Pghs
Mostrando 1-12 de 24 artigos, teses e dissertações.
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1. Docking molecular aplicado ao estudo da formação de complexos entre análogos de resveratrol e derivados de 1,2,3-triazol e a enzima COX-2 / Molecular docking applied to the study of complexes formation between resveratrol analogues and 1,2,3-triazole derivatives and the COX-2 enzyme
Prostaglandinas H sintases (PGHS), ou ciclooxigenases (COX), existem em pelo menos duas isoformas, COX-1 e COX-2, codificadas por genes diferentes. A COX desempenha um papel central no processo inflamatório através da conversão do ácido araquidônico, liberado a partir dos fosfolipídios da membrana, em prostanóides bioativos. Anti-inflamatórios não e
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 14/12/2011
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2. Caracterização estrutural e conformacional de prostaglandina endoperóxido sintases
Prostaglandina Endoperóxido Sintases (PGHSs) são enzimas homodiméricas integrais de membrana, N-glicosiladas, localizadas no lúmen do retículo endoplasmático, onde catalisam o primeiro passo na síntese de prostanóides, produzindo prostaglandina H2 a partir do ácido araquidônico (AA). Constituem-se, assim, em um dos principais alvos terapêuticos no
Publicado em: 2011
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3. Interação do cininogênio humano de alta massa molecular com a superfície celular: envolvimento dos processos de endocitose e proteólise / High molecular weight human kininogen interact with cell surface: Involvement endocytosis and proteolysis process
A angiogênese e a hemostasia estão entre as mais consistentes respostas do hospedeiro associadas ao câncer, e essas duas vias interrelacionam-se, com a coagulação sanguínea e a fibrinólise influenciando a angiogênese tumoral diretamente e contribuindo com o crescimento do tumor. O fato dos tumores serem dependentes do suprimento sanguíneo tem inspir
Publicado em: 2010
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4. Análise de glicosaminoglicanos e proteoglicanos em linhagens tumorais de próstata / Analysis of glycosaminoglycans and proteoglycans in prostate cancer cell lines
Proteoglycans (PG) are cell surface, extracellular matrix and citoplasmatic granule macromolecules, that play an important role in cellcell and cell-matrix interactions. Several studies indicated that significant changes in PG content occur in the tumor stroma of epithelial neoplasms and that these alterations influence tumor growth and invasion. In this stu
Publicado em: 2010
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5. Allergic lung responses are increased in prostaglandin H synthase–deficient mice
To investigate the function of prostaglandin H synthase-1 and synthase-2 (PGHS-1 and PGHS-2) in the normal lung and in allergic lung responses, we examined allergen-induced pulmonary inflammation and airway hyperresponsiveness in wild-type mice and in PGHS-1–/– and PGHS-2–/– mice. Among nonimmunized saline-exposed groups, we found no significant diff
American Society for Clinical Investigation.
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6. Enhanced prostacyclin synthesis in endothelial cells by retrovirus-mediated transfer of prostaglandin H synthase cDNA.
A retroviral vector (BAG) was used to transfer human prostaglandin H synthase (PGHS-1) gene into a human endothelial cell line for enhancement of PGI2 synthesis. Cells infected with BAG containing PGHS-1 cDNA in the sense orientation relative to the retroviral promoter (PGHS(S)) expressed a 30-fold increase in mRNA but, due to a reading frame shift, did not
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7. Prostaglandin G/H synthase-2 is required for maximal formation of osteoclast-like cells in culture
We examined the effect on osteoclast formation of disrupting the prostaglandin G/H synthase genes PGHS-1 and-2. Prostaglandin E2 (PGE2) production was significantly reduced in marrow cultures from mice lacking PGHS-2 (PGHS-2–/–) compared with wild-type (PGHS-2+/+) cultures. Osteoclast formation, whether stimulated by 1,25-dihydroxyvitamin D3 (1,25-D) or
American Society for Clinical Investigation.
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8. Determinants of the cellular specificity of acetaminophen as an inhibitor of prostaglandin H2 synthases
Acetaminophen has antipyretic and analgesic properties yet differs from the nonsteroidal antiinflammatory drugs and inhibitors of prostaglandin H synthase (PGHS)-2 by exhibiting little effect on platelets or inflammation. We find parallel selectivity at a cellular level; acetaminophen inhibits PGHS activity with an IC50 of 4.3 μM in interleukin (IL)-1α-sti
The National Academy of Sciences.
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9. Hypercalcemia stimulates expression of intrarenal phospholipase A2 and prostaglandin H synthase-2 in rats. Role of angiotensin II AT1 receptors.
In chronic hypercalcemia, inhibition of thick ascending limb sodium chloride reabsorption is mediated by elevated intrarenal PGE2. The mechanisms and source of elevated PGE2 in hypercalcemia are not known. We determined the effect of hypercalcemia on intrarenal expression of cytosolic phospholipase A2 (cPLA2), prostaglandin H synthase-1 (PGHS-1), and prostag
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10. Lipopolysaccharide induces prostaglandin H synthase-2 protein and mRNA in human alveolar macrophages and blood monocytes.
We and others have previously demonstrated that human alveolar macrophages produce more PGE2 in response to lipopolysaccharide (LPS) than do blood monocytes. We hypothesized that this observation was due to a greater increase in prostaglandin H synthase-2 (PGHS-2) enzyme mass in the macrophage compared to the monocyte. To evaluate this hypothesis, alveolar m
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11. Interferon gamma induces prostaglandin G/H synthase-2 through an autocrine loop via the epidermal growth factor receptor in human bronchial epithelial cells.
The induction of prostaglandin G/H synthase (PGHS; prostaglandin endoperoxide synthase, cyclooxygenase) by proinflammatory cytokines accounts, at least in part, for the altered eicosanoid biosynthesis in inflammatory diseases. In secondary cultures of normal human bronchial epithelial cells (NHBECs), interferon-gamma (IFN-gamma, 10 ng/ml for 24 h) increased
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12. Ovariectomy enhances and estrogen replacement inhibits the activity of bone marrow factors that stimulate prostaglandin production in cultured mouse calvariae.
To examine PG production in estrogen deficiency, we studied effects on cultured neonatal mouse calvariae of bone marrow supernatants (MSup) from sham-operated (SHAM), ovariectomized (OVX), or 17 beta-estradiol (OVX+E)-treated mice. MSups were obtained 3 wk after OVX when bone density had decreased significantly. 10-60% MSup increased medium PGE2 and levels o