Pectin Liase
Mostrando 1-6 de 6 artigos, teses e dissertações.
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1. Experimental Design Applied to the Optimization and Partial Characterization of Pectin Liase from a Newly Isolated Penicillium brasilianum
Penicillium brasilianum was previously isolated from tea and identified by molecular biology technique. A Plackett-Burman design, followed by a complete second order design was used for the screening of most important factors and to maximize the pectin liase (PMGL) activity, respectively. The maximum PMGL activity by P. brasilianum achieved was 9.0 U/mL afte
Braz. arch. biol. technol.. Publicado em: 2014-12
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2. Caracterização de linhagens recombinantes de Penicillium griseoroseum: análise do cariótipo, da expressão dos genes plg 1 e pgg 2 e da produção de pectina liase e poligalacturonase em resposta ao pH do meio / Characterization of recombinant strains of Penicillium griseoroseum: karyotype analysis, expression of plg1 and pgg2 genes and production of pectin lyase and polygacturonase in response to medium pH
Os estudos genéticos e fisiológicos realizados com o isolado Penicillium griseoroseum CCT6421 ressaltam as vantagens para a sua aplicação como hospedeiro para produção de proteínas. A obtenção e análise de linhagens recombinantes de P. griseoroseum com alta produção de pectina liase (PL) e poligalacturonase (PG) evidenciaram o eficiente sistema d
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 23/05/2011
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3. Obtenção e caracterização de linhagens recombinantes de Penicillium griseoroseum com alta produção de pectina liase e poligalacturonase / Obtainment and characterizing of recombinant strains of Penicillium griseoroseum with pectin lyase and polygalacturonase overproduction
Penicillium griseoroseum has been described as a promising species for polygalacturonase (PG) and pectin lyase (PL) production. However, the genes encoding these enzymes in this organism require induction by pectin and are repressed by glucose. One strategy to increase the expression of these genes is the replacement of original promoter by a strong constitu
Publicado em: 2007
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4. Regulation of the expression of the gene plg1 that code for pectin lyase in Penicillium griseoroseum / Regulação da expressão do gene plg1 que codifica pectina liase em Penicillium griseoroseum
Penicillium griseoroseum has been considered a promising species for industrial application because of its capacity to produce pectin lyase (PL) and polygalacturonase. When P. griseoroeum is grown in the presence of glucose, plg1 gene transcripts are detected at low levels, evidencing the effects of catabolic repression. However, in the absence of the natura
Publicado em: 2006
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5. Isolamento, caracterização e regulação de genes que codificam pectato liase em Crinipellis perniciosa, agente etiológico da vassoura-de-bruxa do cacaueiro (Theobroma cacao) / Isolation, characterization and regulation of genes encoding pectate lyase in Crinipellis perniciosa, etiological agent of the witches broom in cocoa (Theobroma cacao)
In this work, genes encoding the pectinolytic enzyme pectate lyase were isolated from the genome of the fungus Crinipellis perniciosa, etiological agent of the witches broom disease of cocoa. Pectinolytic enzymes have been correlated with the pathogenicity of some phytopathogenic fungi. Preliminary database searches of the Genome Project Witches broom, revea
Publicado em: 2006
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6. Organization of pectin lyase encoding gene from Penicillium expansum and isolation of recombinant strains of Penicillium griseoroseum with high pectin lyase production / Organização do gene de pectina liase em Penicillium expansum e obtenção de linhagens recombinantes de Penicillium griseoroseum com alta produção de pectina liase
A seqüência de nucleotídeos contendo o gene ple1 que codifica pectina liase em Penicillium expansum foi clonada. Esta seqüência contém 874 nucleotídeos da região promotora, 1342 nucleotídeos da região codificadora e 469 nucleotídeos da região terminadora. A região codificadora do gene ple1 é interrompida por dois íntrons, de 101 e 116 nucleot�
Publicado em: 2004