Pg Amp1
Mostrando 1-12 de 37 artigos, teses e dissertações.
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1. ATIVIDADE ANTIMICROBIANA DO Pg-AMP1 RECOMBINANTE, UM PEPTÍDEO RICO EM GLICINA, ISOLADO DE GOIABA (Psidium guajava L.)
The search for new antibiotics of broad spectrum of activity has increased in recent decades due to the increasing number of bacteria resistant to conventional antibiotics. The Pg-AMP1 recombinant peptide expressed in a heterologous system in Escherichia coli, strains showed antibacterial activity against Gram-positive and Gram-negative. From the sequence of
Publicado em: 2009
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2. Separação de ampicilina produzida enzimaticamente por reação entre éster metílico de fenilglicina e ácido 6-aminopenicilânico.
The separation/concentration process of the products obtained from the enzymatic synthesis of ampicillin (AMP) catalyzed by immobilized penicillin G acylase was the focus of this Thesis. Hydrophobic resin adsorption and isoelectric precipitation were the processes herein evaluated. The antibiotic was produced in the solid phase, from 6-aminopenicillanic acid
Publicado em: 2003
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3. Prostacyclin modulates cholesteryl ester hydrolytic activity by its effect on cyclic adenosine monophosphate in rabbit aortic smooth muscle cells.
We tested the hypothesis that prostacyclin (PGI2), 6-keto-prostaglandinF1 alpha(6-keto-PGF1 alpha), and several E series prostaglandins (PG) may affect the activity of cholesteryl ester (CE) hydrolase since our previous experiments indicated that smooth muscle cells (SMC) in neointima of injured rabbit aorta (a) acquire the capacity to produce PGI2 and (b) h
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4. Copper amplification of prostaglandin E2 stimulation of the release of luteinizing hormone-releasing hormone is a postreceptor event.
We have shown that copper amplifies prostaglandin E2 (PGE2) stimulation of luteinizing hormone-releasing hormone (LH-RH) from explants of the median eminence area (MEA) and that this process is calcium-dependent. Since a Ca-cAMP pathway has been implicated in PGE2 action on the LH-RH neuron, in this study we wished to ascertain if copper exerts its effect on
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5. Role of Renal Prostaglandins in Sympathetically Mediated Renin Release in the Rat
Renal prostaglandins (PG) appear to mediate renin release due to stimulation of the intrarenal baroreceptor, but not that due to activation of the macula densa. However, as the role of PG in sympathetically mediated renin release remains unclear, a possible interrelationship between these factors was examined in conscious rats. Hydralazine increased the seru
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6. Role of local prostaglandin synthesis in the modulation of proliferative activity of rat colonic epithelium.
The role of local prostaglandin (PG) synthesis in the modulation of the proliferative activity of colonic epithelium was examined in rat colon. Experimental rats were given either indomethacin (5 mg/kg s.c. every 8 h for three doses) or aspirin (0.5 g/100 g diet for 3 d). In rats treated with indomethacin or aspirin, the incorporation of [3H]thymidine (dThd)
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7. The Effects of the Immunologic Release of Histamine upon Human Lung Cyclic Nucleotide Levels and Prostaglandin Generation
The effect of the antigen-induced, immunoglobulin (Ig)E-dependent release of mediators from human lung tissue was analyzed for coincident changes in the tissue levels of cyclic nucleotides. Simultaneously with the appearance of mediators, lung cyclic guanosine 3′,5′-monophosphate (GMP) increased from 0.9±0.2 to 12.63±4.5 pmol/mg protein and cyclic AMP
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8. Regulation of endothelial cell cyclic nucleotide metabolism by prostacyclin.
An analysis of prostaglandin-stimulated adenosine 3',5'-cyclic monophosphate (cyclic AMP) accumulation in cultured human umbilical vein endothelial cells showed prostacyclin (PGI2) to be the most potent agonist followed by prostaglandin (PG)H2, which was more potent than PGE2, while PGD2 was essentially inactive. The endothelial cells studied apparently have
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9. Covalent modification of proteins by ligands of steroid hormone receptors.
Retinoylation, acylation with retinoic acid (RA), is a covalent modification of proteins occurring in a variety of eukaryotic cell lines. In this study, we found that proteins in HL-60 cells were labeled by 17 beta-[3H]estradiol (E2), [3H]progesterone (Pg), 1 alpha,25-dihydroxy[3H]vitamin D3 [1,25(OH)2D3], [125I]triiodothyronine (T3), [125I]thyroxine (T4), a
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10. Impaired phosphorus conservation and 1,25 dihydroxyvitamin D generation during phosphorus deprivation in familial hypophosphatemic rickets.
The pathogenesis of familial hypophosphatemic rickets (FHR) is incompletely understood. We therefore examined the effects of acute dietary phosphorus deprivation to see whether renal phosphate conservation and increased 1,25 dihydroxyvitamin D [1,25(OH)2D] plasma levels, which normally follow restriction of phosphorus intake, could be induced in patients wit
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11. Prostaglandin E Modulation of the Mitogenic Response of Human T Cells: DIFFERENTIAL RESPONSE OF T-CELL SUBPOPULATIONS
Prostaglandins (PG) of the E series, PGE1 and PGE2 (PGEs), can induce elevations of intracellular cyclic AMP (cAMP) among thymus-derived (T) lymphocytes (T cells) and inhibit their reactivity. For example, 0.1 μM of PGEs induces a two- to threefold increase of intracellular cAMP among human peripheral blood T cells and a 20-30% suppression of their blastoge
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12. Automatic and manual latex agglutination tests for measurement of cholera toxin and heat-labile enterotoxin of Escherichia coli.
Automated and manual latex agglutination methods were employed to measure cholera toxin (CT), heat-labile enterotoxin (LT) of Escherichia coli, and their subunits A and B. Dow polystyrene latex particles (diameter, 0.22 microns) and polystyrene-chlorostyrene latex particles (diameter, 1 micron) were sensitized by rabbit-specific immunoglobulin for each antig