Luvs
Mostrando 1-10 de 10 artigos, teses e dissertações.
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1. Efeito da carga dos lipídios na interação do BP100 em modelos de membrana / Charge effect of lipids in interaction of BP100 and model membranes
Antimicrobial peptides (AMPs) have been a promising alternative therapy to antibiotics. These molecules, consisting of less than 80 amino acids, with different structures and amino acids composition, have amphipathic character which gives them the ability to act on lipid bilayers. They act, by different mechanisms, over bacteria to mammal cells. The AMPs are
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 17/02/2012
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2. Purificação e caracterização de peptídeos antimicrobianos presentes na hemolinfa de Acutisoma longipes (Gonyleptidae; Opiliones). / Purification and characterization of antimicrobial peptides present in the haemolymph of Acutisoma longipes (Gonyleptidae; Opiliones).
No sistema imune de artrópodes, em contraste com o dos vertebrados, não ocorre uma resposta a antígenos por meio da produção de imunoglobulinas específicas contra agentes infecciosos. Portanto, a imunidade adaptativa está ausente, sendo que o sistema imune nesses animais baseia-se somente numa resposta inata. Os mecanismos presentes na resposta imune
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 27/04/2011
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3. Interação entre um peptídeo antimicrobiano e vesículas de fosfolipídeos / Interaction between an antimicrobial peptide and phosfolipids membranes.
Neste trabalho, estudamos a interação de um peptídeo antimicrobiano com membranas modelo, por meio de dicroísmo circular (CD), fluorescência e microscopia óptica. Tal peptídeo, chamado de híbrido, foi sintetizado como uma mistura das regiões mais ativas de dois outros peptídeos antimicrobianos, chamados de pediocina A e plantaricina 149. Esse pept�
Publicado em: 2009
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4. Influence of transbilayer area asymmetry on the morphology of large unilamellar vesicles.
The morphological consequences of differences in the monolayer surface areas of large unilamellar vesicles (LUVs) have been examined employing cryoelectron microscopy techniques. Surface area was varied by inducing net transbilayer transport of dioleoylphosphatidylglycerol (DOPG) in dioleoylphosphatidylcholine (DOPC):DOPG (9:1, mol:mol) LUVs in response to t
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5. Rotavirus Capsid Protein VP5* Permeabilizes Membranes
Proteolytic cleavage of the VP4 outer capsid spike protein into VP8* and VP5* proteins is required for rotavirus infectivity and for rotavirus-induced membrane permeability. In this study we addressed the function of the VP5* cleavage fragment in permeabilizing membranes. Expressed VP5* and truncated VP5* proteins were purified by nickel affinity chromatogra
American Society for Microbiology.
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6. Kinetics of the Interaction of myo1c with Phosphoinositides*
myo1c is a single-headed myosin that dynamically links membranes to the actin cytoskeleton. A putative pleckstrin homology domain has been identified in the myo1c tail that binds phosphoinositides and soluble inositol phosphates with high affinity. However, the kinetics of association and dissociation and the influence of phospholipid composition on the kine
American Society for Biochemistry and Molecular Biology.
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7. Structural and fusogenic properties of cationic liposomes in the presence of plasmid DNA.
The structural and fusogenic properties of large unilamellar vesicles (LUVs) composed of the cationic lipid N-[2,3-(dioleyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA) and 1,2-dioleoyl-3-phosphatidylethanotamine (DOPE) have been examined in the presence of pCMV5 plasmid and correlated with transfection potency. It is shown, employing lipid mixing fus
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8. Dehydroergosterol structural organization in aqueous medium and in a model system of membranes.
The aggregation of delta 5,7,9(11),22-ergostatetraen-3 beta-ol (dehydroergosterol or DHE), a fluorescent analog of cholesterol, was studied by photophysical techniques. It was concluded that the aqueous dispersions of DHE consist of strongly fluorescent microcrystals, and no evidence for self-quenching in micellar-type aggregates was found. The organization
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9. A general method, employing arsenazo III in liposomes, for study of calcium ionophores: results with A23187 and prostaglandins.
Multilamellar (MLV) and large unilamellar (LUV) lipid vesicles (liposomes) trap the metallochromic dye arsenazo III [2,7-bis(arsonophenylazo)-1,8-dihydroxynaphthalene-3,6-disulfonic acid ] in their aqueous compartments. When ionophore A23187 was preincorporated into either MLV or LUV above 0.001 mol%, addition of Ca to the outside of liposomes produced spect
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10. Bilayer Interactions of Indolicidin, a Small Antimicrobial Peptide Rich in Tryptophan, Proline, and Basic Amino Acids
Tryptophan, proline, and basic amino acids have all been implicated as being important in the assembly and structure of membrane proteins. Indolicidin, an antimicrobial 13-residue peptide-amide isolated from the cytoplasmic granules of bovine neutrophils, is highly enriched in these amino acids: five tryptophans, three prolines, three basic residues, and no