Oligonucleotide Array Sequence Analysis Methods
Mostrando 1-7 de 7 artigos, teses e dissertações.
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1. Expressão gênica diferencial das células estromais obtidas de medula óssea na presença ou ausência de célula tumoral oculta em pacientes com câncer de mama / Differential gene expression of bone marrow stromal cells from breast cancer patients in the presence or abscence of occult tumor cells
Stromal cells may influence tumor development in primary and secundary sites, however, molecular characteristics of bone marrow stromal cells from breast cancer patients are almost unknown. Our aim was to evaluate the differential gene expression of bone marrow stromal cells from breast cancer patients in the presence or abscence of occult tumor cells. Bone
Publicado em: 2006
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2. Prokaryotic RNA preparation methods useful for high density array analysis: comparison of two approaches
High density oligonucleotide arrays have been used extensively for expression studies of eukaryotic organisms. We have designed a prokaryotic high density oligonucleotide array using the complete Escherichia coli genome sequence to monitor expression levels of all genes and intergenic regions in the genome. Because previously described methods for preparing
Oxford University Press.
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3. Parallel gene analysis with allele-specific padlock probes and tag microarrays
Parallel, highly specific analysis methods are required to take advantage of the extensive information about DNA sequence variation and of expressed sequences. We present a scalable laboratory technique suitable to analyze numerous target sequences in multiplexed assays. Sets of padlock probes were applied to analyze single nucleotide variation directly in t
Oxford University Press.
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4. Rapid methods for population-scale analysis for gene polymorphisms: the ACE gene as an example.
OBJECTIVE--To obtain rapid, high throughput genotyping of the angiotensin converting enzyme (ACE) gene intron 16 insertion/deletion polymorphism. METHODS--DNA was obtained from whole blood samples by a simple liquid phase methanol extraction procedure. The ACE gene was amplified by the polymerase chain reaction (PCR) using two oligonucleotide primers (ACE1 a
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5. Single-well genotyping of diallelic sequence variations by a two-color ELISA-based oligonucleotide ligation assay.
Single nucleotide substitutions and unique insertions/deletions are the most common form of DNA sequence variation and disease-causing mutation in the human genome. Because of the biological and medical importance of these variations, a wide array of methods have been developed for their typing. We have applied an approach that combines the amplification of
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6. Characterization of synthetic DNA bar codes in Saccharomyces cerevisiae gene-deletion strains
Incorporation of strain-specific synthetic DNA tags into yeast Saccharomyces cerevisiae gene-deletion strains has enabled identification of gene functions by massively parallel growth rate analysis. However, it is important to confirm the sequences of these tags, because mutations introduced during construction could lead to significant errors in hybridizati
National Academy of Sciences.
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7. Detection of Deleted Genomic DNA Using a Semiautomated Computational Analysis of GeneChip Data
Genomic diversity within and between populations is caused by single nucleotide mutations, changes in repetitive DNA systems, recombination mechanisms, and insertion and deletion events. The contribution of these sources to diversity, whether purely genetic or of phenotypic consequence, can only be investigated if we have the means to quantitate and characte
Cold Spring Harbor Laboratory Press.