Phenylmethylsulfonyl Fluoride
Mostrando 1-12 de 166 artigos, teses e dissertações.
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1. In vitro characterization of jellyfish venom fibrin(ogen)olytic enzymes from Nemopilema nomurai
Abstract Background: Because jellyfish are capable of provoking envenomation in humans, they are considered hazardous organisms. Although the effects of their toxins are a matter of concern, information on the venom components, biological activity and pathological mechanisms are still scarce. Therefore, the aim of the present study was to investigate a seri
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 08/02/2018
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2. Process optimization for production and purification of a thermostable, organic solvent tolerant lipase from Acinetobacter sp. AU07
ABSTRACT The purpose of this study was to isolate, purify and optimize the production conditions of an organic solvent tolerant and thermostable lipase from Acinetobacter sp. AU07 isolated from distillery waste. The lipase production was optimized by response surface methodology, and a maximum production of 14.5 U/mL was observed at 30 ºC and pH 7, using a
Braz. J. Microbiol.. Publicado em: 2016-09
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3. Characterization of biotechnologically relevant extracellular lipase produced by Aspergillus terreus NCFT 4269.10
Abstract Enzyme production by Aspergillus terreus NCFT 4269.10 was studied under liquid static surface and solid-state fermentation using mustard oil cake as a substrate. The maximum lipase biosynthesis was observed after incubation at 30 °C for 96 h. Among the domestic oils tested, the maximum lipase biosynthesis was achieved using palm oil. The crude lipa
Braz. J. Microbiol.. Publicado em: 2016-03
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4. Production and partial characterization of serine and metallo peptidases secreted by Aspergillus fumigatus Fresenius in submerged and solid state fermentatio
Enzyme production varies in different fermentation systems. Enzyme expression in different fermentation systems yields important information for improving our understanding of enzymatic production induction. Comparative studies between solid-state fermentation (SSF) using agro-industrial waste wheat bran and submerged fermentation (SmF) using synthetic media
Braz. J. Microbiol.. Publicado em: 2013
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5. Effects of genetic and environmental alterations on the birefringence of dental enamel organic matrix / Efeitos de alterações geneticas e ambientais sobre a birrefringencia da matriz organica do esmalte dentario
O esmalte envolve a porção coronária dos dentes e constitui a estrutura mais mineralizada do corpo vertebrado. Seu desenvolvimento tem início com secreção, processamento proteolítico e auto-agregação de uma complexa mistura de proteínas. O estabelecimento de uma matriz orgânica ordenada parece ser fundamental para a formação adequada da fase min
Publicado em: 2008
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6. Esterase profile in a pyrethroid-resistant Brazilian strain of the cattle tick Boophilus microplus (Acari, Ixodidae)
The cattle tick Boophilus microplus causes great damage in livestock and is considered one of the most important tropical ectoparasites. The traditional method of control is based on the intensive use of pesticides, however the indiscriminate use of these compounds over the years has led to the selection of resistant ticks. Hydrolases, particularly esterases
Genetics and Molecular Biology. Publicado em: 2005-12
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7. Perspectives of digestive pest control with proteinase inhibitors that mainly affect the trypsin-like activity of Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae)
The present study describes the main characteristics of the proteolytic activities of the velvetbean caterpillar, Anticarsia gemmatalis Hübner, and their sensitivity to proteinase inhibitors and activators. Midguts of last instar larvae reared on an artificial diet were homogenized in 0.15 M NaCl and centrifuged at 14,000 g for 10 min at 4ºC and the supern
Brazilian Journal of Medical and Biological Research. Publicado em: 2005-11
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8. A continuous fluorescent assay for the determination of plasma and tissue angiotensin I-converting enzyme activity
A continuous assay using internally quenched fluorescent peptides with the general sequence Abz-peptidyl-(Dnp)P-OH (Abz = ortho-aminobenzoic acid; Dnp = 2,4-dinitrophenyl) was optimized for the measurement of angiotensin I-converting enzyme (ACE) in human plasma and rat tissues. Abz-FRK(Dnp)P-OH, which was cleaved at the Arg-Lys bond by ACE, was used for the
Brazilian Journal of Medical and Biological Research. Publicado em: 2005-06
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9. The effect of growth and starvation on the lysis of the ruminal cellulolytic bacterium Fibrobacter succinogenes.
Growing cultures of Fibrobacter succinogenes assimilated more ammonia than could be accounted for by cellular protein, RNA, or DNA and released large amounts of nonammonia nitrogen. The difference between net and true growth was most dramatic at low dilution rates, but mathematical derivations indicated that the lysis rate was a growth rate-independent funct
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10. Human neutrophil azurocidin synergizes with leukocyte elastase and cathepsin G in the killing of Capnocytophaga sputigena.
Azurocidin was purified in the presence of phenylmethylsulfonyl fluoride. Electrophoresis revealed at least seven species which exhibited N-terminal sequences consistent with azurocidin. Azurocidin exhibited no bactericidal activity against Capnocytophaga sputigena or other oral bacteria but synergized the bactericidal activity of enzymatically active elasta
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11. Role of protein subunits in Proteus rettgeri penicillin G acylase.
Penicillin G acylase from Proteus rettgeri is an 80,000- to 90,000-dalton enzyme composed of two nonidentical subunits. Both subunits were required for enzymatic activity. The 65,000-dalton beta subunit contained a phenylmethylsulfonyl fluoride-sensitive residue required for enzymatic activity, and the 24,500-dalton alpha subunit contained the domain that im
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12. Hemoglobin-digesting Acid Proteinases in Soybean Leaves: CHARACTERISTICS AND CHANGES DURING LEAF MATURATION AND SENESCENCE 1
Three proteinases which digest hemoglobin rapidly at acid pH (3.5 to 4.5) were identified in crude extracts of soybean (Merr.) leaves and separated by chromatography on DEAE-cellulose. All three enzymes were endopeptidases as judged by the ratio of α-amino-nitrogen plus peptide nitrogen over α-amino-nitrogen in the trichloroacetic acid-soluble portion of h