Lectin Pha
Mostrando 1-12 de 24 artigos, teses e dissertações.
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1. Cultivo de folÃculos prÃ-antrais caprinos em meio suplementado com a lectina PHA de Phaseolus vulgaris. / Cultivation of goat preantral follicles in medium supplemented with the lectin PHA from Phaseolus vulgaris.
Os caprinos sÃo considerados importantes fontes de carne, leite e pele, e, portanto, desempenham importante papel econÃmico especialmente na regiÃo nordeste do Brasil Diante disso, o desenvolvimento de sistemas de cultivo de folÃculos ovarianos pode otimizar potencial reprodutivo desta espÃcie. O objetivo deste trabalho foi avaliar o efeito da lectin PH
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 26/03/2012
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2. Screening of lectins from South American plants used as affinity ligands to purify rhEPO
Two groups of isoforms of rhEPO, at a concentration of 300 µg/ml, were tested as putative inhibitors of the lectinic hemagglutination reaction in order to obtain affinity ligand(s) for hormone purification: groups I (pI: 3.80; 3.89; 3.95; 4.07, 4.15 and 4.26) and groups II (pI: 4.15, 4.26; 4.38; 4.51; 4.72 and 4.93) Crude extracts from the vegetable materia
Brazilian Journal of Chemical Engineering. Publicado em: 2003-03
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3. Determinação do efeito mitogênico das lectinas de Cratylia mollis sobre linfócitos humanos utilizando um método colorimétrico
Lectins are proteins or glycoproteins that bind to specific saccharides. Several of them exhibit mitogenic activity on lymphocytes in vitro or are toxic in cultured cells. These properties are involved with carbohydrate binding sites. In this work it was determined the mitogenic activity of Cratylia mollis lectins (Cra-Sephadex and Cra-Iso 1) on human lympho
Publicado em: 2002
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4. Influencia das lectinas PHA, de Phaseolus vulgaris, WGA, de germe de trigo e jacalina, de Artocarpus integrifolia, no processo de reparação tecidual da pele de ratos
Wound healing is a complex and dynamic process, characterized by the integrated actions of different cells. Lectins are glycoproteins which can present several biological activities. Many of lectin activities may contribute to repair including increased production of cytokines, and activation of inflammatory cells and fibroblasts. The purpose of this work wa
Publicado em: 1998
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5. Differences in expression between two seed lectin alleles obtained from normal and lectin-deficient beans are maintained in transgenic tobacco
Using Agrobacterium-mediated transformation, two genes for phytohemagglutinin-L (PHA-L), the lectin seed protein of the common bean Phaseolus vulgaris, were stably integrated into the tobacco genome. The two alleles for PHA-L, dlec2 and pdlec2, were obtained from a normal cultivar (Greensleeves) and a lectin-deficient cultivar (Pinto) respectively. In the be
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6. Purification and Characterization of Arcelin Seed Protein from Common Bean 1
Arcelin, a seed protein originally discovered in wild bean accessions, was purified, characterized, and compared to phaseolin, the major seed protein of common bean, and to phytohemagglutinin (PHA), the major bean seed lectin. Arcelin and PHA has several characteristics in common. Both were glycoproteins having similar subunit Mr, deglycosylated Mr, and amin
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7. Lectin and immunohistochemical comparison of glycoconjugates in the conjunctiva of patients with and without exfoliation syndrome.
AIMS--The study was carried out to search for labelling similar to that of intraocular exfoliation material in the conjunctiva by light microscopy using lectin and immunohistochemistry. METHODS--Ten formalin fixed and paraffin embedded conjunctival biopsy specimens both from patients with and without exfoliation syndrome were studied with a panel of 11 lecti
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8. Targeting and release of phytohemagglutinin from the roots of bean seedlings.
Phytohemagglutinin (PHA), an abundant vacuolar seed protein of the common bean (Phaseolus vulgaris), is a tetramer of two homologous polypeptides, PHA-E and PHA-L. The roots of bean seedlings release into the culture medium a cross-reacting lectin that is most closely related to PHA-E. Reverse-transcriptase polymerase chain reaction with root mRNA as templat
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9. Lectin histochemistry of pregnant rat uterine tissues.
Glycoconjugate residues were examined in the rat uterus at days 10, 12 and 15 of pregnancy using 17 biotinylated lectins with specificities for a variety of carbohydrate moieties. A wide variety of glycoconjugate residues were detected in the cytoplasm of some antimesometrial and mesometrial decidual cells with lectins from Canavalia ensiformis (Con A), Lens
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10. A short domain of the plant vacuolar protein phytohemagglutinin targets invertase to the yeast vacuole.
Phytohemagglutinin (PHA), the seed lectin of the common bean, accumulates in protein storage vacuoles of storage parenchyma cells in cotyledons. When expressed in yeast, PHA is efficiently targeted to the yeast vacuole [Tague and Chrispeels (1987). J. Cell Biol. 105, 1971-1979]. To identify vacuolar sorting information in PHA, a series of 3' deletions of the
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11. Characterization of two Phaseolus vulgaris phytohemagglutinin genes closely linked on the chromosome.
A lambda 1059 library of Phaseolus vulgaris cv. 'Tendergreen' DNA was screened with a cloned lectin-like cDNA. Among the phages selected was clone lambda B10 containing two complete lectin genes in the same orientation approximately 4 kb apart. The DNA sequences of the lectin genes and their flanking regions have been determined and their transcriptional ini
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12. Sequential expression of protooncogenes during lectin-stimulated mitogenesis of normal human lymphocytes.
The proliferation of non-neoplastic T lymphocytes is regulated, in part, by the coordinated expression of genes encoding T-cell growth factor (interleukin 2, IL2), IL2 receptors (IL2R), and transferrin receptors (TFR). In addition to growth factors and their receptors, protooncogenes may regulate lymphocyte proliferation. We used cloned cDNAs homologous to 2