Mini Tn5 Transposon
Mostrando 1-12 de 65 artigos, teses e dissertações.
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1. Obtenção de mutantes deficientes no acúmulo de PHA e construção de linhagens recombinantes para o controle da composição monomérica / Mutants deficient on polyhydroxyalkanoate (PHA) accumulation and construction of recombinants to control monomer composition on PHA
Pseudomonas putida produz PHA de cadeia média (PHAMCL) a partir de carboidratos e óleos vegetais. Genes de biossíntese de P3HB (poli-3-hidroxibutirato) de Ralstonia eutropha foram inseridos em P. putida IPT046 selvagem e mutantes PHA-. A expressão de phaC, codificador da PHA sintase, permitiu o acumulo de PHA com alto teor de 3HB, indicando que P. putida
Publicado em: 2010
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2. Avaliação do potencial de Burkholderia sacchari produzir o copolimero biodegradável poli(3-hidroxibutirato-co-3-hidroxihexanoato) [P(3HB-co-3HHX)]. / Evaluating the potential of Burkholderia sacchari to produce the biodegradable copolymer poly (3-hydroxybutirate-co-3-hydroxyhexanoate).
The ability of B. sacchari to accumulate poly-3-hydroxybutyrate-co-3-hydroxyhexanoate (P3HB-co-3HHx) from glucose and hexanoic acid was confirmed. 3HHx content was up to 2 mol% of PHA (<10% of the maximum theoretical 3HHx from the acid), indicating a substrate flexibility of B. sacchari PHA synthase, but high efficiency of hexanoate catabolic pathways. Therm
Publicado em: 2010
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3. Early colonization pattern of maize (Zea mays L. Poales, Poaceae) roots by Herbaspirillum seropedicae (Burkholderiales, Oxalobacteraceae)
The bacterium Herbaspirillum seropedicae is an endophytic diazotroph found in several plants, including economically important poaceous species. However, the mechanisms involved in the interaction between H. seropedicae and these plants are not completely characterized. We investigated the attachment of Herbaspirillum to maize roots and the invasion of the r
Genetics and Molecular Biology. Publicado em: 03/09/2008
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4. ESTUDOS GENÉTICOS SOBRE A VIRULÊNCIA DE Escherichia coli ENTEROAGREGATIVA ATÍPICA / Genetic studies on the virulence of atypical enteroaggregative Escherichia coli.
Escherichia coli enteroaggregative (EAEC) is emerging as a significant diarrheal pathogen. EAEC is defined by its characteristic stacked brick aggregative adherence (AA) pattern of adherence to HEp-2 cells. Most EAEC strains harbor a 60 to 65-MDa virulence plasmid (pAA). A 1-Kb fragment of pAA, referred to as the AA probe or CVD432, has been widely used for
Publicado em: 2006
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5. Transposon mutagenesis of marine Vibrio spp.
Coliphage P1 was used to transduce derivatives of transposons Tn5 and mini-Mu into marine Vibrio spp. Transposon Tn5 encoding tetracycline resistance (Tn5-132) was used to isolate mutants of Vibrio harveyi defective in genes for bioluminescence (lux). Insertion of transposon Tn5-132 into the lux gene region was demonstrated by intraspecific transduction with
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6. A Tn 10-lacZ-kanR-URA3 Gene Fusion Transposon for Insertion Mutagenesis and Fusion Analysis of Yeast and Bacterial Genes
We describe here a new variant of transposon Tn 10 especially adapted for transposon analysis of cloned yeast genes; it can equally well be used for analysis of prokaryotic genes. We have applied this element to analysis of the LEU2, RAD50, and CDC48 genes of Saccharomyces cerevisiae. This transposon, nicknamed mini-Tn 10-LUK, contains a lacZ gene without ef
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7. Conditionally replicating mycobacteriophages: A system for transposon delivery to Mycobacterium tuberculosis
Transposon mutagenesis provides a direct selection for mutants and is an extremely powerful technique to analyze genetic functions in a variety of prokaryotes. Transposon mutagenesis of Mycobacterium tuberculosis has been limited in part because of the inefficiency of the delivery systems. This report describes the development of conditionally replicating sh
The National Academy of Sciences of the USA.
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8. cis-trans isomerization of unsaturated fatty acids: cloning and sequencing of the cti gene from Pseudomonas putida P8.
Transposon mutants of Pseudomonas putida P8 were generated by applying a mini-Tn5 mutagenesis system. The mutants obtained were checked for their ability to tolerate increased temperatures and elevated phenol concentrations. Approximately 5,800 transposon mutants were used to generate a pool of 600 temperature-sensitive strains; one of these strains was iden
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9. Plasposons: Modular Self-Cloning Minitransposon Derivatives for Rapid Genetic Analysis of Gram-Negative Bacterial Genomes
A series of modular mini-transposon derivatives which permit the rapid cloning and mapping of the DNA flanking the minitransposon’s site of insertion has been developed. The basic plasposon, named TnMod, consists of the Tn5 inverted repeats, a conditional origin of replication, rare restriction endonuclease multiple cloning sites, and exchangeable antibiot
American Society for Microbiology.
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10. Inversions and deletions generated by a mini-gamma delta (Tn1000) transposon.
Intramolecular transposition by an engineered derivative of the transposon gamma delta (Tn1000) is described. This 1-kb element contains inverted repeats of the 40 bp of the delta end of gamma delta, bracketing a kan gene, but it contains no resolution site. Transposition was analyzed in two plasmids; one contained two contraselectable (conditional lethal) g
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11. A simple in vitro Tn7-based transposition system with low target site selectivity for genome and gene analysis
A robust Tn7-based in vitro transposition system is described that displays little target site selectivity, allowing the efficient recovery of many different transposon insertions in target DNAs ranging from small plasmids to cosmids to whole genomes. Two miniTn7 derivatives are described that are useful for the analysis of genes: one a derivative for making
Oxford University Press.
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12. Large-Scale Mutagenesis of the Yeast Genome Using a Tn7-Derived Multipurpose Transposon
We present here an unbiased and extremely versatile insertional library of yeast genomic DNA generated by in vitro mutagenesis with a multipurpose element derived from the bacterial transposon Tn7. This mini-Tn7 element has been engineered such that a single insertion can be used to generate a lacZ fusion, gene disruption, and epitope-tagged gene product. Us
Cold Spring Harbor Laboratory Press.