Lipase Immobilization
Mostrando 1-12 de 42 artigos, teses e dissertações.
-
1. IMPROVING THE CATALYTIC FEATURES OF THE LIPASE FROM Rhizomucor miehei IMMOBILIZED ON CHITOSAN-BASED HYBRID MATRICES BY ALTERING THE CHEMICAL ACTIVATION CONDITIONS
The lipase from Rhizomucor miehei (RML) has been immobilized on chitosan-based hybrid (sodium alginate or carrageenan) matrices activated with glycidol (GLY), epichlorohydrin (EPI) or glutaraldehyde (GLU) groups. Then, the properties of the different biocatalysts have been evaluated and compared with the soluble RML. Thermal stability (at pH 7.0 and 60 °C)
Quím. Nova. Publicado em: 2020-09
-
2. OPTIMIZATION OF LIPASE IMMOBILIZATION ON MAGHEMITE AND ITS PHYSICO-CHEMICAL PROPERTIES
ABSTRACT Nanomaterial-based biocatalysts have emerged as current carriers suitable for enzyme immobilization. The nano-sized materials provide large surface area for enzyme attachment, thus increasing the probability for its efficient catalyst activity. By using magnetized nanomaterials, enhancement of the downstream processing is evident as it eases the imm
Braz. J. Chem. Eng.. Publicado em: 15/07/2019
-
3. Physical-Chemical Properties of the Support Immobead 150 Before and After the Immobilization Process of Lipase
We carried out the physico-chemical characterization of Immobead 150, a hydrophobic support for the immobilization of lipases. Thermomyces lanuginosus lipase (TLL) was immobilized on Immobead 150 by multipoint covalent attachment (ImmTLL) and the morphological, textural, structural, thermal, and physico-chemical properties of the support, before and after en
J. Braz. Chem. Soc.. Publicado em: 2017-08
-
4. Easy and Simple SiO2 Immobilization of Lipozyme CaLB-L: Its Use as a Catalyst in Acylation Reactions and Comparison with Other Lipases
In this study, lipase from Candida antarctica B (Lipozyme CaLB-L) was successfully immobilized on SiO2 through adsorption and used to obtain (R)-(+)-esters derived from (R,S)-1-phenylethanol. The new immobilized enzyme was compared with commercially immobilized lipases (Novozyme 435, Lipozyme 435 and Pseudomonas cepacia (PSC-II and PSD-I)). Lipozyme CaLB-L a
J. Braz. Chem. Soc.. Publicado em: 2017-07
-
5. Performance of Chitosan/Glycerol Phosphate Hydrogel as a Support for Lipase Immobilization
A biocompatible and biodegradable hybrid chitosan/glycerol phosphate hydrogel (CS-GP) was prepared and evaluated as support for enzyme immobilization. In this work a lipase sn-1,3 specific from Rhizopus oryzae was physically immobilized in CS-GP by adsorption (PA) and in CS-GP modified chemically with glutaraldehyde (CS-GP-GA) by covalent attachment (CA). Th
Mat. Res.. Publicado em: 29/06/2017
-
6. Immobilization of lipase on mesoporous molecular sieve MCM-48 obtained using ionic solid as a structure director and esterification reaction on solvent-free
Candida antarctica lipase B (CALB) is an enzyme able to catalyze chemical reaction, however when it is used as a free enzyme, it cannot be recovered from reaction medium. One of the alternatives is to immobilize the enzymes on a support which allows the maintenance of their catalytic activities. The purpose of this paper was to immobilize the CALB on MCM-48
Quím. Nova. Publicado em: 2017-04
-
7. Methacrylated Chitosan Based UV Curable Support for Enzyme Immobilization
UV curing is simple, fast and effective procedure for enzyme immobilization with minimized enzymatic activity. UV-curable methacrylated chitosan is here proposed as a support material for immobilization of lipase. The morphology of the polymeric support was characterized by scanning electron microscopy (SEM). Both covalently (CIL) and physically (PIL) immobi
Mat. Res.. Publicado em: 16/02/2017
-
8. SYNTHESIS, CHARACTERIZATION AND APPLICATION OF A POLYURETHANE-BASED SUPPORT FOR IMMOBILIZING MEMBRANE-BOUND LIPASE
Abstract This study conducted an assessment of polyurethane foams that were synthesized by one-shot process and used as a low-cost support to immobilize Mucor circinelloides URM 4182 whole-cells presenting high lipolytic activity. Polyols with different molecular weights (1100 to 6000 g mol-1) were applied to synthesize the polymer matrix, and the agitation
Braz. J. Chem. Eng.. Publicado em: 2017-01
-
9. Synthesis of structured triacylglycerols enriched in n-3 fatty acids by immobilized microbial lipase
Abstract The search for new biocatalysts has aroused great interest due to the variety of micro-organisms and their role as enzyme producers. Native lipases from Aspergillus niger and Rhizopus javanicus were used to enrich the n-3 long-chain polyunsaturated fatty acids content in the triacylglycerols of soybean oil by acidolysis with free fatty acids from sa
Braz. J. Microbiol.. Publicado em: 2016-12
-
10. The Influence of Organic Medium and Supports in the Resolution of (R,S)-Menthol Catalyzed by Lipases
In this study, various commercial and native lipases, free or immobilized, were used in the enantioselective resolution of (R,S)-menthol with vinyl acetate for the preparation of menthyl acetate. Lipase from Candida rugosa (AYL) was found to be the most appropriate. When the reaction was carried out at 35 ºC, using 40 mg of AYL and a molar ratio of 1:2 (alc
J. Braz. Chem. Soc.. Publicado em: 2016-12
-
11. NANOBIOCATALYTIC SYSTEMS BASED ON LIPASE-Fe3O4 AND CONVENTIONAL SYSTEMS FOR ISONIAZID SYNTHESIS: A COMPARATIVE STUDY
Abstract Superparamagnetic nanomaterials have attracted interest in many areas due to the high saturation magnetization and surface area. For enzyme immobilization, these properties favor the enzyme-support contact during the immobilization reaction and easy separation from the reaction mixture by use of low-cost magnetic processes. Iron oxide magnetic nanop
Braz. J. Chem. Eng.. Publicado em: 2016-09
-
12. The novel mesoporous silica aerogel modified with protic ionic liquid for lipase immobilization
Mesoporous silica supports (aerogels) were used to immobilize Burkholderia cepacia lipase (BC) by encapsulation (EN or ENIL), physical adsorption (ADS or ADSIL) and covalent binding (CB or CBIL) into or onto the aerogel modified with protic ionic liquid (PIL). Yield immobilization (Ya) and operational stability were determined by the hydrolytic reaction of o
Quím. Nova. Publicado em: 2016-05