Paracoccidioides Brasiliensis Chitinase Purification
Mostrando 1-3 de 3 artigos, teses e dissertações.
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1. Distinct chitinases are expressed during various growth phases of the human pathogen Paracoccidioides brasiliensis
The aim of this work was the partial purification and subsequent evaluation of chitinase expression during the various growth phases of Paracoccidioides brasiliensis. Initially, PbCTS1r was expressed as a recombinant protein and displayed enzymatic activity against 4-MU-[N-acetylglucosamine (GlcNAc)]3 and 4-MU-(GlcNAc)2. Two proteins, 45 kDa and 39 kDa in si
Mem. Inst. Oswaldo Cruz. Publicado em: 2012-05
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2. Purificação parcial das quitinases, Pbcts1 e Pbcts2, do fungo Paracoccidiodes brasiliensis / Partial purification of chitinases, and Pbcts1 Pbcts2, fungus Paracoccidioides brasiliensis
Paracoccidoides brasiliensis is a human pathogenic dimorphic fungus. The recombinant chitinase from P. brasiliensis, Pbcts1r, was overexpressed in Escherichia coli using pET-32a (+) as vector. The enzyme was produced as inclusion bodies and became soluble by Sarkosyl being purified by a single step using a Ni-NTA resin. Pbcts1r showed activity against 4-MU-(
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 03/04/2008
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3. Purificação parcial das quitinases, Pbcts1 e Pbcts2, do fungo Paracoccidiodes brasiliensis / Partial purification of chitinases, and Pbcts1 Pbcts2, fungus Paracoccidioides brasiliensis
Paracoccidioides brasiliensis é um fungo dimórfico patogênico humano. A quitinase recombinante de P. brasiliensis, Pbcts1r, foi superexpressa em Escherichia coli utilizando pET-32(a)+ como vetor. A enzima foi produzida em corpos de inclusão e se tornou solúvel pela adição de sarkosyl, sendo purificada em um único passo com a utilização da resina Ni
Publicado em: 2008