Nitrogen Metabolism Evolution
Mostrando 1-12 de 16 artigos, teses e dissertações.
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1. Hipótese evolutiva sobre a assimilição de compostos nitrogenados por metazoários: a limitação α-aminoácidos / Evolutionary hypothesis on the nitrogenous compounds uptake by metazoan: the limitation to α-aminoacids
Metabolic pathway evolution models are molecular and computational based, and do not take account the physiological and ecological contexts in which organisms are inserted. Thus using the nitrogen metabolism as a platform, an evolutionary hypothesis on the α-amino acids utilization by metazoans was proposed. The objective of the present work is to trace
Publicado em: 2008
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2. Metabolite and light regulation of metabolism in plants: lessons from the study of a single biochemical pathway
We are using molecular, biochemical, and genetic approaches to study the structural and regulatory genes controlling the assimilation of inorganic nitrogen into the amino acids glutamine, glutamate, aspartate and asparagine. These amino acids serve as the principal nitrogen-transport amino acids in most crop and higher plants including Arabidopsis thaliana.
Brazilian Journal of Medical and Biological Research. Publicado em: 2001-05
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3. alpha-Hydroxylation pathway in the in vitro metabolism of carcinogenic nitrosamines: N-nitrosodimethylamine and N-nitroso-N-methylaniline.
Evolution of 15N2-labeled molecular nitrogen was used to gauge the extent of alpha-hydroxylation during rat liver homogenate metabolism of doubly 15N-labeled N-nitrosodimethylamine (DMN) and N-nitrosomethylaniline (NMA). These measurements were correlated with the extent of total metabolism as measured by the disappearance of the nitrosamines and by the form
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4. Metabolism of Radiolabeled β-Guaiacyl Ether-Linked Lignin Dimeric Compounds by Phanerochaete chrysosporium
Phanerochaete chrysosporium metabolized the radiolabeled lignin model compounds [γ-14C]guaiacylglycerol-β-guaiacyl ether and [4-methoxy-14C]veratrylglycerol-β-guaiacyl ether (VI) to 14CO2 in stationary and in shaking cultures. 14CO2 evolution was greater in stationary culture. 14CO2 evolution from [γ-14C]guaiacyl-glycerol-β-guaiacyl ether and [4-methoxy
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5. Pyruvate Metabolism, Carbon Dioxide Assimilation, and Nitrogen Fixation by an Achromobacter Species
Hamilton, I. R. (University of Wisconsin, Madison), R. H. Burris, P. W. Wilson, and C. H. Wang. Pyruvate metabolism and carbon dioxide assimilation by an Achromobacter species. J. Bacteriol. 89:647–653. 1965.—Carbon dioxide fixation by washed whole cells of Achromobacter N4-B has been observed during anaerobic pyruvate metabolism with both nitrogen- and
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6. Metabolism of Urea by Chlorella vulgaris1
Urea metabolism was studied with nitrogen-starved cells of Chlorella vulgaris Beijerinck var. viridis (Chodat), a green alga which apparently lacks urease. Incorporation of radioactivity from urea-14C into the alcohol-soluble fraction was virtually eliminated in cell suspensions flushed with 10% CO2 in air. This same result was obtained when expected accepto
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7. DL-7-azatryptophan and citrulline metabolism in the cyanobacterium Anabaena sp. strain 1F.
An alternative route for the primary assimilation of ammonia proceeds via glutamine synthetase-carbamyl phosphate synthetase and its inherent glutaminase activity in Anabaena sp. strain 1F, a marine filamentous, heterocystous cyanobacterium. Evidence for the presence of this possible alternative route to glutamate was provided by the use of amino acid analog
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8. Evolution of cytochrome oxidase, an enzyme older than atmospheric oxygen.
Cytochrome oxidase is a key enzyme in aerobic metabolism. All the recorded eubacterial (domain Bacteria) and archaebacterial (Archaea) sequences of subunits 1 and 2 of this protein complex have been used for a comprehensive evolutionary analysis. The phylogenetic trees reveal several processes of gene duplication. Some of these are ancient, having occurred i
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9. Stimulation of Ammonia and 2-Oxoglutarate-Dependent O2 Evolution in Isolated Chloroplasts by Dicarboxylates and the Role of the Chloroplast in Photorespiratory Nitrogen Recycling
Intact chloroplasts isolated from spinach (Spinacia oleracea L.) leaves showed a light-dependent O2 evolution (5.5 ± 0.75 micromoles per milligram chlorophyll per hour) when supplied with ammonia and 2-oxoglutarate. This (ammonia, 2-oxoglutarate)-dependent O2 evolution was stimulated 2- to 4-fold by the dicarboxylates, malate, succinate, fumarate, glutarate
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10. Evolution of the glutamine synthetase gene, one of the oldest existing and functioning genes.
We performed molecular phylogenetic analyses of glutamine synthetase (GS) genes in order to investigate their evolutionary history. The analyses were done on 30 DNA sequences of the GS gene which included both prokaryotes and eukaryotes. Two types of GS genes are known at present: the GSI gene found so far only in prokaryotes and the GSII gene found in both
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11. Interactions between the Nitrogen Signal Transduction Protein PII and N-Acetyl Glutamate Kinase in Organisms That Perform Oxygenic Photosynthesis
PII, one of the most conserved signal transduction proteins, is believed to be a key player in the coordination of nitrogen assimilation and carbon metabolism in bacteria, archaea, and plants. However, the identity of PII receptors remains elusive, particularly in photosynthetic organisms. Here we used yeast two-hybrid approaches to identify new PII receptor
American Society for Microbiology.
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12. Evidence for a conserved system for iron metabolism in the mitochondria of Saccharomyces cerevisiae
nifU of nitrogen-fixing bacteria is involved in the synthesis of the Fe–S cluster of nitrogenase. In a synthetic lethal screen with the mitochondrial heat shock protein (HSP)70, SSQ1, we identified a gene of Saccharomyces cerevisiae, NFU1, which encodes a protein with sequence identity to the C-terminal domain of NifU. Two other yeast genes were found to e
The National Academy of Sciences.