Pcr Ribotyping
Mostrando 1-12 de 128 artigos, teses e dissertações.
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1. The occurrence and dissemination of methicillin and vancomycin-resistant Staphylococcus in samples from patients and health professionals of a university hospital in Recife, State of Pernambuco, Brazil
Introduction Methicillin-resistant Staphylococcus aureus (MRSA) strains have been responsible for many nosocomial outbreaks. Within hospitals, colonized employees often act as reservoirs for the spread of this organism. This study collected clinical samples of 91 patients admitted to the intensive care unit (ICU), hemodialysis/nephrology service and surgica
Rev. Soc. Bras. Med. Trop.. Publicado em: 2014-08
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2. Epidemiologia molecular e resistência a antimicrobianos de amostras de Salmonella spp, isoladas de frangos e perus no período de 2005 e 2006. / Molecular epidemiology and antimicrobial resistance of Salmonella spp, isolated from chicken and turkey between 2005 and 2006
The objective of the study was to evaluate antimicrobial resistance and to characterize the sorovars of Salmonella spp. Isolates from the Programa de Redução de Patógenos (PRP) - Ministério da Agricultura do Brazil. Overall we analysed 1781 Salmonella spp. isolates from chicken broiler and turkey from 2005 to 2009 at different slaughter-houses and certif
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 30/03/2011
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3. Antimicrobial resistance and PCR-ribotyping of Shigella responsible for foodborne outbreaks occurred in southern Brazil
Little information about Shigella responsible for foodborne shigellosis is available in Brazil. The present study aimed to investigate the antimicrobial resistance and PCR-ribotyping patterns of Shigella isolates responsible for foodborne outbreaks occurred in Rio Grande do Sul State (RS), Southern Brazil in the period between 2003 and 2007. Shigella strains
Brazilian Journal of Microbiology. Publicado em: 2010-12
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4. Molecular typing and biological characteristics of Pseudomonas aeruginosa isolated from cystic fibrosis patients in Brazil
The present study had as objective to evaluate the genotypic diversity and biological characteristics, such as hemolysin, protease, elastase of 56 clinical strains of Pseudomonas aeruginosa isolated from 13 cystic fibrosis (CF) patients attending at the School Hospital of Campinas State University (UNICAMP), Brazil. Genotypic diversity has been determined by
Brazilian Journal of Infectious Diseases. Publicado em: 2010-10
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5. Isolamento, identificação e caracterização de Shigella spp. envolvidas em surtos alimentares no Rio Grande do Sul / Isolation, identification, and characterization of Shigella spp. associated with foodborne outbreaks occurred in Rio Grande do Sul State, Brazil
No Brasil existem poucos relatos sobre casos de Shigelose, um fator que contribui com o pouco conhecimento a respeito dessa doença. Além disso, a falta de obrigatoriedade por parte da legislação vigente em relação à pesquisa de Shigella tem contribuído com este fato. O presente trabalho teve por objetivo isolar, identificar e caracterizar isolados de
Publicado em: 2009
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6. Ribotyping, biotyping and capsular typing of Haemophilus influenzae strains isolated from patients in Campinas, southeast Brazil
Forty-five Haemophilus influenzae strains isolated from patients were characterized based on biochemical characteristics. Their capsular types were determined by polymerase chain reaction (PCR); they were compared, using two molecular methods [ribotyping with a specific DNA probe amplified from the 16S rDNA region from H. influenzae and through restriction f
Brazilian Journal of Infectious Diseases. Publicado em: 2008-10
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7. Comparison of PCR-based molecular markers for the characterization of Proteus mirabilis clinical isolates
Proteus mirabilis is one of the most important pathogens associated with complicated urinary tract infections (acute pyelonephritis, bladder infections, kidney stones) and bacteremia, affecting patients with anatomical abnormalities, immunodeficiency, and long-term urinary catheterization. For epidemiological purposes, various molecular typing methods, such
Brazilian Journal of Infectious Diseases. Publicado em: 2008-10
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8. Application of PCR ribotyping and tDNA-PCR for Klebsiella pneumoniae identification
PCR analysis of 16S-23S internal transcribed spacer (PCR ribotyping) and tRNA intergenic spacer (tDNA-PCR) were evaluated for their effectiveness in identification of clinical strains of Klebsiella pneumoniae and differentiation with related species. For this purpose both methods were applied to forty-three clinical isolates biochemically identified as K. pn
Memórias do Instituto Oswaldo Cruz. Publicado em: 30/10/2007
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9. Bacillus cereus isolation, ribotyping and control in post-pasteurized milk / Isolamento, ribotipagem e controle de Bacillus cereus após a pasteurização do leite
Bacillus cereus recontamination of post-pasteurized milk was assessed by product analysis and by previously sanitized surfaces analysis. The milk samples were collected during nine weeks and the surfaces of the packaging machine screw (PMS); of the packaging machine leveling tank (PMLT); of the package forming tube (PFT); of the packaging tube (PT), and of t
Publicado em: 2007
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10. Rastreamento de Listeria monocytogenes em industrias processadoras de queijo frescal tipo latino, nos Estados Unidos da America, empregando a subtipagem molecular . / Tracking of would listeria monocytogenes in processing industries of frescal cheese Latin type, in the United States of America, using the molecular subtipagem.
Latin-style fresh cheeses, which have been linked to at least two human listeriosis outbreaks in the US, are considered to be high risk foods for Listeria monocytogenes contamination. We evaluated L. monocytogenes contamination patterns in three Latin-style fresh cheese processing plants to gain a better understanding of L. monocytogenes contamination source
Publicado em: 2004
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11. PCR-based ribotyping of Staphylococcus aureus
Genotyping techniques are valuable tools for the epidemiologic study of Staphylococcus aureus infections in the hospital setting. Pulsed-field gel electrophoresis (PFGE) is the current method of choice for S. aureus strain typing. However, the method is laborious and requires expensive equipment. In the present study, we evaluated the natural polymorphism of
Brazilian Journal of Medical and Biological Research. Publicado em: 2002-02
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12. AnÃlise das regiÃes espaÃadoras intergÃnicas do rRNA 16S-23S em diferentes gÃneros bacterianos
Bacterial ribosomes carry three types of rRNA: 23S, 16S and 5S encoded in genes organized in operons separated by intergenic spacer regions (ISRs) containing one or more tRNA genes. The genetic information derived from the rRNA operon provides a valuable taxonomic information, since the ISRs, especially those located between the 16S and 23S regions of the rD
Publicado em: 2002