Ooplasmic
Mostrando 1-5 de 5 artigos, teses e dissertações.
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1. Pronounced segregation of donor mitochondria introduced by bovine Ooplasmic tranfer to the female germ-line.
Ooplasmic transfer (01) has been used in basic mouse research for studying lhe segregation of mtDNA, as well as in human assisted reproduction for improving embryo development in cases of persistent developmental failure. Using cattle as a large-animal model, we demonstrate that lhe moderate amount of mitochondria introduced by OT is transmitted to lhe offsp
Biology of Reproduction. Publicado em: 2011
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2. Karyoplast exchange between strontium- and 6-DMAP-parthenogenetically activated zygotes of cattle.
Ooplasmic factors drive nuclear organization after fertilization and are also important for re-programming in nuclear transfer procedures, in which artificial activation is essential for reconstructed embryos to progress in development. The present research evaluated the effect of pronuclear transfer (PT) between zygotes parthenogenetically activated with io
Animal Reproduction Science. Publicado em: 2011
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3. Artifacts caused by cell microinjection.
The effects of microinjection on Rana pipiens oocytes were determined using cryomicrodissection to measure Na, K, water, and injected radiolabeled sucrose (in gelatin) in the nucleus, animal, and vegetal ooplasm and injected bolus (reference phase, RP). The results point to potential problems in the interpretation of microinjection experiments. When oocytes
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4. In vivo stabilization of the Dnmt1 (cytosine-5)- methyltransferase protein
The Dnmt1o form of the Dnmt1 (cytosine-5)-methyltransferase enzyme is synthesized and stored in the cytoplasm of the oocyte and is used after fertilization to maintain methylation patterns on imprinted genes. After implantation of the blastocyst, Dnmt1o is replaced by the Dnmt1 form, which has an additional 118 aa at its amino terminus. To investigate functi
National Academy of Sciences.
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5. Determination of excitability types in blastomeres of the cleavage-arrested but differentiated embryos of an ascidian.
Cleavage of the embryo of Halocynthia roretzi was arrested with cytochalasin B at 1- to 32-cell stages and the embryo was cultured in sea water containing cytochalsin B until a developmental time equivalent to the hatching of the control larva. Membrane properties of the blastomeres were studied with constant-current and voltage-clamp techniques. Four types