Ouabain Insensitive Na Atpase
Mostrando 1-11 de 11 artigos, teses e dissertações.
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1. RepercussÃo da desnutriÃÃo, durante a gestÃo, sobre o estresse oxidativo placentÃrio e transportadores de sÃdio no rim da prole de ratos adultos
A desnutriÃÃo intra-uterina tem sido correlacionada com o desenvolvimento de doenÃas cardiovasculares e renais, que estÃo vinculadas ao balanÃo de Na+ alterado. No presente estudo, investigamos se a mÃ-nutriÃÃo materna eleva o estresse oxidative placentÃrio com impacto subseqÃente nos transportadores renais de Na+ dependentes de ATP, na prole. A m�
Publicado em: 2008
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2. Sodium pumps in the Malpighian tubule of Rhodnius sp.
Malpighian tubule of Rhodnius sp. express two sodium pumps: the classical ouabain-sensitive (Na+ + K+)ATPase and an ouabain-insensitive, furosemide-sensitive Na+-ATPase. In insects, 5-hydroxitryptamine is a diuretic hormone released during meals. It inhibits the (Na+ + K+)ATPase and Na+ -ATPase activities indicating that these enzymes are involved in fluid s
Anais da Academia Brasileira de Ciências. Publicado em: 2000-09
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3. Ouabain-Uninhibited Sodium Transport in Human Erythrocytes. EVIDENCE AGAINST A SECOND PUMP
Others have concluded that a second Na “pump” (active Na outflux) exists in human erythrocytes. This second pump was said to be ouabain-insensitive, unlike the classic ouabain-sensitive Na-K pump. An alternative explanation is that “pump II” is Na exchange diffusion. These hypotheses were examined in the present experiments, utilizing 22Na influx and
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4. Isolation of diploid human lymphoblast mutants presumably homozygous for ouabain resistance
From a mass culture of diploid human lymphoblasts we have isolated a subline resistant to 1 μM ouabain, and from this, a subline resistant to 10 μM ouabain. These sublines occurred spontaneously, but similar mutants were induced with 10-fold increased frequency by treatment with mutagens, in which case they could be selected as clones in soft agarose. Scat
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5. Functional expression and segmental localization of rat colonic K-adenosine triphosphatase.
A putative cDNA for the colonic K-ATPase has recently been cloned (Crowson, M.S., and G. E. Shull. 1992. J. Biol. Chem. 267:13740-13748). Considerable evidence exists that there are two K-ATPases and active K absorptive processes in the rat distal colon: one that is ouabain sensitive and the other ouabain insensitive. The present study used the baculovirus e
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6. High-affinity ouabain binding by a chimeric gastric H+,K+-ATPase containing transmembrane hairpins M3-M4 and M5-M6 of the α1-subunit of rat Na+,K+-ATPase
Na+,K+-ATPase and gastric H+,K+-ATPase are two related enzymes that are responsible for active cation transport. Na+,K+-ATPase activity is inhibited specifically by ouabain, whereas H+,K+-ATPase is insensitive to this drug. Because it is not known which parts of the catalytic subunit of Na+,K+-ATPase are responsible for ouabain binding, we prepared ch
The National Academy of Sciences.
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7. Identification and properties of pathways for K+ transport in guinea-pig and rat alveolar epithelial type II cells.
86Rb+ was used to study potassium uptake and efflux in type II pneumocytes freshly isolated from adult guinea-pig and rat lung. Both species exhibited a substantial ouabain-sensitive component of potassium influx. In rats, most of the ouabain-resistant influx was abolished by bumetanide and removal of extracellular chloride elicited no further effect. In con
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8. Does the colonic H,K-ATPase also act as an Na,K-ATPase?
We previously have demonstrated that the colonic P-ATPase α subunit cDNA encodes an H,K-ATPase when expressed in Xenopus laevis oocytes. Besides its high level of amino acid homology (75%) with the Na,K-ATPase, the colonic H,K-ATPase also shares a common pharmacological profile with Na,K-ATPase, because both are ouabain-sensitive and Sch 28080-insensitive.
The National Academy of Sciences.
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9. Ouabain-insensitive K-adenosine triphosphatase in distal nephron segments of the rabbit.
An electrogenic H-ATpase sensitive to inhibition by N-ethyl-maleimide has been reported to be present in renal distal tubules. In contrast to another H-ATPase (gastric H-K-ATPase), the renal enzyme is not stimulated by K+ and is not inhibited by vanadate. However, our preliminary observations indicated that a K-stimulated ATPase (K-ATPase) sensitive to inhib
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10. Membrane Adenosine Triphosphatase of Escherichia coli: Activation by Calcium Ion and Inhibition by Monovalent Cations1
Membrane ghost preparations of Escherichia coli K-12 obtained by osmotic lysis of lysozyme-induced spheroplasts were found to possess both Mg++- and Ca++-activated adenosine 5′-triphosphatase (ATPase, EC 3.6.1.3) activities. Maximal activities of 1.0 to 1.5 μmoles of orthophosphate released per min per mg of protein were obtained at pH 9.0 with a molar Mg
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11. Fluorescence measurements of cytosolic free Na concentration, influx and efflux in gastric cells.
Regulation of cytosolic free Na (Nai) was measured in isolated rabbit gastric glands with the use of a recently developed fluorescent indicator for sodium, SBFI. Intracellular loading of the indicator was achieved by incubation with an acetoxymethyl ester of the dye. Digital imaging of fluorescence was used to monitor Nai in both acid-secreting parietal cell