Minimal Genomes
Mostrando 1-12 de 98 artigos, teses e dissertações.
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1. Microcystin-XR degradation by bacteria isolated from public water supply system / Degradação da microcistina-XR por bactérias isoladas de sistema de abastecimento público de água
Microcystins are potent hepatotoxins and tumor promoters found in freshwaters that cause public health risks and thus represent a serious problem for water treatment plants. The cyanobacterium genus Microcystis is the most known toxin-producer and the most common bloom-forming in water reservoirs used for public supply. However, some bacteria are able to use
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 30/09/2011
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2. Comparative genome analysis of proteases, oligopeptide uptake and secretion systems in mycoplasma spp
Mycoplasmas are very fastidious in their nutritional requirements for in vitro growth and have limited biosynthetic capacity, a reflection of their reduced genomes. As a result, these bacteria depend upon external metabolites for nutrition and growth and have developed dependence on their hosts for survival and maintenance. Protein degradation and peptide im
Publicado em: 2010
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3. Application of a double-enrichment procedure for microsatellite isolation and the use of tailed primers for high throughput genotyping
The number of microsatellite loci and their allelic diversity contribute to increase accuracy and informativity of genetic estimates, however, the isolation of microsatellite loci is not only laborious but also quite expensive. We used (GATA)n and (GACA)n tetranucleotide probes and single- and double-enrichment hybridization to construct and screen a genomic
Genetics and Molecular Biology. Publicado em: 2007-03
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4. Determination of the Core of a Minimal Bacterial Gene Set†
The availability of a large number of complete genome sequences raises the question of how many genes are essential for cellular life. Trying to reconstruct the core of the protein-coding gene set for a hypothetical minimal bacterial cell, we have performed a computational comparative analysis of eight bacterial genomes. Six of the analyzed genomes are very
American Society for Microbiology.
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5. The genome of Mycobacterium leprae: a minimal mycobacterial gene set
Comparison of the recently sequenced genome of the leprosy-causing pathogen Mycobacterium leprae with other mycobacterial genomes reveals a drastic gene reduction and decay in M. leprae affecting many metabolic areas, exemplified by the retention of a minimal set of genes required for cell-wall biosynthesis.
BioMed Central.
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6. ESTGenes: Alternative Splicing From ESTs in Ensembl
We describe a novel algorithm for deriving the minimal set of nonredundant transcripts compatible with the splicing structure of a set of ESTs mapped on a genome. Sets of ESTs with compatible splicing are represented by a special type of graph. We describe the algorithms for building the graphs and for deriving the minimal set of transcripts from the graphs
Cold Spring Harbor Laboratory Press.
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7. Cloning and Sequencing of Defective Particles Derived from the Autonomous Parvovirus Minute Virus of Mice for the Construction of Vectors with Minimal cis-Acting Sequences
The production of wild-type-free stocks of recombinant parvovirus minute virus of mice [MVM(p)] is difficult due to the presence of homologous sequences in vector and helper genomes that cannot easily be eliminated from the overlapping coding sequences. We have therefore cloned and sequenced spontaneously occurring defective particles of MVM(p) with very sma
American Society for Microbiology.
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8. Minimal Requirement for a Lentivirus Vector Based on Human Immunodeficiency Virus Type 1
The use of human immunodeficiency virus vectors for gene therapy is hampered by concern over their safety. This concern might be ameliorated, in part, if the viral accessory genes and proteins could be eliminated from the vector genomes and particles. Here we describe a minimal vector system that is capable of transducing nondividing cells and which does not
American Society for Microbiology.
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9. Fast algorithms for large-scale genome alignment and comparison
We describe a suffix-tree algorithm that can align the entire genome sequences of eukaryotic and prokaryotic organisms with minimal use of computer time and memory. The new system, MUMmer 2, runs three times faster while using one-third as much memory as the original MUMmer system. It has been used successfully to align the entire human and mouse genomes to
Oxford University Press.
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10. Corrected Sequence of the Bacteriophage P22 Genome
We report the first accurate genome sequence for bacteriophage P22, correcting a 0.14% error rate in previously determined sequences. DNA sequencing technology is now good enough that genomes of important model systems like P22 can be sequenced with essentially 100% accuracy with minimal investment of time and resources.
American Society for Microbiology.
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11. Horizontal gene transfer among genomes: The complexity hypothesis
Increasingly, studies of genes and genomes are indicating that considerable horizontal transfer has occurred between prokaryotes. Extensive horizontal transfer has occurred for operational genes (those involved in housekeeping), whereas informational genes (those involved in transcription, translation, and related processes) are seldomly horizontally transfe
The National Academy of Sciences.
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12. Codon usage bias from tRNA's point of view: Redundancy, specialization, and efficient decoding for translation optimization
The selection-mutation-drift theory of codon usage plays a major role in the theory of molecular evolution by explaining the co-evolution of codon usage bias and tRNA content in the framework of translation optimization. Because most studies have focused only on codon usage, we analyzed the tRNA gene pool of 102 bacterial species. We show that as minimal gen
Cold Spring Harbor Laboratory Press.