Ligand Blotting
Mostrando 1-12 de 93 artigos, teses e dissertações.
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1. Alternagin-C binding to α2β1 integrin controls matrix metalloprotease-9 and matrix metalloprotease-2 in breast tumor cells and endothelial cells
Abstract Background Matrix metalloproteinases (MMPs) are key players in tumor progression, helping tumor cells to modify their microenvironment, which allows cell migration to secondary sites. The role of integrins, adhesion receptors that connect cells to the extracellular matrix, in MMP expression and activity has been previously suggested. However, the m
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 24/05/2018
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2. Structural and functional analysis of VT1 and YP170B vitellins from the Rhabditid nematodes Oscheius tipulae and Caenorhabditis elegans. / Estudo das vitelinas VT1 e YP170B dos nematoides rabditídeos Oscheius tipulae e Caenorhabditis elegans: aspectos estruturais e funcionais.
The N-terminal region of OTI-VIT-1 was expressed and the recombinant polypeptides were purified. OTI-VIT-1 may be homologous to the vitellin YP170B from C. elegans. We identified an intron in the 5 region and two in 3region from Oti-vit-1. Monospecific antisera to PVIT1HisC confirmed that the gene Oti-vit-1 encodes VT1. The recombinant polypeptide P40-H, cor
Publicado em: 2009
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3. Identificação e caracterização de biomarcadores teciduais e sorológicos no câncer de mama por Phage Display
CHAPTER II: Breast cancer is one of the main causes of death among women, as there is no primary prevention. Early detection is the main objective aiming decrease mortality and increase survival. We used phage display technology to isolate ligand peptides to breast cancer tissues in order to select potential biomarkers for the improvement of diagnosis and tr
Publicado em: 2007
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4. Biochemical and molecular characterization of the Leishmania spp. telomerase reverse transcriptase component / Caracterização bioquimica e molecular do componente transcriptase reversa da telomerase de Leishmania spp
Telomeres are protein-DNA complexes that protect linear chromosomes from degradation, providing genomic stability. The telomeric sequences are G-rich and contain a 3 single-stranded region that protrudes toward the chromosome end. In Leishmania, the telomeric DNA is composed by the conserved 5 -TTAGGG-3 repeated sequence and it is replicated by telomerase. T
Publicado em: 2007
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5. Plasminogen interaction with Trypanosoma cruzi
The ability of Trypanosoma cruzi to interact with plasminogen, the zimogenic form of the blood serin protease plasmin, was examined. Immunohistochemistry studies revealed that both forms, epimastigotes and metacyclic trypomastigotes, were able to fix plasminogen in a lysine dependant manner. This interaction was corroborated by plasminogen activation studies
Memórias do Instituto Oswaldo Cruz. Publicado em: 2004-02
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6. Identification, purification and partial characterisation of an oligonucleotide receptor in membranes of HepG2 cells
The low and unpredictable uptake and cytosolic transfer of oligonucleotides (ODN) is a major reason for their limited benefit. Improving the ODN potential for therapy and research requires a better understanding of their receptor-mediated endocytosis. We have undertaken to identify a membrane ODN receptor on HepG2 cells by ligand blotting of cell extracts wi
Oxford University Press.
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7. A lamin B receptor in the nuclear envelope.
Using a solution binding assay, we show that purified 125I-labeled lamin B binds in a saturable and specific fashion to lamin-depleted avian erythrocyte nuclear membranes with a Kd of approximately 0.2 microM. This binding is significantly greater than the binding of 125I-labeled lamin A and is competitively inhibited by unlabeled ligand. We demonstrate that
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8. A ligand-free, soluble urokinase receptor is present in the ascitic fluid from patients with ovarian cancer.
We have identified a soluble form of the human urokinase plasminogen activator (uPA) receptor (uPAR) in the ascitic fluids from patients with ovarian cancer. After purification of uPAR from the ascitic fluids by ligand-affinity chromatography (pro-uPA Sepharose), the uPAR was initially identified by cross-linking to a radiolabeled amino-terminal fragment of
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9. Lipoprotein binding and endosomal itinerary of the low density lipoprotein receptor-related protein in rat liver.
The high affinity of 45Ca binding to the low density lipoprotein receptor (LDL-R) and the LDL-R-related protein (LRP) was utilized to study the subcellular distribution of these two proteins in rat liver. Like the LDL-R, LRP was manyfold enriched in rat liver endosomal membranes with a relative distribution in early and late endosomal compartments consistent
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10. The p53 protein is an unusually shaped tetramer that binds directly to DNA.
We have analyzed the size and structure of native immunopurified human p53 protein. By using a combination of chemical crosslinking, gel filtration chromatography, and zonal velocity gradient centrifugation, we have determined that the predominant form of p53 in such preparations is a tetramer. The behavior of purified p53 in gels and sucrose gradients impli
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11. 8-Bromo-Cyclic AMP Induces Phosphorylation of Two Sites in SRC-1 That Facilitate Ligand-Independent Activation of the Chicken Progesterone Receptor and Are Critical for Functional Cooperation between SRC-1 and CREB Binding Protein
Elevation of intracellular 8-bromo-cyclic AMP (cAMP) can activate certain steroid receptors and enhance the ligand-dependent activation of most receptors. During ligand-independent activation of the chicken progesterone receptor (cPRA) with the protein kinase A (PKA) activator, 8-bromo-cAMP, we found no alteration in cPRA phosphorylation (W. Bai, B. G. Rowan
American Society for Microbiology.
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12. Denaturation of Either Manduca sexta Aminopeptidase N or Bacillus thuringiensis Cry1A Toxins Exposes Binding Epitopes Hidden under Nondenaturing Conditions
The effect of polypeptide denaturation of Bacillus thuringiensis Cry1A toxins or purified Manduca sexta 120-kDa aminopeptidase N on the specificities of their interactions was investigated. Ligand and dot blotting experiments were conducted with 125I-labeled Cry1Ac, Cry1Ac mutant 509QNR-AAA511 (QNR-AAA), or 120-kDa aminopeptidase N as the probe. Mutant QNR-A
American Society for Microbiology.