Optimal Clones
Mostrando 1-12 de 92 artigos, teses e dissertações.
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1. Exigência nutricional e produtividade em minijardim clonal de Toona ciliata var. australis
Resumo O estado nutricional da planta-matriz é imprescindível para a manutenção do seu vigor vegetativo, fundamental para a produção de miniestacas. Portanto, o fornecimento de nutrientes em quantidades ideais durante o manejo das minicepas influencia o sucesso da propagação. Objetivou-se com este estudo avaliar e comparar a produtividade do minijard
Ciênc. Florest.. Publicado em: 02/12/2019
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2. Isolation and characterization of a novel endo-β-1,4-glucanase from a metagenomic library of the black-goat rumen
ABSTRACT The various types of lignocellulosic biomass found in plants comprise the most abundant renewable bioresources on Earth. In this study, the ruminal microbial ecosystem of black goats was explored because of their strong ability to digest lignocellulosic forage. A metagenomic fosmid library containing 115,200 clones was prepared from the black-goat r
Braz. J. Microbiol.. Publicado em: 2017-12
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3. Mass spore production and inoculation of Calonectria pteridis on Eucalyptus spp. under different environmental conditions
Calonectria pteridis is one of the most important causal agents of Calonectria leaf blight (CLB) of Eucalyptus spp. in Brazil, which is a limiting factor for growth of Eucalyptus plantations. In this study we developed standard procedures for in vitro mass spore production and inoculation of C. pteridis for screening plant species and clones for resistance t
Trop. plant pathol.. Publicado em: 2013-10
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4. Métodos para resgate, conservação e multiplação em larga escala de matrizes de Eucalyptus benthamii Maiden &Cambage / Methods for rescue, conservation and multiplication of matrices Eucalyptus benthamii Maiden &Cambege
The E. benthamii presents a great aptitude for cultivation in Brazil, especially in cold climates and with frequent frosts, this is due to your west of Sydney origin in Australia, where temperatures are very low, with frosts. Taking into account their optimal silvicultural performance, selected genotypes will certainly represent an excellent alternative for
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 28/06/2012
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5. Abordagem algébrica para seleção de clones ótimos em projetos genomas e metagenomas / Algebraic approach to optimal clone selection in genomics and metagenomics projects.
Devido à grande diversidade de microrganismos desconhecidos no meio ambiente, 99% deles não podem ser cultivados nos meios de cultura tradicionais dos laboratórios. Para isso, projetos metagenômicos são propostos para estudar comunidades microbianas presentes no meio ambiente, a partir de técnicas moleculares, em especial o seqüenciamento. Dessa forma
Publicado em: 2009
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6. Clonagem, expressão heteróloga e caracterização funcional de uma endoglicanase de Penicillium echinulatum
This work describes the pioneering molecular biology studies with the fungus Penicillium echinulatum lineage 9A02S1 (DSM 18942), which resulted in the first isolation and characterization of a cDNA corresponding to an egl1 gene, encoding a endoglucanase 1 (EGL1). The egl1 cDNA was obtained from a library constructed under induction conditions of the cellulol
Publicado em: 2009
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7. Análise da expressão gênica no dermatófito Trichophyton rubrum mimetizando a infecção in vitro: pH e diferentes fontes de carbono regulando genes / Analysis of gene expression in the dermatophyte Trichophyton rubrum during the mimetic infection in vitro: pH and carboun sources are regulating genes
Dermatophytes are a group of fungi filamentous that have the ability to invade keratinized substrates, causing dermatophytosis in humans and animals and only penetrate deeper if the host is immunocompromised. Trichophyton rubrum is an anthropophilic and cosmopolitan fungi, the most common agent of superficial mycoses, which uses cell components such as prote
Publicado em: 2008
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8. Antígenos da forma amastigota de Leishmania chagasi identificados por dupla varredura da biblioteca de cDNA
Control of human visceral leishmaniasis in endemic regions is hampered in part by the lack of knowledge with respect of the role reservoirs and vector. In addition, there is not yet an understanding of how non-symptomatic subclinical infection might influence the maintenance of infection in a particular locality. Of worrisome is the limited accessibility to
Publicado em: 2006
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9. Comparative analysis of core amino acid residues of H-2D(b)-restricted cytotoxic T-lymphocyte recognition epitopes in simian virus 40 T antigen.
Simian virus 40 (SV40) tumor (T) antigen expressed in H-2b SV40-transformed cells induces the generation of Lyt-2+ (CD8+) cytotoxic T lymphocytes (CTL), which are involved in tumor rejection, in syngeneic mice. Five CTL recognition sites on T antigen have been described by using mutant T antigens. Four of the sites (I, II, III, and V) are H-2Db restricted an
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10. Exponential increases of RNA virus fitness during large population transmissions.
The great adaptability shown by RNA viruses is a consequence of their high mutation rates. Here we investigate the kinetics of virus fitness gains during repeated transfers of large virus populations in cell culture. Results always show that fitness increases exponentially. Low fitness clones exhibit regular increases observed as biphasic periods of exponent
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11. T lymphocyte cloning from rejected human kidney allografts. Growth frequency and functional/phenotypic analysis.
Mechanically harvested lymphocytes invading an irreversibly rejected human kidney allograft were seeded at limiting dilution to calculate the frequency of growing precursors. Optimal growth frequency (1/13) was obtained when Epstein-Barr virus (EBV)-transformed donor B lymphocytes were used as stimulators (D-BLCL) in the presence of interleukin 2 (IL-2). The
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12. A novel method to isolate cells with conditional gene expression using fluorescence activated cell sorting.
An inducible expression system using control elements of the tetracycline resistance operon has recently shown promise for conditional gene expression of any gene of interest. However, intensive screening of multiple independent clones is often required to find cell lines with optimal induction characteristics. By coupling expression of the gene of interest