Organotypic Culture
Mostrando 1-12 de 46 artigos, teses e dissertações.
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1. Prolonged viability of human organotypic skin explant in culture method (hOSEC)
Abstract BACKGROUND: Currently, the cosmetic industry is overwhelmed in keeping up with the safety assessment of the increasing number of new products entering the market. To meet such demand, research centers have explored alternative methods to animal testing and also the large number of volunteers necessary for preclinical and clinical tests. OBJECTIVES:
An. Bras. Dermatol.. Publicado em: 2015-06
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2. Avaliação do potencial neuroprotetor do 17β-estradiol em fatias de cultura organotípica de hipocampo de rato submetidas à privação de oxigênio e glicose / Evaluatuion of the potential neuroprotection of 17-estradiol in organotypic slice cultures forat hippocampus exposed to oxygen and glucose deprivation
O cérebro é dependente de um fluxo sanguíneo contínuo para suplemento de glicose e oxigênio. A isquemia cerebral resulta na degeneração celular e perda da sua funcionalidade. Culturas organotípicas representam um modelo in vitro que permite estudar o dano cerebral isquêmico. Neste trabalho nós investigamos o efeito neuroprotetor do 17b-estradiol nu
Publicado em: 2010
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3. Efeito da vitamina D no perfil transcricional de cultura organotípica de câncer de mama / Vitamin D effect in the transcriptional profile of breast cancer organotypic culture
High 1,25(OH)2D3 (VD) concentrations (10-100nM) exerts antiproliferative effects and modifies gene expression profile in breast cancer (BC) cell lines. However, studies conducted in cell lines disconsider stromal-epithelium interactions, which are known to regulate breast cancer development. Besides, high VD concentrations may cause hypercalcemia in vivo. Ou
Publicado em: 2010
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4. Resveratrol-loaded nanocapsules treatment reduces in vitro and in vivo glioma growth
Glioblastoma multiforme is a devastating cerebral tumor that submits to an awful prognostic. Because of this, improvements in the treatments are necessary to decrease the side effects and to increase the survival of the patients. Resveratrol, a constituent of the red wine, has been described to inhibit carcinogenesis at multiple stages. Biodegradable nanopar
Publicado em: 2010
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5. Caracterização de metaloproteinases de matriz e reck em queratinócitos primários que expressam oncoproteínas do papilomavírus humano (HPV) / Characterization of matrix metalloproteinases and reck in primary keratinocytes that express human papillomavirus (HPV) oncoproteins
Os tumores da cérvice-uterina, que representam uma das principais doenças ginecológicas em mulheres na idade reprodutiva em todo o mundo, estão etiologicamente associados com a infecção pelo papilomavírus humano (HPV). A progressão de uma lesão intraepitelial escamosa de baixo-grau (LSIL) a um carcinoma invasivo de cérvix uterina está acompanhada
Publicado em: 2010
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6. Effect of conditioned medium of mesenchymal stem cells on the in vitro maturation and subsequent development of mouse oocyte
Mesenchymal stem cells (MSCs) secrete a variety of cytokines and growth factors in addition to self-renewal and multiple forms of differentiation. Some of these secreted bioactive factors could improve meiotic maturation in vitro and subsequent embryo developmental potential. The aim of the present study was to determine whether in vitro maturation (IVM) of
Brazilian Journal of Medical and Biological Research. Publicado em: 31/10/2008
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7. Secreção de hormônio de crescimento de camundongo por queratinícitos humanos primários: perspectivas para um modelo animal de terapia gênica cutânea / SECRETION OF MOUSE GROWTH HORMONE BY TRANSDUCED PRIMARY HUMAN KERATINOCYTES: PROSPECTS FOR AN ANIMAL MODEL OF CUTANEOUS GENE THERAPY
Keratinocytes are a very attractive vehicle for ex vivo gene transfer and systemic delivery, since proteins secreted by these cells may reach the circulation via a mechanism which mimics the natural process. An efficient retroviral vector (LXSN) encoding the mouse growth hormone gene (mGH) was used to transduce primary human keratinocytes. Organotypic raft c
Publicado em: 2008
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8. Synthesis of infectious human papillomavirus type 18 in differentiating epithelium transfected with viral DNA.
The lack of a permissive system for the propagation of viral stocks containing abundant human papillomavirus (HPV) particles has hindered the study of infectivity and the early stages of HPV replication. The organotypic (raft) culture system has permitted the study of a number of the differentiation-specific aspects of HPV, including amplification of viral D
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9. Production of infectious bovine papillomavirus from cloned viral DNA by using an organotypic raft/xenograft technique
Bovine papillomavirus type 1 (BPV-1) induces fibropapillomas in its natural host and can transform fibroblasts in culture. The viral genome is maintained as an episome within fibroblasts, which has allowed extensive genetic analyses of the viral functions required for DNA replication, gene expression, and transformation. Much less is known about BPV-1 gene e
The National Academy of Sciences.
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10. Global Effects of Human Papillomavirus Type 18 E6/E7 in an Organotypic Keratinocyte Culture System†
The effects of human papillomavirus type 18 (HPV-18) E6 and E7 proteins on global patterns of host gene expression in primary human keratinocytes grown in organotypic raft culture system were assessed. Primary human keratinocytes were infected with retroviruses that express the wild-type HPV-18 E6 and E7 genes from the native differentiation-dependent HPV en
American Society for Microbiology.
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11. Mifepristone (RU486) protects Purkinje cells from cell death in organotypic slice cultures of postnatal rat and mouse cerebellum
Mifepristone (RU486), which binds with high affinity to both progesterone and glucocorticosteroid receptors (PR and GR), is well known for its use in the termination of unwanted pregnancy, but other activities including neuroprotection have been suggested. Cerebellar organotypic cultures from 3 to 7 postnatal day rat (P3—P7) were studied to examine the
National Academy of Sciences.
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12. Human Papillomavirus E6E7-Mediated Adenovirus Cell Killing: Selectivity of Mutant Adenovirus Replication in Organotypic Cultures of Human Keratinocytes
Replication-competent adenoviruses are being investigated as potential anticancer agents. Exclusive virus replication in cancer cells has been proposed as a safety trait to be considered in the design of oncolytic adenoviruses. From this perspective, we have investigated several adenovirus mutants for their potential to conditionally replicate and promote th
American Society for Microbiology.