Nuclease Protection Assays Methods
Mostrando 1-4 de 4 artigos, teses e dissertações.
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1. "Determinação do perfil de expressão dos RNAs mensageiros da família das Smads e dos componentes do complexo AP-1 em carcinoma de célula escamosa de cabeça e pescoço" / Smads and AP-1 messenger RNA expression pattern in Head and Neck Squamous Cell Carcinoma
Smad and AP1 messenger RNA expression may underlie disruptions affecting TGFb signaling in head and neck squamous cell carcinoma (HNSCC). Analysis of Smads1-8 mRNA expression by RPA has shown Smad expression is globally increased in tumor as compared to adjacent normal tissue. Kaplan Meier survival curves and multivariate analysis revealed that Smad6 positiv
Publicado em: 2005
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2. Transcriptional Organization of the Erythromycin Biosynthetic Gene Cluster of Saccharopolyspora erythraea
The transcriptional organization of the erythromycin biosynthetic gene (ery) cluster of Saccharopolyspora erythraea has been examined by a variety of methods, including S1 nuclease protection assays, Northern blotting, Western blotting, and bioconversion analysis of erythromycin intermediates. The analysis was facilitated by the construction of novel mutants
American Society for Microbiology.
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3. Methods to investigate the expression of lignin peroxidase genes by the white rot fungus Phanerochaete chrysosporium.
Two methods allowing the analysis of expression of specific lignin peroxidase (LPO) genes from white rot fungi are presented. In the first method, degenerate oligonucleotide primers derived from amino acid sequence motifs held in common among all members of the LPO gene family are used to prime the polymerase chain reaction (PCR) amplification of LPO-related
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4. Genomic RNAs of influenza viruses are held in a circular conformation in virions and in infected cells by a terminal panhandle.
The viral RNA segments in influenza virions were shown to be circular in conformation by using psoralen crosslinking methods. Electron microscopy of purified RNA following treatment of virus with the psoralen reagent 4'-aminomethyltrioxsalen (AMT) revealed circles with lengths corresponding to the individual segments. RNA blot analysis using polyacrylamide g