Leptospira Interrogans Hardjoprajitno
Mostrando 1-12 de 12 artigos, teses e dissertações.
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1. Controle da leptospirose em bovinos de leite com vacina autógena em Santo Antônio do Monte, Minas Gerais
Um surto de leptospirose foi observado em bovinos leiteiros em Santo Antônio do Monte, Minas Gerais. O rebanho apresentava reações positivas anti-leptospira sorovar Hardjo no teste de microaglutinação (MAT) e havia sido vacinado anteriormente com vacina experimental contendo a sorovariedade Hardjo. O MAT revelou 48,06% dos bovinos positivos para sorovar
Pesquisa Veterinária Brasileira. Publicado em: 2012-07
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2. Perfil proteico e antigênico da Leptospira interrogans sorovariedade Hardjo
Estudou-se o perfil proteico da membrana externa da Leptospira interrogans sorovariedade Hardjo, amostra hardjoprajitno, associado à resposta imune natural de bovinos infectados. Foram utilizados Triton X114 para a extração das proteínas de membrana externa e acetona para precipitá-las. As proteínas extraídas foram analisadas por SDS-PAGE e western bl
Ciência Rural. Publicado em: 2009-12
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3. Determinação do perfil protéico da membrana externa da Leptospira interrogans sorovariedade Hardjoprajitno
Estudou-se o perfil das proteínas da membrana externa (PME) da Leptospira interrogans sorovariedade Hardjoprajitno por meio da eletroforese bidimensional. Foram utilizadas técnicas de extração das PME com Triton x114 e precipitação com acetona. Os géis foram corados com nitrato de prata e as imagens analisadas para determinação da massa molecular da
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Publicado em: 2008-12
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4. Perfil protéico da membrana externa da Leptospira interrogans sorovariedade hardoprajitino.
Este trabalho teve como objetivo estudar o perfil das proteínas da membrana externa (PMEs) da Leptospira interrogans sorovariedade hardjoprajitno por meio da eletroforese uni e bidimensional e testes de Western blot. Foram utilizadas técnicas de extração da membrana externa com Triton x114 e precipitação das proteínas com acetona. As imagens foram ana
Publicado em: 2006
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5. Repetitive sequences cloned from Leptospira interrogans serovar hardjo genotype hardjoprajitno and their application to serovar identification.
We selected, from a genomic library of Leptospira interrogans serovar hardjo genotype hardjoprajitno, two probes containing repetitive sequences (pL1 and pL590). The hybridization patterns of these probes to DNA isolated from a variety of Leptospira serovars were examined and their ability to detect subtle differences at the genomic organization level was es
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6. Characterization of Leptospira isolates from serovar hardjo by ribotyping, arbitrarily primed PCR, and mapped restriction site polymorphisms.
Leptospira serovar hardjo isolates of the hardjoprajitno and hardjobovis genotypes were characterized by ribotyping, arbitrarily primed PCR (AP-PCR) fingerprinting, and the study of mapped restriction site polymorphisms (MRSPs) in rrs and rrl genes. After restriction of chromosomal DNA with BglII, EcoRI, or HindIII, each genotype was individualized with a di
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7. Identification and characterization of the protein antigens of Leptospira interrogans serovar hardjo.
We radiolabeled Leptospira proteins with [35S]methionine. Solubilized extracts of radiolabeled L. interrogans serovar hardjo strain hardjoprajitno were analyzed by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fluorography. We compared the protein profile obtained in this manner to the protein profiles of various [35S]methioni
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8. Detection and identification of Leptospira interrogans serovars by PCR coupled with restriction endonuclease analysis of amplified DNA.
Primers for PCR were selected from a sequenced fragment of clone pL590, which contains a repetitive element present in the genome of Leptospira interrogans serovar hardjo type hardjoprajitno (M. L. Pacciarini, M. L. Savio, S. Tagliabue, and C. Rossi, J. Clin. Microbiol. 30:1243-1249, 1992). A specific DNA fragment was amplified from the genomic DNAs of serov
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9. Repetitive sequence element cloned from Leptospira interrogans serovar hardjo type hardjo-bovis provides a sensitive diagnostic probe for bovine leptospirosis.
A repetitive sequence element was cloned from the primary etiological agent causing bovine leptospirosis in North America, Leptospira interrogans serovar hardjo type hardjo-bovis. This element was used to design a sensitive diagnostic probe which distinguishes hardjo-bovis from other pathogenic leptospires which commonly infect domestic animals in North Amer
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10. Protein and antigen profiles of prevalent serovars of Leptospira interrogans.
Whole-cell and detergent-soluble proteins, enriched for outer membrane antigens, of the Leptospira interrogans serovars present in commercially available pentavalent vaccines (hardjo, pomona, icterohaemorrhagiae, grippotyphosa, and canicola) were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting (immunoblotting). Pro
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11. Effect of heat or chemical treatment on leptospiral antigens.
The use of bacterins is one of the primary methods in the control of leptospirosis in domestic animals, especially cattle, swine, and dogs. Bacterins have been made using chemically treated or heat-inactivated organisms. Many of the initial studies indicated that the bacterins gave complete protection, whereas later reports found that some inactivation metho
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12. Effect of heat or formalin treatment of leptospires on antibody response detected by immunoblotting.
Leptospira interrogans serovar icterohaemorrhagiae RGA (RGA), live or heated at 56 degrees C for 15 min or treated with Formalin, was injected into rabbits to prepare hyperimmune serum. The pathogens L. interrogans serovars icterohaemorrhagiae RGA, icterohaemorrhagiae 1, canicola Moulton, grippotyphosa Andaman, hardjo Hardjoprajitno, and pomona Pomona and th