Mpb83
Mostrando 1-10 de 10 artigos, teses e dissertações.
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1. Detecção de anticorpos específicos em vacas após a injeção de PPD
O diagnóstico da tuberculose bovina baseia-se na identificação da resposta imune do animal frente aos antígenos de Mycobacterium bovis. Embora o teste intradérmico comparativo cervical seja o mais empregado como primeiro teste diagnóstico, outras técnicas, como ELISA, tem sido investigadas para aumentar a detecção de animais infectados. O presente e
Pesq. Vet. Bras.. Publicado em: 2014-03
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2. Uma abordagem multidisciplinar para o diagnóstico de tuberculose bovina em um rebanho naturalmente infectado no Brasil
Um rebanho bovino naturalmente infectado por tuberculose foi analisado através de diferentes métodos diagnósticos. Um teste intradérmico simples (TIC) identificou 21 animais como positivos. Após 90 dias deste resultado, um teste intradérmico comparativo (TIC) foi aplicado nos 21 animais positivos ao TIS, além de outros 29 animais com resultados prévi
Pesq. Vet. Bras.. Publicado em: 2013-01
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3. Immunochemical Characterization of the MPB70/80 and MPB83 Proteins of Mycobacterium bovis
MPB70 and MPB80 (MPB70/80) and MPB83 are closely related antigens which are highly expressed in Mycobacterium bovis. MPB70/80 are soluble secreted antigens, while MPB83 is an exported lipoprotein associated with the bacterial surface. In the present study, these antigens had different mobilities in sodium dodecyl sulfate-polyacrylamide gel electrophoresis un
American Society for Microbiology.
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4. Association of Tuberculin-Boosted Antibody Responses with Pathology and Cell-Mediated Immunity in Cattle Vaccinated with Mycobacterium bovis BCG and Infected with M. bovis
Vaccine development and our understanding of the pathology of bovine tuberculosis in cattle would be greatly facilitated by definition of the immunological correlates of protection and/or pathology. In this study we analyzed humoral immune responses in Mycobacterium bovis BCG-vaccinated and control cattle (in particular, the relationship between the intrader
American Society for Microbiology.
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5. MPB70 and MPB83 as Indicators of Protein Localization in Mycobacterial Cells
Culture fluids after growth of Mycobacterium bovis BCG on Sauton medium contain actively secreted proteins and proteins released by bacterial lysis. BCG culture fluids and sonicates of Mycobacterium tuberculosis and Mycobacterium paratuberculosis were tested after separation by gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS
American Society for Microbiology.
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6. Vaccination of Guinea Pigs with DNA Encoding the Mycobacterial Antigen MPB83 Influences Pulmonary Pathology but Not Hematogenous Spread following Aerogenic Infection with Mycobacterium bovis
Protection of cattle against bovine tuberculosis by vaccination could be an important control strategy in countries where there is persistent Mycobacterium bovis infection in wildlife and in developing countries where it is not economical to implement a tuberculin test and slaughter control program. The main aim of such a vaccination strategy would be to red
American Society for Microbiology.
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7. Development of Diagnostic Reagents To Differentiate between Mycobacterium bovis BCG Vaccination and M. bovis Infection in Cattle
In Great Britain a recent independent scientific review for the government has concluded that the development of a cattle vaccine against Mycobacterium bovis holds the best long-term prospect for tuberculosis control in British herds. A sine qua non for vaccination is the development of a complementary diagnostic test to differentiate between vaccinated
American Society for Microbiology.
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8. Antigen Specificity in Experimental Bovine Tuberculosis
This report describes the kinetics of T-cell responses to a panel of mycobacterial antigens (PPD-M, PPD-A, ESAT-6, Ag85, 38kD, MPB64, MPB70, MPB83, hsp16.1, hsp65, and hsp70) following experimental infection of cattle with Mycobacterium bovis. Increased antigen-specific lymphocyte proliferation, gamma interferon, and interleukin-2 responses were observed in
American Society for Microbiology.
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9. Modulation of the Bovine Delayed-Type Hypersensitivity Responses to Defined Mycobacterial Antigens by a Synthetic Bacterial Lipopeptide
The use of defined protein and peptide antigens can overcome specificity limitations of purified protein derivatives in the detection of bovine tuberculosis when the antigens are used in blood-based tests. Since the use of these specific antigens as skin test reagents could have practical advantages, we investigated the potential of Mycobacterium bovis-speci
American Society for Microbiology.
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10. Antigen Recognition by Serum Antibodies in White-Tailed Deer (Odocoileus virginianus) Experimentally Infected with Mycobacterium bovis
White-tailed deer (Odocoileus virginianus) have emerged as reservoirs of bovine tuberculosis in northern America. For tuberculosis surveillance of deer, antibody-based assays are particularly attractive because deer are handled only once and immediate processing of the sample is not required. Sera collected sequentially from 25 Mycobacterium bovis-infected a
American Society for Microbiology.