Mdck Cells
Mostrando 1-12 de 382 artigos, teses e dissertações.
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1. Potentially pathogenic Acanthamoeba genotype T4 isolated from dental units and emergency combination showers
BACKGROUND Acanthamoeba is the genus of free-living amoebae that is most frequently isolated in nature. To date, 20 Acanthamoeba genotypes have been described. Genotype T4 is responsible for approximately 90% of encephalitis and keratitis cases. Due to the ubiquitous presence of amoebae, isolation from environmental sources is not uncommon; to determine the
Mem. Inst. Oswaldo Cruz. Publicado em: 2017-12
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2. ADME studies and preliminary safety pharmacology of LDT5, a lead compound for the treatment of benign prostatic hyperplasia
This study aimed to estimate the absorption, distribution, metabolism and excretion (ADME) properties and safety of LDT5, a lead compound for oral treatment of benign prostatic hyperplasia that has previously been characterized as a multi-target antagonist of α1A-, α1D-adrenoceptors and 5-HT1A receptors. The preclinical characterization of this compound co
Braz J Med Biol Res. Publicado em: 24/11/2016
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3. Evaluation of Wondfo influenza A&B fast test based on immunochromatography assay for rapid diagnosis of influenza A H1N1
Influenza viruses cause significant morbidity and mortality in both children and adults during local outbreaks or epidemics. Therefore, a rapid test for influenza A&B would be useful. This study was conducted to evaluate the clinical performance of the Wondfo influenza A&B test for rapid diagnosis of influenza A H1N1 Infection. The rapid testing assay could
Braz J Infect Dis. Publicado em: 2013-04
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4. 2D map of proteins from human renal stone matrix and evaluation of their effect on oxalate induced renal tubular epithelial cell injury
PurposeProteins constitute a major portion of the organic matrix of human calcium oxalate (CaOx) renal stones and the matrix is considered to be important in stone formation and growth. The present study evaluates the effect of these proteins on oxalate injured renal epithelial cells accompanied by a 2D map of these proteins.Materials and MethodsProteins wer
Int. braz j urol.. Publicado em: 2013-01
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5. Isolation and characterization of canine parvovirus type 2c (CPV-2c) from symptomatic puppies
Canine parvovirus type 2 (CPV-2) is a leading cause of diarrhea in puppies in several parts of the world. In this study CPV-2 was detected and recovered from puppies showing clinical disease from Montevideo, Uruguay. Samples were processed and used to infect CRFK and MDCK cells in order to isolate the virus. Out of twelve, two samples were positive for CPV-2
Braz. J. Microbiol.. Publicado em: 2012-09
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6. Cytoprotective role of the aqueous extract of Terminalia chebula on renal epithelial cells
PURPOSE: Kidney stone is one of the most prevalent diseases worldwide. Calcium oxalate (CaOx) has been shown to be the main component of the majority of stones formed in the urinary system of the patients with urolithiasis. The present study evaluates the antilithiatic properties of Terminalia chebula commonly called as "harad" which is often used in ayurved
International braz j urol. Publicado em: 2012-04
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7. Cytotoxicity of Southeast Asian snake venoms
Cytotoxicity of venoms from eleven medically important snakes found in Southeast Asia (Naja kaouthia, Naja siamensis, Naja sumatrana, Ophiophagus hannah, Bungarus candidus, Bungarus fasciatus, Enhydrina schistosa, Calloselasma rhodostoma, Trimeresurus purpureomaculatus and Tropidolaemus sumatranus) was determined, based on the MTS cytotoxicity assay, which d
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2012
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8. Expression of mouse beta defensin 2 in Escherichia coli and its broad-spectrum antimicrobial activity
Mature mouse beta defensin 2 (mBD2) is a small cationic peptide with antimicrobial activity. Here we established a prokaryotic expression vector containing the cDNA of mature mBD2 fused with thioredoxin (TrxA), pET32a-mBD2. The vector was transformed into Escherichia Coli (E. coli) Rosseta-gami (2) for expression fusion protein. Under the optimization of fer
Brazilian Journal of Microbiology. Publicado em: 2011-09
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9. Mechanisms of RON-mediated epithelial-mesenchymal transition in MDCK cells through the MAPK pathway
The epithelial-mesenchymal transition (EMT) is involved in neoplastic metastasis, and the RON protein may be involved. In the present study, we determined the role and the mechanisms of action of RON in EMT in Madin-Darby canine kidney (MDCK) cells by Western blot and cell migration analysis. Activation of RON by macrophage stimulating protein (MSP) results
Brazilian Journal of Medical and Biological Research. Publicado em: 2011-07
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10. Renal- and calcium-dependent vascular effects of Polybia paulista wasp venom
In the present study, the effects of Polybia paulista venom (PPV) on renal and vascular tissues were investigated. Isolated kidneys perfused with PPV (1 and 3 μg/mL) had increased perfusion pressure, renal vascular resistance, urinary flow, and glomerular filtration rate; and reduced sodium tubular transport. Histological evaluation demonstrated deposits of
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2011
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11. Renal and vascular effects of Crotalus durissus cumanensis venom and its crotoxin fraction
In this study, we evaluated the actions of Crotalus durissus cumanensis venom (CDCmV), and its crotoxin (Crtx) fraction, on renal and vascular functions in Wistar rats. In isolated perfused kidneys, CDCmV (10 µg/mL) significantly increased the perfusion pressure (PP) from 110.7 ± 2.4 to 125.3 ± 2.8 mmHg after 30 minutes. This effect was accompanied by an
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2011
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12. AvaliaÃÃo dos efeitos renais da fraÃÃo L-aminoÃcido Oxidase isolada do veneno da serpente Bothrops marajoensis. / Assessment of renal effects of L-amino acid oxidase fraction isolated from snake Bothrops marajoensis venom.
There are about 3.000 species of snakes worldwide, but only 10 to 14% are venomous. Among South American countries, Brazil is the one with the largest number of accidents, with approximately 20.000 snakebites each year. According to the Department of Health of Brazil, the genus Bothrops are the main involved in snakebites in the country. Acute renal failure
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 03/08/2010