Nomarski Microscopy
Mostrando 1-12 de 18 artigos, teses e dissertações.
-
1. First report of Temnocephala rochensis (Platyhelminthes: Temnocephalida) from Pomacea canaliculata (Mollusca: Ampullariidae) outside Uruguay: description update based on specimens from the state of Rio Grande do Sul, Brazil
Temnocephala rochensis Ponce de Léon, 1979, was the second of four species of Temnocephala Blanchard, 1849 to be described as ectosymbiont of ampullariid apple snails, Pomacea canaliculata (Lamarck, 1822). There have been no records of this Uruguayan species after its initial description. As part of the present study, 111 specimens of P. canaliculata were c
Zoologia (Curitiba). Publicado em: 2010-10
-
2. Proposta de método para caracterização de propriedades termomecânicas de filmes finos utilizando dispositivos MEMS. / Proposition of thin films thermomechanical characterization using MEMS devices.
An important factor to develop microsystems is knowledge of materials properties and failure mechanisms. This research studies the thermal actuated microbeam mechanical behavior and propose a method in order to characterize thermomechanical properties of thin films. Silicon Oxynitride microbeams are fabricated and Nomarski microscopy was applied to observe s
Publicado em: 2002
-
3. Interference Contrast and Phase Contrast Microscopy of Sporulation and Germination of Bacillus megaterium
The techniques of Nomarski interference contrast microscopy and phase-contrast microscopy were compared for their utility in monitoring sporulation and germination in Bacillus megaterium. The Nomarski technique permitted rapid and easy delineation of septation and engulfment during sporulation, whereas with phase contrast microscopy these stages were not det
-
4. Visualization of bacterial flagella by video-enhanced light microscopy.
We have imaged individual flagellar filaments of Escherichia coli, a motile Streptococcus sp., and Rhizobium meliloti by video-enhanced differential interference-contrast microscopy (Nomarski DIC) and computer-based image processing. This approach has advantages over existing methods in that filaments on living cells can be seen over their entire lengths.
-
5. Sputum screening by Nomarski interference contrast microscopy.
Gram-stained smears of specimens submitted for sputum cultures were compared with direct wet mounts examined by Nomarski interference contrast microscopy (NIM) for enumeration of squamous epithelial cells (EPC) and leukocytes (WBC). The results obtained by the two methods were comparable, but specimens were more rapidly screened and cell types were more read
-
6. Techniques for examining Pneumocystis carinii in fresh specimens.
Pneumocystis carinii was examined in fresh preparations of infected rat lung homogenates and tissue culture supernatants by a variety of light microscope techniques, vital dyes, and histologic stains. Phase-contrast microscopy, Nomarski interference-contrast microscopy, and bright-field microscopy with oblique illumination provided excellent views of P. cari
-
7. Interference-Contrast and Phase-Contrast Microscopy of Sporulation in Clostridium thermosaccharolyticum Grown Under Strict Anaerobiosis
Cells of Clostridium thermosaccharolyticum grown under strict anaerobiosis (modified Hungate technique) were examined during growth and sporulation by employing Nomarski interference-contrast and Zernike phase-contrast optics to delineate the sequence of morphological changes leading to the formation of free, mature spores. A 0.5% l-arabinose, liquid, comple
-
8. Morphology and ultrastructure of Crenothrix polyspora Cohn.
Naturally grown cell material of Crenothrix polyspora from the well of a waterworks was studied by means of phase-contrast and Nomarski interference microscopy as well as by transmission electron microscopy. The material consisted of clusters of sheathed filaments up to 2 cm long. Propagation forms observed were nonmotile, spherical cells that arose by simpl
-
9. Effect of human leukocyte interferon on invasiveness of Salmonella species in HEp-2 cell cultures.
The effect of human leukocyte interferon on the invasiveness of Salmonella and Shigella species in HEp-2 cell cultures was examined. The intracellular and extracellular bacteria were identified by a combination of Nomarski differential interference contrast microscopy and UV incident light microscopy applied on the same microscope. Pretreatment of HEp-2 cell
-
10. Measurement of live bacteria by Nomarski interference microscopy and stereologic methods as tested with macroscopic rod-shaped models.
A new method is proposed to measure bacterial cells under growth conditions. Bacterial cells, suspended in their growth medium, were attached to a cover slip with poly-L-lysine. The cover slip was inverted and placed on a glass microscope slide. To prevent dehydration of the medium, the edges of the cover slip were sealed to the microscope slide with clear f
-
11. An in vitro method for detecting infectious Cryptosporidium oocysts with cell culture.
Current assay methods to detect Cryptosporidium oocysts in water are generally not able to evaluate viability or infectivity. A method was developed for low-level detection of infective oocysts by using HCT-8 cells in culture as hosts to C. parvum reproductive stages. The infective foci were detected by labeling intracellular developmental stages of the para
-
12. The Ncl-1 Gene and Genetic Mosaics of Caenorhabditis Elegans
A ncl-1 mutation results in enlarged nucleoli, which can be detected in nearly all cells of living animals by Nomarski microscopy. Spontaneous mitotic loss of a ncl-1(+)-containing free duplication in an otherwise homozygous ncl-1 mutant animal results in mosaicism for ncl-1 expression, and the patterns of mosaicism lead us to conclude that ncl-1 acts cell a