Opaque Mutant
Mostrando 1-12 de 41 artigos, teses e dissertações.
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1. Storage proteins characterization of high-lysine maize endosperm mutants / Caracterização de proteínas de reserva de mutantes de endosperma de milho de alta lisina
A semente de milho representa uma importante fonte de proteínas para alimentação humana e de animais monogástricos. Porém, como membros da família dos cereais não apresentam proteínas com um balanço nutricional adequado, devido principalmente ao baixo conteúdo de lisina. As proteínas de reserva da semente de milho são classificadas como fração
Publicado em: 2006
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2. Gama -coixina : isolamento do clone genomico e caracterização da região promotora
Prolamins, the major cereal storage proteins, are encoded by genes coordinately regulated in a tissue- and developmental stage-specific manner during seed development. There are several prolamin genes encoding proteins of discrete molecular sizes, which are divided in classes according to similarities in their primary structures and solubility. The expressio
Publicado em: 1996
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3. Mutantes da via metabolica do acido aspartico em milho, obtidos atraves de cultura de tecidos
The essential amino acids lysine, threonine and methionine are derived from the aspartate family of amino acids in pIants. Since the cereaIs are devoid in at least one of these amino acids, the aspartate pathway has received great attention in the last recent years. Biochemical, moIecular and genetic studies has lead to a deep understand of the regulation of
Publicado em: 1988
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4. Estudo sobre a enzima -cetoglutarato redutase no milho
Maise is considered a poor source of proteins for mammals during growth, in view of its relatively low levels of some essential amino acids in the endosperm, such as lysine. Thus, the discovery that the opaque-2 mutant gene nutritional value of maize has stimulated research improves the into the mechanism underlying this alteration, together with the selecti
Publicado em: 1988
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5. Estudo de efeitos dos genes opaque-2, shruken e shrunken-2 durante a germinação do milho
Abstract: Three isogenic inbreads of maize were used to study the effect of the opaque-2 (o2)~ shrunken (sh) and shrunken-2 (sh2) genes during germination. One inbread had two types of endosperm; one homozygote for the 02 gene and the other for the 02 gene. A second line had also two different endosperms: one homozygote for the sh allele and the other for th
Publicado em: 1983
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6. Comportamento de mutantes de endosperma de milho (zea mays L.) em relação ao fuzarium moniliforme Sheld
A comparative study involving 11 different endosperm mutants of maize and their correspondent normal counterparts was carried out to study the effect of endosperm mutant genes on pathogenicity of F. moniliforme. The following mutants were studied: Shrunken (sh), shrunken-2 (sh2), floury (fl), brittle (bt) brittle-2 (bt2), waxy (wx), dull (du), sugary (su), s
Publicado em: 1982
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7. O potencial osmotico em mutantes de endosperma de milho e sua interação com caracteristicas fisicas e com a atividade da RNase da semente
Four near isogenic inbreds of maize sinlge mutants homozygous for the opaque-2, shrunken, shrunken-2 and sugary genes, and their normal counterparts, as well as the single cross HS 7777, were used to study the reIationship among osmotic potential, dry weight, water content and RNase activity in the endosperm. The plants were grown on adjoining plots ate the
Publicado em: 1982
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8. EFG1 Null Mutants of Candida albicans Switch but Cannot Express the Complete Phenotype of White-Phase Budding Cells
The Candida albicans gene EFG1 encodes a putative trans-acting factor. In strain WO-1, which undergoes the white-opaque transition, EFG1 is transcribed as a 3.2-kb mRNA in white-phase cells and a less-abundant 2.2-kb mRNA in opaque-phase cells. cDNA sequencing and 5′ rapid amplification of cDNA ends analysis demonstrate that the major difference in molecul
American Society for Microbiology.
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9. Dominant Negative Mutants of Opaque2 Suppress Transactivation of a 22-kD Zein Promoter by Opaque2 in Maize Endosperm Cells.
In maize endosperm, genes encoding the 22-kD zein class of storage proteins are regulated by the OPAQUE2 locus. The Opaque2 (O2) protein shares homology with the basic domain/leucine zipper class of transcriptional activators. Using microprojectile bombardment, we have shown that O2 is capable of transactivating a 22-kD zein promoter in maize endosperm suspe
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10. opaque-2 modifiers increase gamma-zein synthesis and alter its spatial distribution in maize endosperm.
Through the action of opaque-2 modifier genes, the soft, floury endosperm of opaque-2 mutants is converted to a vitreous phenotype. This change in endosperm texture is associated with a twofold to threefold increase in gamma-zein content. To investigate the effect of opaque-2 modifiers on the expression of gamma-zein genes, we analyzed the synthesis and dist
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11. Influence of Opaque-2 and Floury-2 Genes on Formation of Proteins in Particulates of Corn Endosperm
Protein-rich subcellular particulates were isolated by zonal centrifugation from homogenates of endosperms of normal, opaque-2, and floury-2 mutant corn (Zea maize) kernels at different stages of development. In early stages the high lysine mutants vary from normal corn by greater production of a glutelin protein not associated with the matrix. This protein
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12. Roles of TUP1 in Switching, Phase Maintenance, and Phase-Specific Gene Expression in Candida albicans
Candida albicans strain WO-1 switches spontaneously and reversibly between a “white” and “opaque” phenotype that affects colony morphology, cellular phenotype, and expression of a number of phase-specific genes and virulence traits. To assess the role of the transcription regulator Tup1p in this phenotypic transition, both TUP1 alleles were deleted i
American Society for Microbiology.