Laurdan
Mostrando 1-12 de 12 artigos, teses e dissertações.
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1. Estudos experimentais e teóricos dos espectros eletrônicos das sondas fluorescentes Prodan e Laurdan em solventes e bicamadas lipídicas / Experimental and theoretical studies of the electronic spectra of Prodan and Laurdan in solvents and lipid bilayers
As moléculas Prodan (6-propionil-2-dimetilamino-naftaleno) e Laurdan (6- dodecanoil-2-dimetilamina-naftaleno) são muito usadas como sondas fluorescentes em bicamadas lipídicas e, mais raramente, em membranas celulares. Esta tese de doutorado teve por objetivo realizar um estudo dessas sondas em diferentes ambientes, para ampliar o entendimento de suas est
Publicado em: 2010
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2. Técnicas de fluorescência no monitoramento de membranas modelo / Fluorescence techniques to monitor model membranes
Apresentamos os resultados de estudos sobre a utilização de técnicas baseadas no fenômeno de fluorescência para a investigação de processos relacionados a membranas modelo. Nessa investigação, estão envolvidas medidas de propriedades espectrais de absorção e emissão de luz por cromóforos adequados, determinação xperimental de perfis de decaim
Publicado em: 2008
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3. Physical state of bulk and protein-associated lipid in nicotinic acetylcholine receptor-rich membrane studied by laurdan generalized polarization and fluorescence energy transfer.
The spectral properties of the fluorescent probe laurdan (6-dodecanoyl-2-dimethylaminonaphthalene) were exploited to learn about the physical state of the lipids in the nicotinic acetylcholine receptor (AChR)-rich membrane and compare them with those in reconstituted liposomes prepared from lipids extracted from the native membrane and those formed with synt
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4. Probing HIV-1 Membrane Liquid Order by Laurdan Staining Reveals Producer Cell-dependent Differences*
Viruses acquire their envelope by budding from a host cell membrane, but viral lipid composition may differ from that of the budding membrane. We have previously reported that the HIV-1 membrane is highly enriched in cholesterol, sphingolipids, and other raft lipids, suggesting that the virus may bud from pre-existing or virus-induced lipid rafts. Here, we e
American Society for Biochemistry and Molecular Biology.
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5. Fluorescence generalized polarization of cell membranes: a two-photon scanning microscopy approach.
We use the lipophilic fluorescence probe Laurdan to study cell membranes. The generalized polarization (GP) of Laurdan-labeled cells contains useful information about membrane fluidity and polarity. A high GP is usually associated with low fluidity, low polarity, or high cholesterol content of the membranes, and a low GP is the opposite. We have combined the
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6. Two-photon fluorescence microscopy of laurdan generalized polarization domains in model and natural membranes.
Two-photon excitation microscopy shows coexisting regions of different generalized polarization (GP) in phospholipid vesicles, in red blood cells, in a renal tubular cell line, and in purified renal brushborder and basolateral membranes labeled with the fluorescent probe laurdan. The GP function measures the relative water content of the membrane. In the pre
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7. Laurdan solvatochromism: solvent dielectric relaxation and intramolecular excited-state reaction.
Absorption, steady-state, and time-resolved fluorescence measurements have been performed on laurdan dissolved either in white viscous apolar solvents or in ethanol as a function of temperature. The heterogeneity of the absorption spectra in white oils or in ethanol is consistent with semiempirical calculations performed previously on Prodan. From steady-sta
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8. Nicotinic acetylcholine receptor channels are influenced by the physical state of their membrane environment.
We investigated the effect of the physical state of the cell membrane on the activity of the nicotinic acetylcholine receptor (AChR) in various clonal cell lines transfected with the cDNAs of embryonic or adult AChR by measuring single-channel properties and some membrane physicochemical properties as a function of temperature. Unitary conductance and channe
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9. Physiological monitoring of optically trapped cells: assessing the effects of confinement by 1064-nm laser tweezers using microfluorometry.
We report the results of microfluorometric measurements of physiological changes in optically trapped immotile Chinese hamster ovary cells (CHOs) and motile human sperm cells under continuous-wave (CW) and pulsed-mode trapping conditions at 1064 nm. The fluorescence spectra derived from the exogenous fluorescent probes laurdan, acridine orange, propidium iod
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10. Effects of Pressure-Induced Membrane Phase Transitions on Inactivation of HorA, an ATP-Dependent Multidrug Resistance Transporter, in Lactobacillus plantarum
The effects of pressure on cultures of Lactobacillus plantarum were characterized by determination of the viability and activity of HorA, an ATP-binding cassette multidrug resistance transporter. Changes in the membrane composition of L. plantarum induced by different growth temperatures were determined. Furthermore, the effect of the growth temperature of a
American Society for Microbiology.
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11. Evidence for superlattice arrangements in fluid phosphatidylcholine/phosphatidylethanolamine bilayers.
Recently, evidence for cholesterol and phosphatidylcholine (PC) molecules to adapt superlattice arrangements in fluid lipid bilayers has been presented. Whether superlattice arrangements exist in other biologically relevant lipid membranes, such as phosphatidylethanolamine (PE)/PC, is still speculative. In this study, we have examined the physical properties
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12. Partition coefficient of a surfactant between aggregates and solution: application to the micelle-vesicle transition of egg phosphatidylcholine and octyl beta-D-glucopyranoside.
The mechanism of the solubilization of egg phosphatidylcholine containing 10% (M/M) of egg phosphatidic acid unilamellar vesicles by the nonionic detergent, octyl beta-D-glucopyranoside, has been investigated at both molecular and supramolecular levels by using fluorescence and turbidity measurements. In the lamellar region of the transition, the solubilizat