Multiphoton Microscopy
Mostrando 1-12 de 25 artigos, teses e dissertações.
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1. Effects of artepillin C on model membranes displaying liquid immiscibility
It has been hypothesized that the therapeutic effects of artepillin C, a natural compound derived from Brazilian green propolis, are likely related to its partition in the lipid bilayer component of biological membranes. To test this hypothesis, we investigated the effects of the major compound of green propolis, artepillin C, on model membranes (small and g
Braz J Med Biol Res. Publicado em: 25/03/2019
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2. Optical techniques for the characterization and diagnostic of biological systems / TÃcnicas Ãpticas para caracterizaÃÃo e diagnÃstico de sistemas biolÃgicos
The linear and nonlinear optical properties of biological systems were measured and analyzed. Studies on the interaction of light with biosystems can lead to new findings and application in Biophotonics. New optical methods were introduced for the characterization and diagnostic of biological systems (amino acids and fungi). More specifically, a new variatio
Publicado em: 2008
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3. Ferramenta biofotonica integrada para manipulações e microscopias confocais / Integrates biophotonic tool for manipulations and confocal microscopies
The research in biomedical photonics is clearly evolving in the direction of the understanding of biological processes at the cell level. The spatial resolution to accomplish this task practically requires photonics tools. However, an integration of different photonic tools and a multimodal and functional approach will be necessary to access the mechanical a
Publicado em: 2007
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4. Invited Review Article: Imaging techniques for harmonic and multiphoton absorption fluorescence microscopy
We review the current state of multiphoton microscopy. In particular, the requirements and limitations associated with high-speed multiphoton imaging are considered. A description of the different scanning technologies such as line scan, multifoci approaches, multidepth microscopy, and novel detection techniques is given. The main nonlinear optical contrast
American Institute of Physics.
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5. Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation
Multicolor nonlinear microscopy of living tissue using two- and three-photon-excited intrinsic fluorescence combined with second harmonic generation by supermolecular structures produces images with the resolution and detail of standard histology without the use of exogenous stains. Imaging of intrinsic indicators within tissue, such as nicotinamide aden
National Academy of Sciences.
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6. Multiphoton excitation fluorescence microscopy and spectroscopy of in vivo human skin.
Multiphoton excitation microscopy at 730 nm and 960 nm was used to image in vivo human skin autofluorescence from the surface to a depth of approximately 200 microm. The emission spectra and fluorescence lifetime images were obtained at selected locations near the surface (0-50 microm) and at deeper depths (100-150 microm) for both excitation wavelengths. Ce
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7. Gene inactivation by multiphoton-targeted photochemistry
Multiphoton-targeted photochemistry was used to selectively inactivate the expression of genes in vertebrate cells. A membrane permeable DNA-associating vital dye, ethidium bromide monoacetate (visible wavelength single photon absorption peak at 530 nm) was used to photosensitize chromosomes in dividing cells. A 100-ps infrared laser beam operating at 1.06 m
The National Academy of Sciences.
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8. Improved Model of Fluorescence Recovery Expands the Application of Multiphoton Fluorescence Recovery after Photobleaching in Vivo
Multiphoton fluorescence recovery after photobleaching is a well-established microscopy technique used to measure the diffusion of macromolecules in biological systems. We have developed an improved model of the fluorescence recovery that includes the effects of convective flows within a system. We demonstrate the validity of this two-component diffusion-con
The Biophysical Society.
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9. Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence
Multiphoton microscopy relies on nonlinear light–matter interactions to provide contrast and optical sectioning capability for high-resolution imaging. Most multiphoton microscopy studies in biological systems have relied on two-photon excited fluorescence (TPEF) to produce images. With increasing applications of multiphoton microscopy to thick-tissue “i
National Academy of Sciences.
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10. Four-dimensional multiphoton imaging of brain entry, amyloid binding, and clearance of an amyloid-β ligand in transgenic mice
The lack of a specific biomarker makes preclinical diagnosis of Alzheimer's disease (AD) impossible, and it precludes assessment of therapies aimed at preventing or reversing the course of the disease. The development of a tool that enables direct, quantitative detection of the amyloid-β deposits found in the disease would provide an excellent biomarker. Th
National Academy of Sciences.
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11. Multimodal optical workstation for simultaneous linear, nonlinear microscopy and nanomanipulation: Upgrading a commercial confocal inverted microscope
In this work we propose and build a multimodal optical workstation that extends a commercially available confocal microscope (Nikon Confocal C1-Si) to include nonlinear∕multiphoton microscopy and optical manipulation∕stimulation tools such as nanosurgery. The setup allows both subsystems (confocal and nonlinear) to work independently and simultaneously.
American Institute of Physics.
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12. Multiphoton fluorescence excitation: new spectral windows for biological nonlinear microscopy.
Intrinsic, three-dimensionally resolved, microscopic imaging of dynamical structures and biochemical processes in living preparations has been realized by nonlinear laser scanning fluorescence microscopy. The search for useful two-photon and three-photon excitation spectra, motivated by the emergence of nonlinear microscopy as a powerful biophysical instrume