Pgip
Mostrando 1-10 de 10 artigos, teses e dissertações.
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1. Caracterização da expressão de inibidores de poligalacturonases (PGIPs) em resposta aos estresses biótico e abiótico em plantas de feijão comum (Phaseolus vulgaris L.) / Characterization of the expression of polygalacturonase inhibitors (PGIPs) in response to biotic and abiotic stresses in plants of common bean (Phaseolus vulgaris L.)
Sclerotinia sclerotiorum (Lib.) de Bary is a necrotrophic fungal pathogen that causes the disease known as white mold in many plants. During infection, it secretes several endopolygalacturonases (PGs) to degrade cell wall pectin. To counteract the action of PGs, plants express polygalacturonases-inhibiting proteins (PGIPs) that reduce the hydrolytic activity
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 26/11/2011
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2. Caracterização da expressão de inibidores de poligalacturonases (PGIPs) em resposta aos estresses biótico e abiótico em plantas de feijão comum (Phaseolus vulgaris L.) / Characterization of the expression of polygalacturonase inhibitors (PGIPs) in response to biotic and abiotic stresses in plants of common bean (Phaseolus vulgaris L.)
Sclerotinia sclerotiorum (Lib.) de Bary é um fungo necrotrófico patogênico causador da doença conhecida como mofo branco em muitas plantas. Durante a infecção, secreta várias endopoligalacturonases (endo-PGs) que degradam a pectina da parede celular da planta hospedeira. Para contrapor à ação das PGs, as plantas produzem proteínas inibidoras de po
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 26/11/2011
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3. Characterization of the dry bean polygalacturonase-inhibiting protein (PGIP) gene family during Sclerotinia sclerotiorum (Sclerotiniaceae) infection.
Polygalacturonase-inhibiting proteins are leucine-rich repeat proteins that inhibit fungal endopolygalacturonases. The interaction of polygalacturonase-inhibiting protein with endopolygalacturonases limits the destructive potential of endopolygalacturonases and may trigger plant defense responses induced by oligogalacturonides. We examined the expression of
Genetics and Molecular Research. Publicado em: 2011
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4. Tandemly Duplicated Arabidopsis Genes That Encode Polygalacturonase-Inhibiting Proteins Are Regulated Coordinately by Different Signal Transduction Pathways in Response to Fungal Infection
Polygalacturonase-inhibiting proteins (PGIPs) are plant proteins that counteract fungal polygalacturonases, which are important virulence factors. Like many other plant defense proteins, PGIPs are encoded by gene families, but the roles of individual genes in these families are poorly understood. Here, we show that in Arabidopsis, two tandemly duplicated PGI
American Society of Plant Biologists.
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5. Structural requirements of endopolygalacturonase for the interaction with PGIP (polygalacturonase-inhibiting protein)
To invade a plant tissue, phytopathogenic fungi produce several cell wall-degrading enzymes; among them, endopolygalacturonase (PG) catalyzes the fragmentation and solubilization of homogalacturonan. Polygalacturonase-inhibiting proteins (PGIPs), found in the cell wall of many plants, counteract fungal PGs by forming specific complexes with them. We report t
The National Academy of Sciences.
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6. Purification and Characterization of a Polygalacturonase-Inhibiting Protein from Phaseolus vulgaris L. 1
Homogeneous endo-polygalacturonase (PG) was covalently bound to cyanogen-bromide-activated Sepharose, and the resulting PG-Sepharose conjugate was utilized to purify, by affinity chromatography, a protein from Phaseolus vulgaris hypocotyls that binds to and inhibits PG. Isoelectric focusing of the purified PG-inhibiting protein (PGIP) showed a major protein
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7. Characterization of the Complex Locus of Bean Encoding Polygalacturonase-Inhibiting Proteins Reveals Subfunctionalization for Defense against Fungi and Insects1
Polygalacturonase-inhibiting proteins (PGIPs) are extracellular plant inhibitors of fungal endopolygalacturonases (PGs) that belong to the superfamily of Leu-rich repeat proteins. We have characterized the full complement of pgip genes in the bean (Phaseolus vulgaris) genotype BAT93. This comprises four clustered members that span a 50-kb region and, based o
American Society of Plant Biologists.
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8. The crystal structure of polygalacturonase-inhibiting protein (PGIP), a leucine-rich repeat protein involved in plant defense
Polygalacturonase-inhibiting proteins (PGIPs) are plant cell wall proteins that protect plants from fungal invasion. They interact with endopolygalacturonases secreted by phytopathogenic fungi, inhibit their enzymatic activity, and favor the accumulation of oligogalacturonides, which activate plant defense responses. PGIPs are members of the leucine-rich
National Academy of Sciences.
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9. Targeted Modification of Homogalacturonan by Transgenic Expression of a Fungal Polygalacturonase Alters Plant Growth1
Pectins are a highly complex family of cell wall polysaccharides comprised of homogalacturonan (HGA), rhamnogalacturonan I and rhamnogalacturonan II. We have specifically modified HGA in both tobacco (Nicotiana tabacum) and Arabidopsis by expressing the endopolygalacturonase II of Aspergillus niger (AnPGII). Cell walls of transgenic tobacco plants showed a 2
American Society of Plant Biologists.
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10. Use of homologous expression-secretion signals and vector-free stable chromosomal integration in engineering of Lactobacillus plantarum for alpha-amylase and levanase expression.
The genuine alpha-amylase gene from Bacillus licheniformis (amyL) is not expressed in Lactobacillus plantarum, but replacement of the amyL promoter by a strong L. plantarum promoter leads to efficient expression of the gene and secretion of more than 90% of the alpha-amylase into the culture supernatant. A series of L. plantarum genetic cassettes (transcript