Oocyte Activation
Mostrando 1-12 de 186 artigos, teses e dissertações.
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1. Stress, strain, and pregnancy outcome in postpartum cows
Abstract Stress affects the productivity and fertility of cattle. Stress causes strain and individual animals experience different amounts of strain in response to the same amount of stress. The amount of strain determines the impact of stress on fertility. Typical stresses experienced by cattle include environmental, disease, production, nutritional, and ps
Anim. Reprod.. Publicado em: 28/11/2019
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2. Powdered coconut water (ACP 406®) as an alternative base culture medium for in vitro culture of goat preantral follicles enclosed in ovarian tissue
Abstract This study evaluated a powdered coconut water solution (ACP 406®) as a base culture medium on the in vitro survival and development of in situ goat preantral follicles. The ovarian fragments were either immediately fixed in Carnoy solution (non-cultured control) or individually cultured for 2 or 6 days. The following culture media (all containing 1
Anim. Reprod.. Publicado em: 25/11/2019
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3. Anethole reduces oxidative stress and improves in vitro survival and activation of primordial follicles
Primordial follicles, the main source of oocytes in the ovary, are essential for the maintenance of fertility throughout the reproductive lifespan. To the best of our knowledge, there are no reports describing the effect of anethole on this important ovarian follicle population. The aim of the study was to investigate the effect of different anethole concent
Braz J Med Biol Res. Publicado em: 28/05/2018
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4. Effects of the spermatozoa: oocyte ratio, water volume and water temperature on artificial fertilization and sperm activation of cascudo-preto
The objective of this study was to evaluate the effects of water volume and water temperature on the sperm motility duration and the number of spermatozoa, and the water volume on the fertilization rates of oocytes of Rhinelepis aspera. Experiments were carried out to evaluate the effect of semen dilutions (1.74×10-5, 1.74×10-4, 1.74×10-3, 1.74×10-2, 1.7
R. Bras. Zootec.. Publicado em: 2013-01
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5. Novel insights into the genetic and epigenetic paternal contribution to the human embryo
The integrity of the sperm genome and epigenome are critical for normal embryonic development. The advent of assisted reproductive technology has led to an increased understanding of the role of sperm in fertilization and embryogenesis. During fertilization, the sperm transmits not only nuclear DNA to the oocyte but also activation factor, centrosomes, and a
Clinics. Publicado em: 2013
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6. EFEITO DA ASSOCIAÇÃO DO FATOR DE CRESCIMENTO E DIFERENCIAÇÃO 9 (GDF-9) E DO HORMÔNIO FOLÍCULO ESTIMULANTE (FSH) SOBRE O DESENVOLVIMENTO IN VITRO DE FOLÍCULOS PRÉ-ANTRAIS CAPRINOS EM CULTIVO IN SITU DE LONGA DURAÇÃO / EFFECT OF ASSOCIATION AND GROWTH FACTOR DIFFERENTIATION 9 (GDF-9) and hormone FOLLICLE STIMULATING (FSH) DEVELOPMENT OF IN VITRO PREANTRAL FOLLICLES GOATS IN FARMING IN SITU LONG
The aim of this study was to evaluate the effects of growth differentiation factor 9 (GDF-9) and follicle-stimulating hormone (FSH) on the in vitro survival, growth and steroidogenesis of caprine preantral follicles cultured for 16 days. Ovarian fragments were cultured in αMEM+ in the presence or absence of GDF-9 (200 ng/mL) and/or FSH (50 ng/mL) added
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 02/07/2012
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7. Parthenogenetic activation of bovine oocytes using single and combined strontium, ionomycin and 6-dimethylaminopurine treatments.
In vitro-matured (IVM) bovine oocytes were activated with single and combined treatments of strontium (S), ionomycin (I) and 6-DMAP (D). Using oocytes IVM for 26 h, we observed that activation altered cell cycle kinetics (faster progression, MIII arrest, or direct transition from MII to pronuclear stage) when compared to in vitro fertilization. The effect of
Zygote. Publicado em: 2011
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8. Oocyte activation and preimplantation development of bovine embryos obtained by specific inhibition of cyclin-dependent kinases.
Realizaram-se dois experimentos para avaliar a eficiência da bohemina e roscovitina associadas à ionomicina para ativação partenogenética e desenvolvimento embrionário inicial de bovinos. No primeiro, foram testadas diferentes concentrações (0, 50, 75 ou 100µM) e diferentes tempos de exposição (2, 4 ou 6 horas) à bohemina ou à roscovitina na ati
Arquivo Brasileiro de Medicina Veterinaria e Zootecnia.. Publicado em: 2011
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9. Proteína quinase C (PKC) e proteína quinase dependente de cálcio/calmodulina (CaMK II) na ativação de oócitos bovinos / Protein kinase C (PKC) and Calcium/calmodulin-dependent protein kinase II (CaMKII) in bovine oocyte activation
A fecundação resulta no aumento intracelular de cálcio que é necessário para a transição do oócito até o estádio de zigoto. Os eventos que ocorrem durante esta transição são caracterizados como ativação, sendo estes dependentes de cálcio. Entretanto, os eventos bioquímicos que ocorrem durante a ativação ainda não estão completamente eluc
Publicado em: 2010
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10. Effects of the conditioned medium of mesenchymal stem cells on mouse oocyte activation and development
Mesenchymal stem cells (MSCs) have been reported to secrete a variety of cytokines and growth factors acting as trophic suppliers, but little is known regarding the effects of conditioned medium (CM) of MSCs isolated from femurs and tibias of mouse on the artificial activation of mouse oocytes and on the developmental competence of the parthenotes. In the cu
Brazilian Journal of Medical and Biological Research. Publicado em: 2009-06
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11. Ativação de genes apoptóticos no bloqueio do desenvolvimento em embriões bovinos / Activation of apoptotic genes at developmental block in bovine embryo development
Maternal-zygotic transition is a complex phenomenon characterized by the initiation of transcription in the embryo and the transition of maternal mRNA with embryonic mRNA. It is believed that the mRNAs and proteins synthethized by the oocyte during its growth and final maturation allow the zygote to develop during the early stages of embryo development up to
Publicado em: 2009
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12. Efeito da pré-maturação sobre o desenvolvimento embrionário de oócitos submetidos à ativação partenogenética e transferência de núcleo / Effect of pre-maturation on embryo development of oocytes submitted to parthenogenetic activation and nuclear transfer
Embryo production rates obtained from both IVF (30-40%) and NT (23%) are still below the expected values. Therefore, oocyte pre-maturation using cell cycle inhibitors is one of the alternatives which has been studied to increase the competence of oocytes used for IVP, as an attempt to optimize the success rates of these biotechniques. Neurotrophins are known
Publicado em: 2009