Metalloenzyme
Mostrando 1-12 de 85 artigos, teses e dissertações.
-
1. Searching for convergent evolution in manganese superoxidase dismutase using hydrophobic cluster analysis
There are numerous examples of convergent evolution in nature. Major ecological adaptations such as flight, loss of limbs in vertebrates, pesticide resistance, adaptation to a parasitic way of life, etc., have all evolved more than once, as seen by their analogous functions in separate taxa. But what about protein evolution? Does the environment have a stron
Genet. Mol. Biol.. Publicado em: 13/05/2014
-
2. O estudo da enzima deidroquinato sintase de Mycobacterium tuberculosis H37Rv como alvo para o desenvolvimento de fármacos antituberculose
Apesar da incidência per capita da tuberculose (TB) ter se mantido estável em 2005, o número de novos casos que surgem a cada ano continua a aumentar no mundo todo. De acordo com a Organização Mundial de Saúde, foram estimados 9,4 milhões de novos casos de TB em 2008, dos quais 1,4 milhões eram HIV - positivos, e com 1,8 milhões de mortes - o equiva
Publicado em: 2010
-
3. Fixação de nitrogênio: estrutura, função e modelagem bioinorgânica das nitrogenases
Biological nitrogen fixation, catalyzed by nitrogenases, contributes about half of the nitrogen needed to global agriculture. For forty years synthetic chemists and theoreticians have tried to understand and model the structure and function of this important metalloenzyme. Ten years after the first report on the crystal structure of the MoFe protein, scienti
Química Nova. Publicado em: 2003-12
-
4. Síntese e caracterização de novos compostos de coordenação de cobre (II) com ligantes não-simétricos N,O-doadores: contribuições para o sítio ativo da galactose oxidase
The reactions of four new unsymmetrical N,O-donor ligands, {H2BBPETEN= [N-(2-hydroxybenzyl) - N,N' - bis(2 methylpyridyl) -N'-(hydroxyethyl) ethylenodiamine], H3BPETEN=[N,N'- bis(2-hydroxybenzyl) -N- (2-methylpyridyl) -N'- (hydroxyethyl) ethylenodiamine], HTPETEN=[N,N,N'- tris(2-methylpyridyl) -N'- (hydroxyethyl) ethylenodiamine] and H3BIMETEN=[N,N'-(2-hydro
Química Nova. Publicado em: 2001-10
-
5. Purification and Properties of Dihydroorotase, a Zinc-Containing Metalloenzyme in Clostridium oroticum
-
6. First Detection of a Carbapenem-Hydrolyzing Metalloenzyme in an Enterobacteriaceae Isolate in France
American Society for Microbiology.
-
7. Electron transport components of the MnO2 reductase system and the location of the terminal reductase in a marine Bacillus.
The response of MnO2 reduction by uninduced and induced whole cells and cell extracts of Bacillus 29 to several electron transport inhibitors was compared. MnO2 reduction with glucose by uninduced whole cells and cell extracts was strongly inhibited at 0.1 mM dicumarol, 100 mM azide, and 8 mM cyanide but not by atebrine or carbon monoxide, suggesting the inv
-
8. RNA-Dependent DNA Polymerase (Reverse Transcriptase) from Avian Myeloblastosis Virus: A Zinc Metalloenzyme
RNA tumor viruses contain a characteristic RNA-dependent DNA polymerase (reverse transcriptase) which has been thought to be related to the induction of leukemia by this virus. A disturbance in a zinc-dependent enzyme system was first postulated to account for the demonstrated differences in zinc metabolism of normal and leukemic leukocytes [Vallee et al. in
-
9. Is the first enzyme of the shikimate pathway, 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (tyrosine sensitive), a copper metalloenzyme?
3-Deoxy-D-arabino-heptulosonate-7-phosphate synthase (tyrosine sensitive) was purified from Escherichia coli carrying the plasmid pKB45. Enzyme of high specific catalytic activity (70 mu/mg) was obtained from cells grown only to the early log phase. The purified protein contained Cu(II) and showed an absorption band at 350 nm. Metal-free, catalytically inact
-
10. Purification of properties of dihydroorotase, a zinc-containing metalloenzyme in Clostridium oroticum.
Dihydroorotase +4,5-L-dihydro-orotate amidohydrolase [EC 3.5.2.3]), which catalyzes the reversible cyclization of N-carbamyl-L-aspartate to L-dihydroorotate, has been purified from orotate-grown Clostridium oroticum. The enzyme is homogeneous when subjected to polyacrylamide gel electrophoresis and is stable at pH 7.6 in 0.3 M NaCl containing 10 muM ZnSO4. T
-
11. Cloning and sequencing of the class B beta-lactamase gene (ccrA) from Bacteroides fragilis TAL3636.
Bacteroides fragilis TAL3636 produces a class B, Zn2(+)-requiring beta-lactamase. The gene, ccrA, was cloned and expressed in Escherichia coli. The gene was sequenced and shown to share greater than 33% identity with the metalloenzyme from Bacillus cereus 569/H.
-
12. CENTA as a Chromogenic Substrate for Studying β-Lactamases
CENTA, a chromogenic cephalosporin, is readily hydrolyzed by β-lactamases of all classes except for the Aeromonas hydrophila metalloenzyme. Although it cannot practically be used for the detection of β-lactamase-producing strains on agar plates, it should be quite useful for kinetic studies and the detection of the enzymes in crude extracts and chromatogra
American Society for Microbiology.