Nucleic Acid Hybridization
Mostrando 1-12 de 462 artigos, teses e dissertações.
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1. Specific Disulfide Bond Formation Directed by Peptide Nucleic Acids
In this study, we successfully demonstrated a strategy for specific disulfide bond formation by hybridization of two complementary peptide nucleic acid strands. Peptides were linked to the pair of complementary peptide nucleic acids by native chemical ligation, respectively, and base pairing drove the two peptides into close proximity for interchain disulfid
J. Braz. Chem. Soc.. Publicado em: 2020-05
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2. BIOSSENSOR ELETROQUÍMICO PARA VIROSES A PARTIR DE TÉCNICAS MOLECULARES / BIOSENSOR VIROSES FOR ELECTROCHEMICAL TECHNIQUES FROM MOLECULAR
Os vírus são micro-organismos que podem causar doenças. O vírus da dengue (DENV) apresenta 4 sorotipos relacionados que se caracteriza por um estado febril, ou pode progredir para a dengue hemorrágica caracterizada por manifestações como a síndrome hemorrágica de choque na dengue. Outro vírus causador de doença em humanos, o papilomavírus humano
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 12/08/2011
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3. Abortamento humano : detecção molecular de AAV e de HPV em decídua e vilosidade coriônica
Pregnancy failure is a common event often of unknown cause. Some viruses are suggested as cause of abortion and, among them, adeno-associated virus (AAV), that has never been implicated as a cause of disease. AAV (AAV1-11 types), belonging to Parvoviridae family, requires helper virus function for replication, as from human papillomavirus (HPV), that belongs
Publicado em: 2007
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4. Comparação entre teste bana e checkerboard dna-dna hybridization no diagnóstico periodontal inicial e no monitoramento terapêutico
The aim of this study was tom compare the results of the BANA with the presence of the red complex microorganisms established by the Checkerboard DNA-DNA hybridization in the initial diagnosis and in the therapeutic maintenance of subjects with periodontitis. 54 subjects with chronic periodontitis with at least 15 teeth were selected. Subgingival plaque samp
Publicado em: 2005
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5. Gene expression in IFN-gamma-activated murine macrophages
Macrophages are critical for natural immunity and play a central role in specific acquired immunity. The IFN-gamma activation of macrophages derived from A/J or BALB/c mice yielded two different patterns of antiviral state in murine hepatitis virus 3 infection, which were related to a down-regulation of the main virus receptor. Using cDNA hybridization to ev
Brazilian Journal of Medical and Biological Research. Publicado em: 2004-12
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6. Dengue: a review of the laboratory tests a clinician must know to achieve a correct diagnosis
Dengue is the most important disease caused by an arbovirus (1, 2, 3 and 4 serotypes) worldwide, especially in the tropical and sub-tropical regions. Its clinical manifestations range from asymptomatic infections to a severe disease characterized by hemorrhage and shock. The incidence of dengue virus activity in the Americas has substantially increased from
Brazilian Journal of Infectious Diseases. Publicado em: 2004-12
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7. Detection of group A beta-hemolytic Streptococcus employing three different detection methods: culture, rapid antigen detecting test, and molecular assay
In order to study the prevalence of Group A beta-hemolytic Streptococcus (GABHS) pharyngotonsillitis in our pediatric population and to compare different sampling methods of GABHS detection, oropharyngeal swabs from 50 children with acute pharyngotonsillitis, between 1 and 12 years old, were used simultaneously for culture, molecular assay and rapid GABHS an
Brazilian Journal of Infectious Diseases. Publicado em: 2003-10
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8. IS1245 restriction fragment length polymorphism typing of Mycobacterium avium from patients admitted to a reference hospital in Campinas, Brazil
Mycobacterium avium is an important pathogen among immunodeficient patients, especially patients with AIDS. The natural history of this disease is unclear. Several environmental sources have been implicated as the origin of this infection. Polyclonal infection with this species is observed, challenging the understanding of its pathogenesis and treatment. In
Brazilian Journal of Medical and Biological Research. Publicado em: 2003-10
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9. Nucleic acid scanning-by-hybridization of enterohemorrhagic Escherichia coli isolates using oligodeoxynucleotide arrays.
Nucleic acid scanning by hybridization (NASBH) is a non-electrophoretic typing strategy that uses gridded oligonucleotides to reproducibly characterize arbitrarily amplified nucleic acid sequences. Membrane-bound arrays of terminally-degenerate oligonucleotides were hybridized to DNA amplification fingerprinting (DAF) products from enterohemorrhagic Escheric
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10. Differentiation of respiratory syncytial virus subgroups with cDNA probes in a nucleic acid hybridization assay.
A new approach to respiratory syncytial (RS) virus subgroup determination was developed by using a simple nucleic acid filter hybridization technique. By this method, virus-infected cells are bound and fixed in a single step, and the viral RNA in the fixed-cell preparation is characterized directly by its ability to hybridize to cDNA probes specific for eith
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11. A homogeneous nucleic acid hybridization assay based on strand displacement.
A homogeneous nucleic acid hybridization assay which is conducted in solution and requires no separation steps is described. The assay is based on the concept of strand displacement. In the strand displacement assay, an RNA "signal strand" is hybridized within a larger DNA strand termed the "probe strand", which is, in turn, complementary to the target nucle
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12. Auxin and Ribonucleic Acid Synthesis in Pea Stem Tissue as Studied by Deoxyribonucleic Acid-Ribonucleic Acid Hybridization 1
The ability of auxin to alter gene transcription in pea (Pisum sativum L.) stem tissues has been investigated by means of DNA/RNA hybridization-competition techniques. In order to obtain reproducible hybridization with total nucleic acid preparations from plants it was found necessary to remove interfering substances, probably polysaccharides; this was accom