Micro Chromatography Column
Mostrando 1-12 de 54 artigos, teses e dissertações.
-
1. Desenvolvimento de métodos analíticos para determinação de agrotóxicos em sedimentos por cromatografia gasosa monodimensional e bidimensional abrangente com micro detector de captura de elétrons
The development of analytical methods for determination of eight pesticides of different chemical classes (trichlorfon, propanil, fipronil, propiconazole, trifloxystrobin, permethrin, difenoconazole and azoxystrobin) in sediments with gas chromatography-micro-electron capture detector (GC/µECD) and comprehensive two-dimensional gas chromatography with micro
Química Nova. Publicado em: 2010
-
2. Folatos, capacidade antioxidante e trans-2-nonenal em cerveja brasileira / Folate, antioxidant capacity and E-2-nonenal in Brazilian beer
In the present study methods were adapted and applied to quantify levels of folate, antioxidant capacity assessment and E-2-nonenal quantification in Brazilian beers. 5 brands of Pilsen, non-alcoholic and Malzbier beers were used, in can and amber bottle. For determination of folates (folic acid - FA, 10-methyl-folic acid - 10Methyl, 5-methyltetrahydrofolate
Publicado em: 2010
-
3. Uso de filmes obtido pela polimerização por plasma de tetraetilortossilicato na fabricação de dispositivos miniaturizados. / Use of films obtained by tetraethoxysilane plasma polymerization for production of miniaturized devices.
Os antigos e já bem desenvolvidos dispositivos para tratamentos e/ou análise de amostras têm sido grandemente estudados para novas adaptações, devido à importância de se construir sistemas miniaturizados. A obtenção destes sistemas miniaturizados baseia-se não apenas na construção ou metodologia, mas pode depender de modificação superficial par
Publicado em: 2009
-
4. Desenvolvimento de aplicações tecnológicas da metodologia de phage display no diagnóstico do câncer de próstata
Prostate cancer is the second cause of death in men by tumor, surpassed only by lung cancer. Its occurrence is estimated to be 51 to 100,000 cases per year. The increasing incidence levels observed may be partially explained by the evolution of diagnostic methods, quality of information systems and longer life expectancy. The identification of new cancer spe
Publicado em: 2006
-
5. Nitração do fenol, um método em escala semi-micro para disciplina prática de 4 horas
A procedure for the nitration of phenol in a semi-micro scale, followed by separation of the formed orto- and para-nitrophenol isomers by column chromatography, is described. All the experiment, including determination of the melting point of the isolated products, require a period of 4 hours, and it is suitable for organic chemistry laboratory undergraduate
Química Nova. Publicado em: 2000-04
-
6. Caracterização fisico-quimica e avaliação de metilpirazinas no desenvolvimento no sabor, em dez cultivadores de cacau (Theobroma cacao L) produzidos no Estado de São Paulo
Ten coco a clones (Theobroma cacao L) were studied with respect to their physical, chemical and sensory characteristics and the behavior of some pyrazine derivatives during roasting. The cultivars were obtained from the Experimental Station at Pariquera-Áçú (S.P.), being part of the collection belonging to the Campinas Agronomy Institute. With the objecti
Publicado em: 1994
-
7. Kinetics of transcription in a minute column.
Immobilized enzymes or nucleic acids are widely used to analyze association and dissociation reactions. A pseudo-rapid kinetic method for studying transcription was developed by using an immobilized template DNA packed in a minute column, in a micro-scale liquid chromatography system. This method, which has a dead-time of only several seconds, is quick enoug
-
8. Functional expression of Arabidopsis thaliana anthranilate synthase subunit I in Escherichia coli.
Anthranilate synthase is involved in tryptophan (Trp) biosynthesis. Functional expression of subunit I from Arabidopsis (ASA1) was achieved in bacteria as a protein fused with glutathione S-transferase (GST). The active product was purified in a single step on a glutathione-Sepharose column. The Vmax (45 nmol min-1mg-1), the apparent K(M) for chorismate (180
-
9. Determination of ceftazidime in biological fluids by using high-pressure liquid chromatography.
Ceftazidime is a new beta-lactamase-stable third-generation cephalosporin with a broad spectrum of antimicrobial activity. To evaluate the biodisposition of the drug in infants and children, a rapid and simple high-pressure liquid chromatographic technique was developed. The method is useful for both serum and urine and involves methanol precipitation follow
-
10. Uridine phosphorylase from Acholeplasma laidlawii: purification and kinetic properties.
Uridine phosphorylase was purified 1,370-fold from sonicated extracts of Acholeplasma laidlawii by ammonium sulfate precipitation, DEAE-Sephadex column chromatography, hydroxylapatite chromatography, and Sephadex G-200 fractionation. The molecular weight of the enzyme as determined by gel filtration was approximately 65,000. [U-14C]ribose-1-phosphate (Rib-1-
-
11. HypB protein of Bradyrhizobium japonicum is a metal-binding GTPase capable of binding 18 divalent nickel ions per dimer.
Bradyrhizobium japonicum hypB encodes a protein containing an extremely histidine-rich region (24 histidine residues within a 39-amino-acid stretch) and guanine nucleotide-binding domains. The product of the hypB gene was overexpressed in Escherichia coli and purified by Ni(2+)-charged metal chelate affinity chromatography (MCAC) in a single step. In SDS/PAG
-
12. Phosphorylation and inactivation of glycogen synthase by phosphorylase kinase.
Skeletal muscle glycogen a4-synthase (EC 2.4.1.11) has been purified free of all synthase kinase and phosphatase activities by chromatography on a Glc-N-6-P-Sepharose affinity column and then on a phosphocellulose column. This preparation of glycogen synthase was tested as a substrate for purified skeletal muscle phosphorylase kinase (ATP:phosphorylase-b pho