Pet Vector
Mostrando 1-12 de 82 artigos, teses e dissertações.
-
1. Improved antifungal activity of barley derived chitinase I gene that overexpress a 32 kDa recombinant chitinase in Escherichia coli host
Abstract Agricultural crops suffer many diseases, including fungal and bacterial infections, causing significant yield losses. The identification and characterisation of pathogenesis-related protein genes, such as chitinases, can lead to reduction in pathogen growth, thereby increasing tolerance against fungal pathogens. In the present study, the chitinase I
Braz. J. Microbiol.. Publicado em: 2018-06
-
2. Support vector machine-based classification of neuroimages in Alzheimer’s disease: direct comparison of FDG-PET, rCBF-SPECT and MRI data acquired from the same individuals
Objective: To conduct the first support vector machine (SVM)-based study comparing the diagnostic accuracy of T1-weighted magnetic resonance imaging (T1-MRI), F-fluorodeoxyglucose-positron emission tomography (FDG-PET) and regional cerebral blood flow single-photon emission computed tomography (rCBF-SPECT) in Alzheimer’s disease (AD). Method: Brain T1-MR
Rev. Bras. Psiquiatr.. Publicado em: 02/10/2017
-
3. Expression, Purification and Functional Assessment of Smallest Isoform of Human Interleukin-24 in Escherichia coli
ABSTRACT Interleukin-24 (IL-24) is a novel tumor-suppressor gene that has different alternative splice isoforms. It has been shown that new smallest isoform of human IL-24 gene, lacking three exons, induces higher levels of cytotoxicity than all the isoforms, indicating shortest isoform of IL-24 may be a new promising anti-cancer agent. In this study, we aim
Braz. arch. biol. technol.. Publicado em: 07/08/2017
-
4. Expression of cry1Ab gene from a novel Bacillus thuringiensis strain SY49-1 active on pest insects
ABSTRACT In this study, the cry1Ab gene of previously characterized and Lepidoptera-, Diptera-, and Coleoptera-active Bacillus thuringiensis SY49-1 strain was cloned, expressed and individually tested on Ephestia kuehniella (Lepidoptera: Pyralidae) and Plodia interpunctella (Lepidoptera: Pyralidae) larvae. pET-cry1Ab plasmids were constructed by ligating the
Braz. J. Microbiol.. Publicado em: 2016-09
-
5. A comparison between the recombinant expression and chemical synthesis of a short cysteine-rich insecticidal spider peptide
Abstract Background: The choice between heterologous expression versus chemical synthesis for synthesizing short cysteine-rich insecticidal peptides from arthropods may impact the obtainment of yields and well-folded bioactive molecules for scientific research. Therefore, two recombinant expression systems were compared to
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 11/08/2015
-
6. Characterization and expression analysis of WOX2 homeodomain transcription factor in Aegilops tauschii
The WUSCHEL (WUS)-related homeobox (WOX) gene family coordinates transcription during the early phases of embryogenesis. In this study, a putative WOX2 homolog was isolated and characterized from Aegilops tauschii, the donor of D genome of Triticum aestivum. The sequence consisted of 2045 bp, and contained an open reading frame (ORF), encoded 322 amino acids
Genet. Mol. Biol.. Publicado em: 2015-03
-
7. Induction of protective T-helper 1 immune responses against Echinococcus granulosus in mice by a multi-T-cell epitope antigen based on five proteins
In this study, we designed an experiment to predict a potential immunodominant T-cell epitope and evaluate the protectivity of this antigen in immunised mice. The T-cell epitopes of the candidate proteins (EgGST, EgA31, Eg95, EgTrp and P14-3-3) were detected using available web-based databases. The synthesised DNA was subcloned into the pET41a+ vector and ex
Mem. Inst. Oswaldo Cruz. Publicado em: 2013-06
-
8. Clonagem molecular do gene ipaC de Shigella sp e estudo da expressão por Lactococcus lactis / Molecular cloning and expression analysis in Lactococcus lactis of ipaC gene from Shigella sp
Shigellosis is a severe intestinal infection caused by Gram-negative bacteria which belongs to the genus Shigella. It is a pathology with high incidence and it is known that 99% of cases occur in underdevelopment countries. Among these cases, 69% of them affect children under 5 years old. The high incidence of Shigella in underdevelopment countries, is mainl
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 30/05/2012
-
9. Vacinas recombinantes contra erisipela suína: desenvolvimento integrado de bioprocesso, da biologia molecular ao biorreator
A erisipela suína é uma das enfermidades que causam grandes prejuízos na suinocultura em todo o mundo. A doença é causada pela bactéria Erysipelothrix rhusiopathiae, e a proteína de superfície SpaA desse microrganismo é um de seus principais antígenos. Neste trabalho, estudou-se o desenvolvimento de vacinas recombinantes contra a erisipela suína a
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 11/10/2011
-
10. Produção recombinante e caracterização de uma desintegrina da peçonha de Rhinocerophis alternatus (antiga Bothrops alternatus) com atividade antimetastática visando o desenvolvimento de um novo fármaco
This work presents the study of the effects of an RGD disintegrin from Rhinocerophis alternatus (ancient Bothrops alternatus) in key steps of the metastatic cascade. Disintegrins are cysteine-rich peptides, which have several biological activities related to cell-cell interaction and cell adhesion. The correct pairing of disulfide bonds exposes the adhesive
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 28/06/2011
-
11. Expressão heteróloga de neurotoxinas do veneno da aranha Phoneutria nigriventer
The venom of the spider Phoneutria nigriventer contains several peptides that act on ion channels and receptors of the nervous system of insects and mammals. The toxins Tx3-6 and Tx3-4 are voltage-gated calcium channel blockers and have shown a pharmacological potential in the treatment of pain and in neuroprotection. Only small amounts of these toxins can b
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 17/06/2011
-
12. Expression of mouse beta defensin 2 in Escherichia coli and its broad-spectrum antimicrobial activity
Mature mouse beta defensin 2 (mBD2) is a small cationic peptide with antimicrobial activity. Here we established a prokaryotic expression vector containing the cDNA of mature mBD2 fused with thioredoxin (TrxA), pET32a-mBD2. The vector was transformed into Escherichia Coli (E. coli) Rosseta-gami (2) for expression fusion protein. Under the optimization of fer
Brazilian Journal of Microbiology. Publicado em: 2011-09