Metallo S Lactamase
Mostrando 1-12 de 35 artigos, teses e dissertações.
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1. Earliest identification of New Delhi metallo-β-lactamase 1 (NDM-1) in Acinetobacter pittii in Brazil
Abstract We report the occurrence in Brazil of the bla NDM-1 gene in Acinetobacter pittii, prior to the previously described first reports regarding the species Providencia rettgeri and Enterobacter hormaechei. Clinical isolates were investigated by polymerase chain reaction followed by bidirectional sequencing, and species was confirmed by 16S rDNA sequenci
Rev. Soc. Bras. Med. Trop.. Publicado em: 27/06/2019
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2. Misidentification of pan drug-resistant Klebsiella pneumoniae clinical isolates as a metallo-β-lactamase producers by the EDTA/DDST test
Braz J Infect Dis. Publicado em: 2015-02
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3. Unusual association of NDM-1 with KPC-2 and armA among Brazilian Enterobacteriaceae isolates
We report the microbiological characterization of four New Delhi metallo-β-lactamase-1 (blaNDM-1)-producing Enterobacteriaceae isolated in Rio de Janeiro, Brazil. blaNDM-1 was located on a conjugative plasmid and was associated with Klebsiella pneumoniae carbapenemase-2 (blaKPC-2) or aminoglycoside-resistance methylase (armA), a 16S rRNA methylase not previ
Braz J Med Biol Res. Publicado em: 05/12/2014
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4. Complete genome sequence of a clinical Bordetella pertussis isolate from Brazil
There has been a resurgence in the number of pertussis cases in Brazil and around the world. Here, the genome of a clinical Bordetella pertussis strain (Bz181) that was recently isolated in Brazil is reported. Analysis of the virulence-associated genes defining the pre- and post-vaccination lineages revealed the presence of the prn2-ptxS1A-fim3B-ptxP3 alleli
Mem. Inst. Oswaldo Cruz. Publicado em: 2014-11
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5. Purification of metallo-B-lactamase CcrA from Bacteroides fragilis with salting-out method
A simple and effective salting-out method was developed for the purification of the metallo-β-lactamase CcrA from Bacteroides fragilis based on the plasmid pMSZ02, in which the crude protein secreted into growth medium was precipitated by 80% sulfate saturation of the medium and purified with Q-Sepharose to offer pure CcrA with yield of 20.1 mg per litter m
Braz. arch. biol. technol.. Publicado em: 2013-10
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6. Estudo comparativo de fatores de virulência em isolados clínicos de Pseudomonas aeruginosa produtores e não produtores de metalo- ß-lactamase SPM-1
The invasion abilities, colonization and tissue damage presented by P. aeruginosa are due to the multiples virulence factors produced by the microorganism during the pathogenesis. Clinical isolates of SPM-1 metallo-β-lactamase Pseudomonas aeruginosa (SPM-1 MβL) from nosocomial infections present high rates morbidity and mortality and represent epid
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 29/05/2009
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7. Metallo-β-lactamase-producing Pseudomonas aeruginosa in two hospitals from southern Brazil
This study determined the prevalence of metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa in two hospitals located in the Southern part of Brazil and compare the performance of two different phenotypic tests. Thirty-one non-repetitive Pseudomonas aeruginosa isolates from various clinical samples from patients admitted to two hospitals located in Ri
Brazilian Journal of Infectious Diseases. Publicado em: 2009-06
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8. Metallo-β-Lactamase Producers in Environmental Microbiota: New Molecular Class B Enzyme in Janthinobacterium lividum
Eleven environmental samples from different sources were screened for the presence of metallo-β-lactamase-producing bacteria by using a selective enrichment medium containing a carbapenem antibiotic and subsequently testing each isolate for production of EDTA-inhibitable carbapenemase activity. A total of 15 metallo-β-lactamase-producing isolates, includin
American Society for Microbiology.
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9. A novel integron-like element carrying the metallo-beta-lactamase gene blaIMP.
A plasmid-mediated metallo-beta-lactamase gene was cloned from a carbapenem-resistant Serratia marcescens strain, AK9373. The metallo-beta-lactamase gene was identical to the blaIMP, and it was located in the space between an integrase-like gene and an aac(6')-Ib-like gene. The deduced amino acid sequence for the putative integrase gene showed considerable i
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10. Nucleotide and Amino Acid Sequences of the Metallo-β-Lactamase, ImiS, from Aeromonas veronii bv. sobria
The Aeromonas veronii bv. sobria metallo-β-lactamase gene, imiS, was cloned. The imiS open reading frame extends for 762 bp and encodes a protein of 254 amino acids with a secreted modified protein of 227 amino acids and a predicted pI of 8.1. To confirm the predicted sequence, purified ImiS was digested and the resulting peptides were identified, yielding
American Society for Microbiology.
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11. Virulence of Metallo-β-Lactamase-Producing Pseudomonas aeruginosa In Vitro and In Vivo
We evaluated the virulence of Pseudomonas aeruginosa carrying blaIMP, a metallo-β-lactamase gene, and the efficacy of ceftazidime, imipenem-cilastatin, and ciprofloxacin in the endogenous bacteremia model. The presence of blaIMP did not practically change the virulence of the parent strain, and ciprofloxacin was effective against infection with P. aeruginos
American Society for Microbiology.
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12. Biochemical Characterization of the FEZ-1 Metallo-β-Lactamase of Legionella gormanii ATCC 33297T Produced in Escherichia coli
The blaFEZ-1 gene coding for the metallo-β-lactamase of Legionella (Fluoribacter) gormanii ATCC 33297T was overexpressed via a T7 expression system in Escherichia coli BL21(DE3)(pLysS). The product was purified to homogeneity in two steps with a yield of 53%. The FEZ-1 metallo-β-lactamase exhibited a broad-spectrum activity profile, with a preference for c
American Society for Microbiology.