Naked Eye Detection
Mostrando 1-12 de 23 artigos, teses e dissertações.
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1. A Highly Selective Colorimetric Sensor for Cysteine Detection
Introducing a hybrid xanthene as a fluorophore, an ‘ensemble’-based fluorescent sensor (E)-2-(6-(diethylamino)-2-((2-hydroxyphenylimino)methyl)-3-oxo-3H-xanthen-9-yl)benzoic acid (a) was designed and synthesised for detection of cysteine. Cysteine can release CuII ion from the non-fluorescent a-CuII complex. Then hydrolytic cleavage of Schiff base a prod
J. Braz. Chem. Soc.. Publicado em: 2020-02
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2. Synthesis and Photophysical Characterization of Proton Transfer-Based Thiourea Derivatives: Potential Application as Colorimetric Naked-Eye Chemosensor for Fluoride Detection in Solution
Two new thiourea derivatives were synthesized through the reaction of photoactive aminohydroxybenzazoles and p-isothiocyanate benzoic acid via nucleophilic addition reaction. The compounds were characterized using high resolution mass spectrometry with eletrospray ionization (HRMS-ESI), Fourier transform infrared (FTIR), 13C and 1H nuclear magnetic resonance
J. Braz. Chem. Soc.. Publicado em: 2017-09
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3. LAMP technology: Rapid identification of Brucella and Mycobacterium avium subsp. paratuberculosis
In this study, we developed new sets of primers to detect Brucella spp. and M. avium subsp. paratuberculosis (MAP) through isothermal amplification. We selected a previously well-characterized target gene, bscp31, specific for Brucella spp. and IS900 for MAP. The limits of detection using the loop-mediated isothermal amplification (LAMP) protocols described
Braz. J. Microbiol.. Publicado em: 2015-06
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4. Detection of anti-cardiolipin antibodies from real sera using polymerized liposomes / Detecção de anticorpos anti-cardiolipidina em soros reais utilizando lipossomas polimerizados
This work studies the detection of anticardiolipin IgG present in the sera o patients with autoimmune diseases , using polymerized liposomes. The advantage of this system is the molecular recognition and transduction of signal in a single step. The use of real sera, instead reference sera, aimed to evaluate the potentiality of polymerized liposomes to applic
Publicado em: 2007
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5. Detecção de analitos neutros e aniônicos através do uso de sensores cromogênicos baseados na competição de uma merocianina solvatocrômica e do substrato por uma unidade receptora
Este trabalho apresenta o desenvolvimento de sensores cromogênicos para espécies neutras e aniônicas, através da interação uma merocianina solvatocrômica (merocianina de Brooker, MB) com diferentes receptores (α-, β-, γ-, hidroxipropil-β, metil-β-ciclodextrina e ácido fenil borônico). A interação não covalente de ciclodex
Publicado em: 2007
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6. Caracterização morfológica, funcional e de ocorrência das tonsilas linguais laterais / Morphology characterization, function and occurrence of the lateral lingual tonsils
This study aimed to characterize the lateral lingual tonsils as to their morphology, function and occurrence. The experimental group I was composed of 25 specimens of human tongues fixated and kept in 10% formalin until processing. The specimens were radiographed for detection of any mineralized tissue. The specimens were examined by naked eye and with aid o
Publicado em: 2007
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7. Whole-Blood Agglutination Assay for On-Site Detection of Human Immunodeficiency Virus Infection
Simple and rapid diagnostic tests are needed to curtail human immunodeficiency virus (HIV) infection, especially in the developing and underdeveloped nations of the world. The visible-agglutination assay for the detection of HIV with the naked eye (NEVA HIV, which represents naked eye visible-agglutination assay for HIV) is a hemagglutination-based test for
American Society for Microbiology.
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8. Solid-phase enzyme-linked immunosorbent assay for detection of hepatitis A virus.
An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of hepatitis A virus in human fecal specimens. Investigations with 88 fecal specimens from 77 patients with suspected viral hepatitis and 8 of their household contacts showed that ELISA was as specific and sensitive as radioimmunoassay and almost as sensitive as immune electron micr
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9. Loop-Mediated Isothermal Amplification for Detection of African Trypanosomes
While PCR is a method of choice for the detection of African trypanosomes in both humans and animals, the expense of this method negates its use as a diagnostic method for the detection of endemic trypanosomiasis in African countries. The loop-mediated isothermal amplification (LAMP) reaction is a method that amplifies DNA with high specificity, efficiency,
American Society for Microbiology.
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10. Dot-immunogold filtration assay as a screening test for syphilis.
A dot-immunogold filtration assay (DIGFA) for the rapid detection of reaginic antibody in the serum of syphilitic patients was developed. The assay was simple, rapid, and reproducible. The test completion time was 2 min, and the assay required no equipment. The positive dot was very obvious, and the results could easily be determined with the naked eye. A to
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11. Comparison of a Nonradiometric Liquid-Medium Method (MB REDOX) with the BACTEC System for Growth and Identification of Mycobacteria in Clinical Specimens
Early identification of tuberculosis in the clinical setting is of great importance in order for specific therapy to be swiftly initiated. MB REDOX (Heipha Diagnostika), a growth-based medium without radioactive materials, was evaluated and was compared to the BACTEC system for detection of mycobacteria, including the Mycobacterium tuberculosis complex and a
American Society for Microbiology.
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12. A novel method to chemically immobilize antibody on nylon and its application to the rapid and differential detection of two Vibrio parahaemolyticus toxins in a modified enzyme-linked immunosorbent assay.
A new method of chemically immobilizing antibody on nylon was developed. The method consists of serial treatments with HCl, polyethylene imine, and maleic anhydride methylvinyl ether copolymer, which resulted in the stable immobilization of sufficient amounts of antibodies on nylon. This principle was used to differentially detect two immunologically related