Octyl Sepharose
Mostrando 1-12 de 44 artigos, teses e dissertações.
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1. IDENTIFICAÇÃO DE PROTEÍNAS ANTIMICROBIANAS DE FLORES DE ALECRIM-PIMENTA (Lippia sidoides): UMA NOVA ESTRATÉGIA NO COMBATE A PATÓGENOS
Um dos principais problemas mundiais na agricultura está diretamente relacionado às enormes perdas na produção causada por fungos fitopatogenicos, onde sua infecção nas culturas se dá desde o plantio até a pós- colheita. O fungo Botrytis cinerea, causador do mofo cinzento em mais de 200 espécies de plantas é um grande problema no agronegócio em t
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 12/03/2009
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2. Estudo da multiplicidade de formas de ß-glicosidases de Aspergillus versicolor / Study of ß-glycosidases from Aspergillus versicolor
Este trabalho procurou avaliar algumas isozimas envolvidas com o processo de degradação de polissacarídeos da parede celular vegetal, encontradas em Aspergillus versicolor, fungo pertencente a um gênero onde a multiplicidade de componentes enzimáticos com a mesma atividade oscila de uma a três. Duas ß-xilosidases foram purificadas por meio de DEAE-cel
Publicado em: 2008
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3. Isolamento de bactérias resistentes a cromo hexavalente e purificação parcial da enzima redutora de cromo do Bacillus sp. ES29
A redução do Cr(VI) para Cr(III) diminui a toxidade deste metal no ambiente uma vez que, o Cr(III) é insolúvel às membranas biológicas. Assim a redução microbiana do Cr(VI) é uma alternativa para reduzir os impactos ambientais causados por este metal, utilizado em diversos processos industriais. O objetivo deste trabalho foi selecionar microrganismo
Publicado em: 2007
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4. Papel dos (glico)esfingolipídeos e proteofosfoglicanos de Leishmania (Leishmania) amazonensis na infecção de macrófagos. Caracterização de novos antígenos e isolamento de microdomínios de membranas resistentes a detergente não-iônico. / Role of (glyco)sphingolipids and proteophosphoglycans of Leishmania (Leishmania) amazonensis on macrophage infection. Characterization of novel antigens and isolation of non-ionic detergent-resistant membrane microdomains.
As leishmanioses constituem um importante problema de saúde pública no Brasil, em razão da incidência da doença associada à alta taxa de morbidade. Devido ao fato da Leishmania ser um parasita intracelular no hospedeiro vertebrado, estudos de moléculas do parasita, que atuam na interação do parasita com a célula do hospedeiro vertebrado, são de al
Publicado em: 2006
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5. Distribution of small proteoglycans and glycosaminoglycans in humerus-related articular cartilage of chickens
The expression of components present in the cartilaginous extracellular matrix is related to development, gender, and genotype, as well as to the biomechanical properties of each type of cartilage. In the present study, we analyzed small proteoglycans and glycosaminoglycans present in different cartilages of the chicken wing after extraction with guanidine h
Brazilian Journal of Medical and Biological Research. Publicado em: 2005-03
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6. Characterization of Virus Adsorption by Using DEAE-Sepharose and Octyl-Sepharose†
Viruses were characterized by their adsorption to DEAE-Sepharose or by their elution from octyl-Sepharose by using buffered solutions of sodium chloride with different ionic strengths. Viruses whose adsorption to DEAE-Sepharose was reduced most rapidly by an increase in the sodium chloride concentration were considered to have the weakest electrostatic inter
American Society for Microbiology.
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7. Properties of binding of Escherichia coli endotoxin to various matrices.
Binding of Escherichia coli O127:B8 endotoxin to a variety of resins and column materials was investigated by measuring the beta-hydroxy myristic acid content (a major component of the lipid A moiety) of endotoxin after hydrolysis by selected ion-monitoring gas chromatography-mass spectrometry. More than 80% of the endotoxin was bound to hydroxylapatite, pol
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8. Inhibition of the interaction of Streptococcus sanguis with hexadecane droplets by 55- and 60-kilodalton hydrophobic proteins of human saliva.
The effect of salivary secretions on the hydrophobicity of Streptococcus sanguis was investigated. Pretreatment of the bacteria with paraffin-stimulated whole saliva resulted in a 79% inhibition of adhesion to hexadecane droplets. Column chromatography on Sepharose 4B and sodium dodecyl sulfate gel electrophoretic analysis indicated that the inhibitory activ
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9. Extracellular lipase of Pseudomonas sp. strain ATCC 21808: purification, characterization, crystallization, and preliminary X-ray diffraction data.
A procedure for the purification of a very hydrophobic lipase from Pseudomonas sp. strain ATCC 21808 was elaborated by avoiding the use of long-chain detergents in view of subsequent crystallization of the enzyme. The purification procedure included chromatography on Q-Sepharose in the presence of n-octyl-beta-D-glucopyranoside, Ca2+ precipitation of fatty a
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10. Purification of photochemically active halorhodopsin.
We have developed a procedure for the purification of halorhodopsin in a photochemically active state. Solubilization of membranes from a bacteriorhodopsin-negative Halobacterium strain with octyl glucoside was followed by chromatography on hydroxylapatite and octyl-Sepharose gels. All steps were carried out in high-ionic-strength solutions. The procedure re
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11. Bioluminescence assay for estimating the hydrophobic properties of bacteria as revealed by hydrophobic interaction chromatography.
The luciferin-luciferase bioluminescence method was used to estimate the number of bacteria retained in neutral and amphiphilic gels and those in the eluate to determine the hydrophobic surface properties of bacteria by using hydrophobic interaction chromatography. Good correlations were found between viable counts and ATP content for Escherichia coli, Pseud
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12. Physicochemical surface properties of Escherichia coli strains isolated from different types of urinary tract infections.
Escherichia coli strains isolated from three groups of patients with urinary tract infections, such as acute pyelonephritis, acute cystitis, and asymptomatic bacteriuria, were analyzed with respect to their physicochemical surface properties by means of polymer two-phase partitioning in dextran-polyethylene glycol systems and hydrophobic interaction chromato