) foi usada para avaliar os efeitos das variáveis, incluindo indutor (extrato de levedura) e tempo de fermentação, na atividade da glucanase de M. perniciosa detectada no meio de cult
Anais da Academia Brasileira de Ciências. Publicado em: 27/05/2011
-
2. Implementação da análise de acoplamentos estatísticos e sua aplicação à família de proteínas tirosina fosfatases / Implementation of the statistical coupling analysis and its application to the Protein Tyrosine Phosphatases family.
A Análise de Acoplamentos Estatísticos é uma técnica computacional capaz de identificar resíduos importantes para a estrutura e função de proteínas em uma família por meio da quantificação de conservação posicional, correlação entre posições e identificação de grupos de resíduos correlacionados entre si. Neste trabalho, a análise de acop
Publicado em: 2009
-
3. Caracterização dos componentes extracelulares produzidos em cultura de célula de Rubus fruticosus (amora-preta) durante resposta de hipersensibilidade / Characterization of the extracellular compounds released from Rubus fruticosus (blackberry) cell during a hypersensitive response.
The plant-pathogen interactions trigger a series of signals that are not yet completely understood. One of the mechanisms is the hypersensitive response (HR), which is characterized by cell death in the infection site in order to prevent pathogen proliferation. Our previous studies with different elicitors demonstrated the correlation between the formation o
Publicado em: 2009
-
4. Purification and characterization of β-1,3-glucanases from insects / Purificação e caracterização de β-1,3-glucanases de insetos
P. americana salivary glands and T. molitor midgut epithelium actively secrete laminarinases into the midgut. The major laminarinases from P. americana (LIQ1, 42kDa and LAM_P, 45kDa), A. flavolineata (LAM_A, 45kDa) and T molitor (LAM_T, 50kDa) were purified until homogeneity. These enzymes have different specificities, action patterns and activesite catalyti
Publicado em: 2004
-
5. Purification and characterization of digestive beta-glucanases from Periplaneta americana (Dictyoptera) / Purificação e caracterização de β-glucanases digestivas de Periplaneta americana (Dictyoptera)
Periplaneta americana apresenta em seu tubo digestivo pelo menos oito atividades β-glucanásicas distintas. Destas, cinco puderam ser purificadas até a homogeneidade e parcialmente caracterizadas. LAM (Mr=46.000) apresenta atividade exclusiva sobre laminarina, é inibida por altas concentrações de substrato e gera oligossacarídeos de baixo grau de p
Publicado em: 2000
-
6. Laminarinase (beta-glucanase) activity in Bacteroides from the human colon.
Laminarin, a beta(1 leads to 3)-glucan similar to those found in plant cell walls, is fermented by some species of anaerobic bacteria from the human colon. Laminarinase (EC 3.2.1.6) and beta-glucosidase (EC 3.2.1.21) activities were determined in strains representing Bacteroides thetaiotaomicron, Bacteroides distasonis, and an unnamed deoxyribonucleic acid h
-
7. Purification of Phosphomannanase and Its Action on the Yeast Cell Wall
An improved assay for phosphomannanase (an enzyme required for the preparation of yeast protoplasts) has been developed based on the release of mannan from yeast cell walls. A procedure for the growth of Bacillus circulans on a large scale for maximal production of the enzyme is described. The culture medium containing the secreted enzyme was concentrated, a
-
8. Deletion of New Covalently Linked Cell Wall Glycoproteins Alters the Electrophoretic Mobility of Phosphorylated Wall Components of Saccharomyces cerevisiae
The incorporation of radioactive orthophosphate into the cell walls of Saccharomyces cerevisiae was studied. 33P-labeled cell walls were extensively extracted with hot sodium dodecyl sulfate (SDS). Of the remaining insoluble radioactivity more than 90% could be released by laminarinase. This radioactive material stayed in the stacking gel during SDS-polyacry
American Society for Microbiology.
-
9. Preparation and Purification of Glucanase and Chitinase from Bean Leaves 1
Glucanase (endo-β-1, 3-glucan 3-glucanohydrolase, EC 3.2.1.6, laminarinase, callase) and chitinase (poly-β-1, 4-[2-acetamido-2-deoxy] -d-glucoside glycanohydrolase, EC 3.2.1.14) were extracted from ethylene-treated bean (Phaseolus vulgaris L. cv. Red Kidney) leaves and purified on hydroxyapatite and carboxymethyl Sephadex columns. The glucanase prepared wa
-
10. Purification and Characterization of an Endo-(1,3)-β-d-Glucanase from Trichoderma longibrachiatum
A laminarinase [endo-(1,3)-β-d-glucanase] has been purified from Trichoderma longibrachiatum cultivated with d-glucose as the growth substrate. The enzyme was found to hydrolyze laminarin to oligosaccharides varying in size from glucose to pentaose and to lesser amounts of larger oligosaccharides. The enzyme was unable to cleave laminaribiose but hydrolyzed
-
11. Pseudomonas stutzeri YPL-1 Genetic Transformation and Antifungal Mechanism against Fusarium solani, an Agent of Plant Root Rot
An actively antagonistic bacterium that could be used as a biocontrol agent against Fusarium solani, which causes root rots with considerable losses in many important crops, was isolated from a ginseng rhizosphere and identified as a strain of Pseudomonas stutzeri. In several biochemical tests with culture filtrates of P. stutzeri YPL-1 and in mutational ana
-
12. Role of a Cell-Wall Glucan-degrading Enzyme in Mating of Schizophyllum commune
Mycelial enzyme extracts of Schizophyllum commune were prepared during vegetative growth matings leading to common-A and common-B heterokaryons and the dikaryon, and were examined for hydrolytic activity against an alkaliinsoluble cell-wall glucan (R-glucan) isolated from this mushroom. In extracts from several individual homokaryotic mycelia the R-glucanase